Click here to switch to the map view.

The map label for this gene is potD2 [H]

Identifier: 161869556

GI number: 161869556

Start: 595584

End: 596744

Strand: Direct

Name: potD2 [H]

Synonym: NMCC_0571

Alternate gene names: 161869556

Gene position: 595584-596744 (Clockwise)

Preceding gene: 161869555

Following gene: 161869557

Centisome position: 27.66

GC content: 54.95

Gene sequence:

>1161_bases
TTGAACGAAAGGTCGAAAACCATGAAAAAAACACTGGTGGCGGCGGCAATCCTGAGCCTCGCCTTGACTGCGTGCGGCGG
CGGAAACGATACCGCCGCCCAAGCCCCCTCCGCCAAGCCCGAAGCCGAACAATCGGGCAAACTCAATATCTACAACTGGT
CGGATTATGTCGATCCCGAAACCGTCGCCGCCTTTGAAAAAGAAACCGGCATCAAGACGCGTTCCGATTATTACGACAGC
AACGAAACACTGGAGGCAAAAGTCCTGACCGGCAAATCCGGCTACGACCTGACCGCGCCGTCCATCGCCAACGTCGGCCG
GCAAATCAAAGCGGGCGCGTATCAGAAAATCGACAAGGCGCAAATCCCCCATTACGGCAACATCGATAAAGATTTGCTGA
AAATGATGGAAGCCGTCGATCCGGGCAACGAATACGCCGTCCCCTATTTCTGGGGCATCAACACCTTGGCAATCAACACG
GGGCAGGTGCAAAAGGCATTGGGTACGGACAAGCTGCCCGAAAACGAATGGGATTTGGTGTTCAAACCCGAATACACCGC
CAAACTCAAATCCTGCGGCATCAGCTATTTCGACAGCGCAATCGAACAGATTCCCTTGGCGTTGCACTATTTGGGCAAAG
ACCCCAACAGTGAGAATCCCGAAGACATCAAAGCCGCCGTCGATATGATGAAAGCCGTCCGGAGCGACGTGAAACGCTTC
AGCTCTTCCGGCTATATCGACGATATGGCGGCAGGCAACCTGTGTGCCGCCATCGGTTACGGCGGCGATTTGAACATTGC
CAAAACCCGTGCCGAAGAAGCGGCAAACGGCGTGGAAATCAAAGTATTGACCCCGAAAACCGGCGTGGGCGTGTGGGTAG
ATTCCTTTATGATTCCGCGCGACGCACAAAACGTTGCCAACGCCCACCGCTATATCGACTACACGCTCCGGCCCGAGGTG
GCGGCGAAAAACGGCAGCTTCGTTACCTACGCGCCCGCCAGCCGTCCCGCGCGCGAGCTGATGGATGAAAAATACACCTC
CGACGCATCGATTTTCCCGAACAAAGAACTGATGGAAAAAAGTTTCATCGTATCGCCCAAATCCGCAGAATCCGCCAAAC
TGGGCGTGAAGCTGTGGCAAGGGCTCAAGGCCGGAAAATAA

Upstream 100 bases:

>100_bases
TAATGCAAACCTTGCTGCACCAAGGGCCAAGAAAGCCGACCGGCCGCCCCCACAGCTTCCGATGCAGGCGGCCCGGCCGT
CCCTGCAATGTTTTTTATTT

Downstream 100 bases:

>100_bases
CCGCAACCCCTGCCGTCTGAAACCTTTCGGGCGGCAGGAAACGGCGCGTCCGTTATCAAACAGGGGGGGCGTTTCCCCTC
CTGCCGGTTATGATTGGGTT

Product: spermidine/putrescine ABC transporter, periplasmic spermidine/putrescine-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MNERSKTMKKTLVAAAILSLALTACGGGNDTAAQAPSAKPEAEQSGKLNIYNWSDYVDPETVAAFEKETGIKTRSDYYDS
NETLEAKVLTGKSGYDLTAPSIANVGRQIKAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINT
GQVQKALGTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKAAVDMMKAVRSDVKRF
SSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVLTPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEV
AAKNGSFVTYAPASRPARELMDEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK

Sequences:

>Translated_386_residues
MNERSKTMKKTLVAAAILSLALTACGGGNDTAAQAPSAKPEAEQSGKLNIYNWSDYVDPETVAAFEKETGIKTRSDYYDS
NETLEAKVLTGKSGYDLTAPSIANVGRQIKAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINT
GQVQKALGTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKAAVDMMKAVRSDVKRF
SSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVLTPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEV
AAKNGSFVTYAPASRPARELMDEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK
>Mature_386_residues
MNERSKTMKKTLVAAAILSLALTACGGGNDTAAQAPSAKPEAEQSGKLNIYNWSDYVDPETVAAFEKETGIKTRSDYYDS
NETLEAKVLTGKSGYDLTAPSIANVGRQIKAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINT
GQVQKALGTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKAAVDMMKAVRSDVKRF
SSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVLTPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEV
AAKNGSFVTYAPASRPARELMDEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=356, Percent_Identity=40.1685393258427, Blast_Score=290, Evalue=8e-80,
Organism=Escherichia coli, GI1787367, Length=347, Percent_Identity=32.2766570605187, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1787711, Length=361, Percent_Identity=26.8698060941828, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41983; Mature: 41983

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNERSKTMKKTLVAAAILSLALTACGGGNDTAAQAPSAKPEAEQSGKLNIYNWSDYVDPE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHH
TVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQIKAGAYQKIDKA
HHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
QIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTGQVQKALGTDKLPENEWDLV
CCCCCCCCCHHHHHHHHHCCCCCCEECEEEECCEEEEEECCHHHHHHCCCCCCCCCCCEE
FKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKAAVDMMKAVRSDVKRF
ECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVLTPKTGVGVWVDSFMIPR
HCCCCCCHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHCEECCC
DAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELMDEKYTSDASIFPNKELMEK
CHHHHHHHHHHEEEEECCCEECCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHH
SFIVSPKSAESAKLGVKLWQGLKAGK
HHCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNERSKTMKKTLVAAAILSLALTACGGGNDTAAQAPSAKPEAEQSGKLNIYNWSDYVDPE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHH
TVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQIKAGAYQKIDKA
HHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
QIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTGQVQKALGTDKLPENEWDLV
CCCCCCCCCHHHHHHHHHCCCCCCEECEEEECCEEEEEECCHHHHHHCCCCCCCCCCCEE
FKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKAAVDMMKAVRSDVKRF
ECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVLTPKTGVGVWVDSFMIPR
HCCCCCCHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHCEECCC
DAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELMDEKYTSDASIFPNKELMEK
CHHHHHHHHHHEEEEECCCEECCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHH
SFIVSPKSAESAKLGVKLWQGLKAGK
HHCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]