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The map label for this gene is ispF [H]

Identifier: 161870373

GI number: 161870373

Start: 1441332

End: 1441814

Strand: Reverse

Name: ispF [H]

Synonym: NMCC_1417

Alternate gene names: 161870373

Gene position: 1441814-1441332 (Counterclockwise)

Preceding gene: 161871033

Following gene: 161870372

Centisome position: 66.95

GC content: 56.31

Gene sequence:

>483_bases
ATGACGAACATCCGTATCGGACAGGGCTACGATGTCCACCAACTGACCGAAGGCAGAAAGCTGATACTTGGCGGAGTTGA
AATCCCATTTGAAAAAGGGCTGCTCGGACACTCCGATGCCGATGCGCTGCTGCACGCTGTTACCGACGCGCTGCTCGGTG
CGGCAGGTTTGGGCGATATCGGCAGCCATTTCCCCGACACCGCCGCAGAGTTCAAAGATGCGGACAGCCGCGTCCTTTTG
CGTGCGGCGTATCAAAGCGTTCAGGCGCAGGGTTGGCAGGCGGTCAATGTCGATACGACCGTGATTGCACAGAAACCCAA
ACTCGCGCCGCATATTCCGCAAATGCGTGCCAACATCGCCGCCGATTTGGGCATAGACATTTCGTGCGTCAACATCAAAG
GCAAAACCAACGAAAAACTCGGTTATCTCGGGCGCATGGAAGGCATAGAGGCGCAGGCGGCGGTATTGCTGGTAAGGATT
TGA

Upstream 100 bases:

>100_bases
GCGCGCAATTTGAAACTGACGCAGCCGCAGGACGCATACATCGTCAGGCTGCTGCTCAATGCCGTCTGAACTTCAATTCA
AAACATCAGGAACACAAACG

Downstream 100 bases:

>100_bases
ACGCGGCGGGCAGGACGTGCAAAAAAATGTTAAGATGGCGGCTGTTTGTTGATACGGTTTCCCAAAGGACAAAAATATGA
CGACACAAGACGAACTCAAG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALLHAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLL
RAAYQSVQAQGWQAVNVDTTVIAQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI

Sequences:

>Translated_160_residues
MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALLHAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLL
RAAYQSVQAQGWQAVNVDTTVIAQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
>Mature_159_residues
TNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALLHAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLR
AAYQSVQAQGWQAVNVDTTVIAQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=58.974358974359, Blast_Score=179, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17020; Mature: 16889

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALLHAVTDALLGAAGLGDI
CCCCCCCCCCCHHHHCCCCEEEEECCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCH
GSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVIAQKPKLAPHIPQMRANIA
HCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCCCCCHHHHHHH
ADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
HCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCEEEEEEEC
>Mature Secondary Structure 
TNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALLHAVTDALLGAAGLGDI
CCCCCCCCCCHHHHCCCCEEEEECCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCH
GSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVIAQKPKLAPHIPQMRANIA
HCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCCCCCHHHHHHH
ADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
HCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA