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The map label for this gene is ksgA [H]

Identifier: 161869639

GI number: 161869639

Start: 663870

End: 664649

Strand: Reverse

Name: ksgA [H]

Synonym: NMCC_0656

Alternate gene names: 161869639

Gene position: 664649-663870 (Counterclockwise)

Preceding gene: 161869642

Following gene: 161869638

Centisome position: 30.86

GC content: 53.85

Gene sequence:

>780_bases
ATGAAAGAACACAAAGCCCGCAAGCGTTTCGGGCAGAATTTTTTGCAGGACACGCGGATTATCAGCGATATTGTCAACGC
CGTGCGCCCGCAGGCGGATGATGTCGTGATTGAAATCGGCCCGGGTTTGGCGGCGATTACCGAACCTTTGGCGAAAAAGC
TGAACCGCCTGCACGTTGTCGAAATCGACCGCGACATCGTATGCCGTCTGAAAACGCTGCCGTTTGCGGATAAACTGGTG
ATTCACGAAGGCGATGTATTGCAGTTTGATTTCAACGGCATCGCAGGCAAAAAGAAAATCGTCGGCAACCTGCCCTACAA
CATTTCCACGCCGCTTTTGTTCAAGCTGGCGGAGGTGGCGGACGATGTCGTCGATATGCACTTTATGCTGCAAAAAGAAG
TGGTCGATCGTATGGTTGCCGCGCCGAAAAGCAACGACTACGGCCGCTTGGGCGTGATGTTGCAATATTTCTTCGATATG
GAGCTGCTGATTGACGTGCCGCCCGAATCGTTCGACCCCGCGCCGAAAGTGGATTCCGCCGTCGTCCGTATGATTCCGGT
GAAACACCGCATCGGCAAGGCGGACGATTTCGAGCATTTCGCCAAACTGGTGAAACTCGCCTTCCACCAGCGGCGCAAAA
CCATACGCAACAACCTGAAAGAGCTTGCAGGCGACGATGATTTGCAGGCAGTCGACATCAATCCGCAAGACCGTGCCGAA
CATATCGCGCCGGAGAAATATGTGGCGTTGAGCAATTATCTAGCGGGCAAGGCCGTCTGA

Upstream 100 bases:

>100_bases
CCCTATGTGCCATCCTGATTTGGAAGGGGTTACGCCCCTCCCAAATAGAGTCTGATTCTACCGCCCTAAAGGGCGGGGTT
TCAACCGAAAAGGAAACACG

Downstream 100 bases:

>100_bases
AAAGGGAGTAAGCCGATGACGAAATACCTGCTTTTACCCCTGATACTGCCGCTTGCAGCCTGCCTGACCAATCCGAACGA
CCGTTTTTTTGACGGCAGAC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLAAITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLV
IHEGDVLQFDFNGIAGKKKIVGNLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
ELLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNNLKELAGDDDLQAVDINPQDRAE
HIAPEKYVALSNYLAGKAV

Sequences:

>Translated_259_residues
MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLAAITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLV
IHEGDVLQFDFNGIAGKKKIVGNLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
ELLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNNLKELAGDDDLQAVDINPQDRAE
HIAPEKYVALSNYLAGKAV
>Mature_259_residues
MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLAAITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLV
IHEGDVLQFDFNGIAGKKKIVGNLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
ELLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNNLKELAGDDDLQAVDINPQDRAE
HIAPEKYVALSNYLAGKAV

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=217, Percent_Identity=32.7188940092166, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI156415992, Length=280, Percent_Identity=30, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1786236, Length=260, Percent_Identity=49.2307692307692, Blast_Score=258, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI25146882, Length=209, Percent_Identity=33.0143540669856, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25141369, Length=287, Percent_Identity=27.8745644599303, Blast_Score=101, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=229, Percent_Identity=27.5109170305677, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21358017, Length=222, Percent_Identity=30.6306306306306, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21357273, Length=258, Percent_Identity=31.0077519379845, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 29214; Mature: 29214

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLAAITEPLAKKLNRLHVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCEEEE
EIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVGNLPYNISTPLLFKLAEVA
EECHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHH
DDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDMELLIDVPPESFDPAPKVDSA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHH
VVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNNLKELAGDDDLQAVDINPQDRAE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHH
HIAPEKYVALSNYLAGKAV
HCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLAAITEPLAKKLNRLHVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCEEEE
EIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVGNLPYNISTPLLFKLAEVA
EECHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHH
DDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDMELLIDVPPESFDPAPKVDSA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHH
VVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNNLKELAGDDDLQAVDINPQDRAE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHH
HIAPEKYVALSNYLAGKAV
HCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA