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The map label for this gene is ogt [H]

Identifier: 161870389

GI number: 161870389

Start: 1456387

End: 1457139

Strand: Reverse

Name: ogt [H]

Synonym: NMCC_1436

Alternate gene names: 161870389

Gene position: 1457139-1456387 (Counterclockwise)

Preceding gene: 161870390

Following gene: 161870388

Centisome position: 67.67

GC content: 55.38

Gene sequence:

>753_bases
ATGATTACCCTGCCTTCCTTAAACAATCTGCCGTCAAAATGGGATGAAATCCGCTATTGGCTCGAAACCCGGGTTTGTGA
GTGCGGCGTTATGCCGCATCCCAATTTGACGGAATGCGAAGCCGGGCAGTTCGATCGGGATTTTTGGGACAATATCGGCT
GCGCGCCTGAAGAATATGTCCGTATCCGCCGAGCCATCCGTCTGTTGGAAGCGCGTTATCCCGACAGTTTGAATGAGCTG
GTGTGTGCCGCCATTGCCACGCCTTTGGGCGAGATGCTGGCGGTGTTCGGAAATAAGGGATTGTGTCTGCTGGAGTTTGT
CGGACAGAAGCATTTGGAACAGGAAATTGCCGCCGTCCAAAAAGCCTTGCGCGGACGGTTTGTGTTTCGGGAGGATGAGC
GGACGCAACTTTTGCGGCAGGAATTGGACTTATACTTCAAGGGTCGTCTGAAAACCTTTGCCACGCCTTTGGAGCAGATT
GGCACCGAGTTTCAAAAGCAGGCATGGGACGCGCTCTTGGCGATTCCTTACGGCGAAACGCGCAGCTACAAAGAGCAGGC
GCAGCGTTTGGGCAACCCCAACGCCGTCCGCGCCGTTGCCGCCGCCAACGGGCAGAACAAGGTGTCTATCTTGATTCCCT
GCCACCGCGTCATCGGCAGCGACGGCAAACTGACCGGCTACGCGGGCGGGTTGAACCGCAAACAGTTTTTACTGGCTTTG
GAACGCGGCGAAGTTCAGACGGCATTGTTTTAA

Upstream 100 bases:

>100_bases
TCAAGATTTACGTCGTAGCCCGTTCCTGCATCAGCGTGCGGGAATGGCGAAAATTGGGAATTTTGCCGTCAAATCCATGA
TAGAGATACGGAGCAAACGA

Downstream 100 bases:

>100_bases
GCGGGGGAGGGCAGAACGGCTCATCGGGCTTAAATATGGTGTGATGACGGCTGTGGAAATTCATACAACGGCAATCCTTT
TAAGGCATCGGTCTTAATGT

Product: methylated-DNA-protein- cysteinemethyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MITLPSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQFDRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNEL
VCAAIATPLGEMLAVFGNKGLCLLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPNAVRAVAAANGQNKVSILIPCHRVIGSDGKLTGYAGGLNRKQFLLAL
ERGEVQTALF

Sequences:

>Translated_250_residues
MITLPSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQFDRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNEL
VCAAIATPLGEMLAVFGNKGLCLLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPNAVRAVAAANGQNKVSILIPCHRVIGSDGKLTGYAGGLNRKQFLLAL
ERGEVQTALF
>Mature_250_residues
MITLPSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQFDRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNEL
VCAAIATPLGEMLAVFGNKGLCLLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPNAVRAVAAANGQNKVSILIPCHRVIGSDGKLTGYAGGLNRKQFLLAL
ERGEVQTALF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=80, Percent_Identity=47.5, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=49.5238095238095, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788542, Length=157, Percent_Identity=38.2165605095541, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=89, Percent_Identity=47.1910112359551, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=89, Percent_Identity=47.1910112359551, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=90, Percent_Identity=42.2222222222222, Blast_Score=83, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17137554, Length=188, Percent_Identity=31.3829787234043, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 28271; Mature: 28271

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLPSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQFDRDFWDNIGCAPEEYV
CEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHH
RIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLCLLEFVGQKHLEQEIAAVQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQIGTEFQKQAWDALLAIPYGET
HHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
RSYKEQAQRLGNPNAVRAVAAANGQNKVSILIPCHRVIGSDGKLTGYAGGLNRKQFLLAL
CHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEHHHHHCCCCCEEEECCCCCHHHHHEEH
ERGEVQTALF
HCCCCHHHCC
>Mature Secondary Structure
MITLPSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQFDRDFWDNIGCAPEEYV
CEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHH
RIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLCLLEFVGQKHLEQEIAAVQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQIGTEFQKQAWDALLAIPYGET
HHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
RSYKEQAQRLGNPNAVRAVAAANGQNKVSILIPCHRVIGSDGKLTGYAGGLNRKQFLLAL
CHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEHHHHHCCCCCEEEECCCCCHHHHHEEH
ERGEVQTALF
HCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA