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The map label for this gene is folC [H]

Identifier: 161869635

GI number: 161869635

Start: 660786

End: 662006

Strand: Reverse

Name: folC [H]

Synonym: NMCC_0652

Alternate gene names: 161869635

Gene position: 662006-660786 (Counterclockwise)

Preceding gene: 161869636

Following gene: 161869634

Centisome position: 30.74

GC content: 56.18

Gene sequence:

>1221_bases
TTGATCGACATGGGTTTGGAGCGCACGAGCGAAGTGAAAAAACGCATGAAGCTCGAGCCGCAATGCCCCGTCGTCGTTGT
CGCGGGAACCAACGGCAAAGGTTCGGTCTGTGCCTACCTGACACAGATTTACAAACAGGCGGGCTACAAAACCGGTACGC
TGACCAGCCCGCATTTATTACGTTACAACGAACGCATCGCTGTCAACGCCGAACCCGTTTCGGACGATACGATTATTGCC
TCTTTCGAGCGCATCGAAGCGGCGCGCGGCGAAATATCACTGACTTATTTTGAATTCAATGCCTTGGCGGCCGTCGACAT
CTTCATGCGCGAACAGGTTGACGTGATGATATTGGAAGTCGGCTTGGGCGGACGCTTGGACGCGGTCAACGCATTTGACG
GCGATTGCGCGGTGGTTACCAGCGTGGATTTGGACCATCAGGCATTTTTGGGCGATACGGTCGAGCAGGTCGGCTTTGAA
AAAGCAGGCGTGTTCCGCGGCGGCAAACCCGCCATCTGCGGTCAAAACCCCGCGCCCGCATCGTTGCTTTCACACGCCGA
AGCCATAGGCGCGAAACTGCTGATGGTGCAGCGCGATTTCGAGTTTCACGCGATGGAAAACATCCAATGGAACTACCGCT
TCCGGCCGCAGCATTCAGACGGTCCCGCACGCAACCGCAATGCCCTGCCTTTCCCCGCATTGCGTGGCGCATACCAGCTT
TCCAACGCCGCCTGCGCGTTGACCGTATTGGAATGCTTGAACGACAAATTGCCTGTGGACATCGGCGCCATCAAACGCGG
ATTGCTGCTGGTTGAAAATCCCGGACGCTTCCAAGTCCTGCCCGGCCGTCCGCTGACCGTTTTGGATGTCGGACACAATC
CCCACGCTGCCCGCGCCCTGCGCCGCAGCCTGATTAATTTGGCGTTTGCGCAAAAACGCACCGCCGTATTCAGTATGCTG
TCCGACAAAGACATAGACGGCGTGTTGGAAATCGTTAAAGACCAGTTTGACGAGTGGTATATCGCACCGTTGGACGTACC
GCGCGGCATGACGCTCGAGAGCCTGCAAAACAAACTGCACGAACACAGCATCGAAAACATACAGACATTCGCCGCCGTCC
GCGAAGCATACCGGGCGGCAGCCTCAAAAGCGGGCGAAAACGACAGAATCGTCGTTTTCGGCTCATTTCATACCGTTGCC
GATGTCATGTCGGTACTTTAA

Upstream 100 bases:

>100_bases
GATACGCCAAGCTGATTTCAGACGGCATTTGAACACGGAACACTCAATGAAAACACTGCAAGACTGGCTCTCGCATTTGG
AAACCGCCCACAGCGGCGGT

Downstream 100 bases:

>100_bases
AAGGAAACTTCTATGTCCGAAAACAAACAAAACGAAGTCCTGAGCGGTTACGAACAACTCAAACGGCGCAACCGCCGCCG
CCTCGTAACGGCAAGTTGCC

Product: bifunctional folylpolyglutamate synthase/dihydrofolate synthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MIDMGLERTSEVKKRMKLEPQCPVVVVAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAVNAEPVSDDTIIA
SFERIEAARGEISLTYFEFNALAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSVDLDHQAFLGDTVEQVGFE
KAGVFRGGKPAICGQNPAPASLLSHAEAIGAKLLMVQRDFEFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQL
SNAACALTVLECLNDKLPVDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAVFSML
SDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVREAYRAAASKAGENDRIVVFGSFHTVA
DVMSVL

Sequences:

>Translated_406_residues
MIDMGLERTSEVKKRMKLEPQCPVVVVAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAVNAEPVSDDTIIA
SFERIEAARGEISLTYFEFNALAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSVDLDHQAFLGDTVEQVGFE
KAGVFRGGKPAICGQNPAPASLLSHAEAIGAKLLMVQRDFEFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQL
SNAACALTVLECLNDKLPVDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAVFSML
SDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVREAYRAAASKAGENDRIVVFGSFHTVA
DVMSVL
>Mature_406_residues
MIDMGLERTSEVKKRMKLEPQCPVVVVAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAVNAEPVSDDTIIA
SFERIEAARGEISLTYFEFNALAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSVDLDHQAFLGDTVEQVGFE
KAGVFRGGKPAICGQNPAPASLLSHAEAIGAKLLMVQRDFEFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQL
SNAACALTVLECLNDKLPVDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAVFSML
SDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVREAYRAAASKAGENDRIVVFGSFHTVA
DVMSVL

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=358, Percent_Identity=30.7262569832402, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI66932990, Length=364, Percent_Identity=30.4945054945055, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1788654, Length=409, Percent_Identity=43.2762836185819, Blast_Score=292, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17553150, Length=338, Percent_Identity=28.698224852071, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71984923, Length=338, Percent_Identity=28.698224852071, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17553148, Length=338, Percent_Identity=28.698224852071, Blast_Score=125, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323760, Length=421, Percent_Identity=27.790973871734, Blast_Score=135, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6324815, Length=332, Percent_Identity=26.5060240963855, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24641571, Length=155, Percent_Identity=34.1935483870968, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24581568, Length=164, Percent_Identity=33.5365853658537, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 44580; Mature: 44580

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDMGLERTSEVKKRMKLEPQCPVVVVAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLL
CCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHE
RYNERIAVNAEPVSDDTIIASFERIEAARGEISLTYFEFNALAAVDIFMREQVDVMILEV
EECCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCEEEEEE
GLGGRLDAVNAFDGDCAVVTSVDLDHQAFLGDTVEQVGFEKAGVFRGGKPAICGQNPAPA
CCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCH
SLLSHAEAIGAKLLMVQRDFEFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQL
HHHHHHHHHHHEEEEEECCCCEEECCCCEEEEEECCCCCCCCCCCCCCCCCHHHHCHHHH
SNAACALTVLECLNDKLPVDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARAL
CCHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCEEEECCCCEEEEECCCCCHHHHHH
RRSLINLAFAQKRTAVFSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLH
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHH
EHSIENIQTFAAVREAYRAAASKAGENDRIVVFGSFHTVADVMSVL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHC
>Mature Secondary Structure
MIDMGLERTSEVKKRMKLEPQCPVVVVAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLL
CCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHE
RYNERIAVNAEPVSDDTIIASFERIEAARGEISLTYFEFNALAAVDIFMREQVDVMILEV
EECCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCEEEEEE
GLGGRLDAVNAFDGDCAVVTSVDLDHQAFLGDTVEQVGFEKAGVFRGGKPAICGQNPAPA
CCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCH
SLLSHAEAIGAKLLMVQRDFEFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQL
HHHHHHHHHHHEEEEEECCCCEEECCCCEEEEEECCCCCCCCCCCCCCCCCHHHHCHHHH
SNAACALTVLECLNDKLPVDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARAL
CCHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCEEEECCCCEEEEECCCCCHHHHHH
RRSLINLAFAQKRTAVFSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLH
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHH
EHSIENIQTFAAVREAYRAAASKAGENDRIVVFGSFHTVADVMSVL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]