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The map label for this gene is pcnB [H]

Identifier: 161869779

GI number: 161869779

Start: 814242

End: 815603

Strand: Direct

Name: pcnB [H]

Synonym: NMCC_0802

Alternate gene names: 161869779

Gene position: 814242-815603 (Clockwise)

Preceding gene: 161869778

Following gene: 161869780

Centisome position: 37.81

GC content: 55.65

Gene sequence:

>1362_bases
ATGCTGAAAAAATGGTTGAATAAGATGCTGCCTTCCGGTCGGAGCAGTAAAAAAGCGGAAAGTAAAACGATCATTCCTGC
CGAAAGACACAACATCCGTGCCGAAATGTTGAGCTTTGCCGCCGAAAACGTCATACGCCGCCTGAAAGGGGCGGGGTTTC
AGGCTTATGTGGTCGGCGGCGCGGTCAGGGACCTGCTGCTCGGCATCGAACCCAAGGATTTCGATGTCGCAACCGATGCC
ACGCCCGAACAGGTGCACAAACTCTTCCGCCGCAGCCGCATCATCGGCAGGCGTTTTCAGATTGTCCATGTGATGAACGG
TGCGGAGATTATCGAAGTAACGACGTTTCGCGGCGGTGCGAAGGTTCATCAGAACGCGCACGGCAGAATCATGAAGGACA
ACACCTACGGCAGCATCGAAGAAGATGCGATGCGGCGCGATTTTACCTGCAATGCCTTGTATTACGATCCTGAAAAAGAA
GAGATTTTGGATTTCCACAACGGGATTGCCGATGTTGCCGCCCGCAGGCTGGTTATGATTGGTGATGCCGCCGAACGCTA
TCAGGAAGACCCTGTCAGGATTTTGCGCGCCATCCGCCTGTCGAGCAAATTGGGCTTTGATCTGTCGGAAGAAACCGCCG
CACCGATTGCCGAATCGATATGCCGTCTGAAGCACGAACCGGTAGCGCGGCTGTTCGACGAAATTATGAAACTGCTGTTT
TCAGGGCACGCGCGCGAGTGTCTGAAACGTTTGAACGGATTTGACATACCGGACGACATCCATCCGCTGCTCAATGCCTT
GCGCGTTTCAGACGGCATCGCCGGAAAAATGACGGTGCTTGCCCTGAAAAATACCGATGAGCGGCTGCGTGCCGACAAAT
CGGTTTCGGTCGGTTTCGTACTGGCGACCCTGATGTGGCCCGAGTTGGAACGCCATTGGAAAAGCAATCTGCAACAGGGT
TTGAAACCCGTGCCCGCCCTGTCCGATGCAATCAATACGATGCGCGAAACCGTCGAACGCGGTTGGGGCGTACCGCAACG
CTTTTCCGCCACGATGCGCGAAATTTGGATGTTCCAGCCGCAGTTTGAAAACCGCAAAGGCGCAAGGCCGCACAAACTGT
TTGCACAGGCGCGTTTCCGTGCCGCCTATGATTTCCTGCTCTTGCGCGCCGAAACCGGCAATGCGGACCGCGCCCTTGCC
GAGTGGTGGACGGCGTTTCAGACGGCATCGACGGAACAGCGGTCGGAGATGACCAAAAACGAAGCCGCCGCCCGACACGA
AAAAAACGAAGGACAGGCGAAAAAACGCCGCCGTCGCAGGCGCAAACCCAAGCCGAAGGTTGTGGGAACGGATTGGGAAT
AA

Upstream 100 bases:

>100_bases
GCCTTTTGTTTATGGTTACGGCATCTTGCGGAATCGTACCGCCTGATGCCGTCTGAACACGGTTGCCCGTGGAGATGCCG
CTCTTCGGGTTAGAATATTT

Downstream 100 bases:

>100_bases
GGGTCAACAGACAAGGAGCAATGAAGTTTTAACACATGGGGTGAAGCATAAAGTGCCGTTCTATGTATTATCCTGATTTG
TAAGGGGCCTCATCTCCGTA

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENVIRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDA
TPEQVHKLFRRSRIIGRRFQIVHVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSSKLGFDLSEETAAPIAESICRLKHEPVARLFDEIMKLLF
SGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGKMTVLALKNTDERLRADKSVSVGFVLATLMWPELERHWKSNLQQG
LKPVPALSDAINTMRETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAETGNADRALA
EWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGTDWE

Sequences:

>Translated_453_residues
MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENVIRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDA
TPEQVHKLFRRSRIIGRRFQIVHVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSSKLGFDLSEETAAPIAESICRLKHEPVARLFDEIMKLLF
SGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGKMTVLALKNTDERLRADKSVSVGFVLATLMWPELERHWKSNLQQG
LKPVPALSDAINTMRETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAETGNADRALA
EWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGTDWE
>Mature_453_residues
MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENVIRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDA
TPEQVHKLFRRSRIIGRRFQIVHVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSSKLGFDLSEETAAPIAESICRLKHEPVARLFDEIMKLLF
SGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGKMTVLALKNTDERLRADKSVSVGFVLATLMWPELERHWKSNLQQG
LKPVPALSDAINTMRETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAETGNADRALA
EWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGTDWE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=208, Percent_Identity=30.7692307692308, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI87081691, Length=408, Percent_Identity=41.6666666666667, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1789436, Length=167, Percent_Identity=35.3293413173653, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=207, Percent_Identity=32.8502415458937, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI281360112, Length=241, Percent_Identity=30.7053941908714, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357337, Length=241, Percent_Identity=30.7053941908714, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 51709; Mature: 51709

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS00036 BZIP_BASIC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENVIRRLKGAGFQAYVVGG
CCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHH
AVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIVHVMNGAEIIEVTTFRGGA
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEEECCCC
KVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKEEILDFHNGIADVAARRLVMI
HHCCCCCCCEEECCCCCCCHHHHHHCCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHEE
GDAAERYQEDPVRILRAIRLSSKLGFDLSEETAAPIAESICRLKHEPVARLFDEIMKLLF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGKMTVLALKNTDERLRADKSVSVGFV
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCCCCHHHHH
LATLMWPELERHWKSNLQQGLKPVPALSDAINTMRETVERGWGVPQRFSATMREIWMFQP
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
QFENRKGARPHKLFAQARFRAAYDFLLLRAETGNADRALAEWWTAFQTASTEQRSEMTKN
CCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
EAAARHEKNEGQAKKRRRRRRKPKPKVVGTDWE
HHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENVIRRLKGAGFQAYVVGG
CCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHH
AVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIVHVMNGAEIIEVTTFRGGA
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEEECCCC
KVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKEEILDFHNGIADVAARRLVMI
HHCCCCCCCEEECCCCCCCHHHHHHCCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHEE
GDAAERYQEDPVRILRAIRLSSKLGFDLSEETAAPIAESICRLKHEPVARLFDEIMKLLF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGKMTVLALKNTDERLRADKSVSVGFV
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCCCCHHHHH
LATLMWPELERHWKSNLQQGLKPVPALSDAINTMRETVERGWGVPQRFSATMREIWMFQP
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
QFENRKGARPHKLFAQARFRAAYDFLLLRAETGNADRALAEWWTAFQTASTEQRSEMTKN
CCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
EAAARHEKNEGQAKKRRRRRRKPKPKVVGTDWE
HHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]