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The map label for this gene is pheS [H]

Identifier: 161869666

GI number: 161869666

Start: 696292

End: 697350

Strand: Direct

Name: pheS [H]

Synonym: NMCC_0684

Alternate gene names: 161869666

Gene position: 696292-697350 (Clockwise)

Preceding gene: 161869665

Following gene: 161869667

Centisome position: 32.33

GC content: 50.05

Gene sequence:

>1059_bases
ATGTCAGGCTATACAGGCGGCCTCGTTGTTTCAGGTGGTATATCATTAATTGACAGACTTGATATTATGGAAAATGTAAA
CCGCATCGTTGCAGAAGGCATTGCCGCAGTAGAAGCTGCGCAAGACTTCAACGCTCTAGAACAAATCAAAGCCCGTTATC
TTGGCAAAACCGGCGAGTTGACCGGACTTCTGAAAACTTTGGGGCAAATGTCGCCTGAAGAGCGCAAAACCATAGGTGCG
CATATCAATGAATGCAAAAACCGGTTTCAGACGGCTTTTAATGCCAAACGCGATGCCCTCAACGAAGTCAAGCTGCAAGC
CCGACTTGCCGCCGAAGCCCTCGATATTACCCTGCCCGGACGCGCTCAGGAAGGCGGCAGCCTGCATCCCGTAACCCTGA
CCTTGCAACGTGTGGTCGAACTCTTTCACGGAATGGGTTTCGAAGTGGCGGACGGGCCTGAAATCGAAGACGATTTTCAC
AATTTCCAAGCCCTGAACATCCCTGCAAACCATCCTGCCCGTGCGATGCAGGATACGTTTTACGTTGAAAACGGCGATGT
TTTGCGTACGCACACTTCCCCGATTCAAATCCGCTATATGCTCGATAAAAAAGAGCCGCCCATCCGCATTATCGCCCCCG
GCCGCGTTTACCGTGTGGACAGCGATGCCACGCACTCGCCTATGTTCCATCAGGCGGAAGGTTTGTGGGTAGAAGAGGGC
GTAACTTTTGCCGATTTGAAAGCAGTGTTCACGGATTTTATCCGTCGCTTCTTTGAACGCGATGATTTGCAAGTACGTTT
CCGCCCGTCTTTCTTCCCGTTCACCGAACCGTCTGCCGAAATCGACATTATGGGCGAAAACGGCAAATGGCTGGAAGTCG
GCGGCTGCGGCATGGTGCATCCCAACGTGTTGAAAAATGTGAATATCGATTCTGAAAAATACACCGGTTTCGCTTTCGGT
ATCGGTCTCGACCGTTTCGCGATGCTGCGTTACAACGTGAACGATTTGCGCTTGTTTTTTGATAACGATTTGAATTTTTT
GAAGCAGTTTGTAAAATGA

Upstream 100 bases:

>100_bases
GGCGAGGCAACGCCGTACTGGTTTAAATTTAATCCACTATACAGAAAATTTATCCAATGGATTGACCGTGAAGAAAATAA
GGTCGTCTGAAGAGTCTGAT

Downstream 100 bases:

>100_bases
TTGTGTAGGCTGCCTAAAAATGAAAAAAGCAGCCTGTGCCTACCTCAGAAAGCTAAATCATGAAACGCTATGTCGCTCCA
TTATCCGCCTGCTATGCGTT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSGYTGGLVVSGGISLIDRLDIMENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKTLGQMSPEERKTIGA
HINECKNRFQTAFNAKRDALNEVKLQARLAAEALDITLPGRAQEGGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFH
NFQALNIPANHPARAMQDTFYVENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGMVHPNVLKNVNIDSEKYTGFAFG
IGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK

Sequences:

>Translated_352_residues
MSGYTGGLVVSGGISLIDRLDIMENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKTLGQMSPEERKTIGA
HINECKNRFQTAFNAKRDALNEVKLQARLAAEALDITLPGRAQEGGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFH
NFQALNIPANHPARAMQDTFYVENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGMVHPNVLKNVNIDSEKYTGFAFG
IGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK
>Mature_351_residues
SGYTGGLVVSGGISLIDRLDIMENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKTLGQMSPEERKTIGAH
INECKNRFQTAFNAKRDALNEVKLQARLAAEALDITLPGRAQEGGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHN
FQALNIPANHPARAMQDTFYVENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEGV
TFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGMVHPNVLKNVNIDSEKYTGFAFGI
GLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=284, Percent_Identity=31.3380281690141, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI4758340, Length=269, Percent_Identity=30.1115241635688, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1788007, Length=328, Percent_Identity=59.1463414634146, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32563657, Length=275, Percent_Identity=29.4545454545455, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17508957, Length=275, Percent_Identity=29.4545454545455, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32566635, Length=276, Percent_Identity=30.7971014492754, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321087, Length=259, Percent_Identity=32.8185328185328, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=91, Percent_Identity=41.7582417582418, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137424, Length=283, Percent_Identity=30.7420494699647, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18858079, Length=259, Percent_Identity=29.7297297297297, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39544; Mature: 39412

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGYTGGLVVSGGISLIDRLDIMENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGEL
CCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
TGLLKTLGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEVKLQARLAAEALDITLPG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
RAQEGGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTF
CCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCHHHCCEEEEECCCCCCHHHHHHEE
YVENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
EEECCCEEEECCCCEEEEEEECCCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCHHCC
VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGMVH
CCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEECCCCCEEEECCCCCCC
PNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK
CHHHHCCCCCCCCCCEEEEECCHHHEEEEEECCCEEEEEECCCHHHHHHHHC
>Mature Secondary Structure 
SGYTGGLVVSGGISLIDRLDIMENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGEL
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
TGLLKTLGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEVKLQARLAAEALDITLPG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
RAQEGGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTF
CCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCHHHCCEEEEECCCCCCHHHHHHEE
YVENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
EEECCCEEEECCCCEEEEEEECCCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCHHCC
VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGMVH
CCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEECCCCCEEEECCCCCCC
PNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK
CHHHHCCCCCCCCCCEEEEECCHHHEEEEEECCCEEEEEECCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10722605; 10761919 [H]