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The map label for this gene is purA [H]

Identifier: 161869759

GI number: 161869759

Start: 785380

End: 786678

Strand: Direct

Name: purA [H]

Synonym: NMCC_0779

Alternate gene names: 161869759

Gene position: 785380-786678 (Clockwise)

Preceding gene: 161869758

Following gene: 161869760

Centisome position: 36.47

GC content: 56.51

Gene sequence:

>1299_bases
ATGGCTAAAAATGTTGTAGTAATCGGCGCACAGTGGGGCGACGAGGGTAAAGGTAAAATCGTTGACTGGCTGGCGGAAGA
AGCCGGCGGCGTGGTGCGCTTCCAAGGCGGCCACAATGCGGGCCATACCTTGGTTGTCGGCGGCAAAAAAACCATTTTGC
GCCTGATTCCGAGCGGCATCCTGCATGAAAGTTTGGACTGCTTCATCGGTTCGGGCGTTGTCGTCTCCCCCGAAGCCCTG
TTGGGCGAAATCGACGAGTTGAACGCGGCAGGCGTGAAAAACGTCGAAGGCCGTCTGAAAATCGCGCCGACCTGCCCGCT
GATCCTGCCTTATCACATCGCGCTCGACCAAGCCCGCGAAGCATCGCGCGGCAAAGGCAAAATCGGCACGACCGGCCGCG
GCATCGGCCCTGCCTACGAAGACAAAGTGGCACGCCGCGCCATTCGCGCCGCCGATTTGCTGCATCCTGAAAAACTGCGT
GAAAAACTGGATGCCGTCCTTGCCTATTACAATGTCCAACTGCAACATCTGCACAATGCCGAGCCGGTTAAAGCGGAAGA
CGTGATGGCGGTTATCGAAAAAGTCGCGCCGCGCATTGCGCCGATGATTACCGACGTGTCCCGCGTGTTGAACGAGAAAA
ACAAAAACGGCGAAAAACTGCTGTTTGAAGGCGCGCAAGGTGCGTTGTTGGACATCGACTACGGCACTTATCCCTTCGTT
ACCTCGTCCAACTGTCTGGCGGGAGCCGCTTCCGCAGGCGCGGGCGTAGGTCCTCAAATGCTGGATTATGTTTTGGGCAT
CGTCAAAGCCTATACCACGCGCGTCGGTTCAGGCCCGTTCCCGACCGAATTGTTCGACGAAGTAGGCGCAGGTTTGGCGG
AACGCGGACACGAATTCGGCTCGGTAACCGGCCGCGCACGCCGCTGCGGCTGGTTTGATGCCGCCGCCCTGAAACGCTCC
ATCCAAATCAACGGCATTTCCGGTATGTGCATTACCAAACTCGATGTAATGGACGGCGTTGAAACCATCAATATCTGCGT
CGGCTACGAATTGCCCGACGGCGGCAAAACCGATATCCTGCCTTGCGGTTCGGATGCGGTGGAAACCTGCAAACCGATTT
ACGAAACAATGGAGGGTTGGAAAGAATCGACTTTCGGCATCAAAAATTATGAGGAACTGCCCGATAATGCCAAAGCATAT
TTGAAACGGATTGAAGAAGTCTGCGGCGCGCCGGTCGCCATCGTCTCCACCGGCCCCGACCGCGAAGAAACGATTGTGCT
GCATCATCCGTTCGCATAA

Upstream 100 bases:

>100_bases
CCCGTTCATGGCGGATGAAAGGCAAATCGTGGCGGGGCGCAAAGCCGCACCGGTTTGGGGATTTCCGCAATAATTTTTAA
TATCGATAGGTTATATGGCT

Downstream 100 bases:

>100_bases
GGTTTTGCAGTAAAATTGCCGTCTGAAGCCCTAATCCGCCGTTGGTCATAAATAGTAGGGTATCAACATTTCGGGCTACA
ATGGTACGTCAGCCAATGCC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTLVVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEAL
LGEIDELNAAGVKNVEGRLKIAPTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGEKLLFEGAQGALLDIDYGTYPFV
TSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVGSGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRS
IQINGISGMCITKLDVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMEGWKESTFGIKNYEELPDNAKAY
LKRIEEVCGAPVAIVSTGPDREETIVLHHPFA

Sequences:

>Translated_432_residues
MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTLVVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEAL
LGEIDELNAAGVKNVEGRLKIAPTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGEKLLFEGAQGALLDIDYGTYPFV
TSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVGSGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRS
IQINGISGMCITKLDVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMEGWKESTFGIKNYEELPDNAKAY
LKRIEEVCGAPVAIVSTGPDREETIVLHHPFA
>Mature_431_residues
AKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTLVVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALL
GEIDELNAAGVKNVEGRLKIAPTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLRE
KLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGEKLLFEGAQGALLDIDYGTYPFVT
SSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVGSGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSI
QINGISGMCITKLDVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMEGWKESTFGIKNYEELPDNAKAYL
KRIEEVCGAPVAIVSTGPDREETIVLHHPFA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=431, Percent_Identity=41.9953596287703, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI34577063, Length=432, Percent_Identity=40.7407407407407, Blast_Score=302, Evalue=3e-82,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=39.9497487437186, Blast_Score=283, Evalue=2e-76,
Organism=Escherichia coli, GI1790620, Length=431, Percent_Identity=56.3805104408353, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI25146553, Length=427, Percent_Identity=41.4519906323185, Blast_Score=320, Evalue=9e-88,
Organism=Caenorhabditis elegans, GI25146555, Length=427, Percent_Identity=41.4519906323185, Blast_Score=320, Evalue=1e-87,
Organism=Saccharomyces cerevisiae, GI6324109, Length=427, Percent_Identity=40.0468384074941, Blast_Score=313, Evalue=3e-86,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=41.5094339622642, Blast_Score=335, Evalue=4e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46153; Mature: 46022

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTLVVGGKKTILRLIPSGI
CCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCEEEECCHHHHHHHHHHHH
LHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIAPTCPLILPYHIALDQARE
HHHHHHHHHCCCEEECCHHHHCCHHHCCCCCCCCCCCCEEECCCCCEEEHHHHHHHHHHH
ASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLREKLDAVLAYYNVQLQHLHNA
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCC
EPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGEKLLFEGAQGALLDIDYGTYPFV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEECCCCCCCE
TSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVGSGPFPTELFDEVGAGLAERGHEFG
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHCCCHHH
SVTGRARRCGWFDAAALKRSIQINGISGMCITKLDVMDGVETINICVGYELPDGGKTDIL
CCCCCHHHCCCHHHHHHHHEEEECCCCCCCEEHHHHHCCCEEEEEEEEEECCCCCCCCEE
PCGSDAVETCKPIYETMEGWKESTFGIKNYEELPDNAKAYLKRIEEVCGAPVAIVSTGPD
ECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHCCCEEEEECCCC
REETIVLHHPFA
CCCEEEEECCCC
>Mature Secondary Structure 
AKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTLVVGGKKTILRLIPSGI
CCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCEEEECCHHHHHHHHHHHH
LHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIAPTCPLILPYHIALDQARE
HHHHHHHHHCCCEEECCHHHHCCHHHCCCCCCCCCCCCEEECCCCCEEEHHHHHHHHHHH
ASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLREKLDAVLAYYNVQLQHLHNA
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCC
EPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGEKLLFEGAQGALLDIDYGTYPFV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEECCCCCCCE
TSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVGSGPFPTELFDEVGAGLAERGHEFG
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHCCCHHH
SVTGRARRCGWFDAAALKRSIQINGISGMCITKLDVMDGVETINICVGYELPDGGKTDIL
CCCCCHHHCCCHHHHHHHHEEEECCCCCCCEEHHHHHCCCEEEEEEEEEECCCCCCCCEE
PCGSDAVETCKPIYETMEGWKESTFGIKNYEELPDNAKAYLKRIEEVCGAPVAIVSTGPD
ECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHCCCEEEEECCCC
REETIVLHHPFA
CCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA