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The map label for this gene is pilQ [H]

Identifier: 161869408

GI number: 161869408

Start: 429526

End: 431811

Strand: Reverse

Name: pilQ [H]

Synonym: NMCC_0416

Alternate gene names: 161869408

Gene position: 431811-429526 (Counterclockwise)

Preceding gene: 161869409

Following gene: 161869407

Centisome position: 20.05

GC content: 52.49

Gene sequence:

>2286_bases
ATGAATACCAAACTGACAAAAATCATTTCCGGTCTCTTTGTCGCAACCGCCGCCTTTCAGACAGCATCGGCAGGAAACAT
TACAGACATCAAAGTTTCCTCCCTGCCCAACAAACAGAAAATCGTCAAAGTCAGCTTTGACAAAGAGATTGTCAACCCGA
CCGGCTTCGTAACCTCCTCACCGGCCCGCATCGCCTTGGACTTTGAACAAACCGGCATTTCCATGGATCAACAGGTACTC
GAATATGCCGATCCCCTGTTGAGCAAAATCAGTGCCGCACAAAACAGCAGCCGTGCGCGTTTGGTTCTGAATCTGAACAA
ACCGGGCCAATACAATACCGAAGTACGCGGGAACAAAGTTTGGATATTCATTAACGAATCGGACGATACCGTGTCCGCCC
CCGCACGCCCCGCCGTAAAAGCCGCGCCTGCCGCACCGGCAAAACAACAGGCTGCCGCACCGTCTACCAAGTCCGCAGTA
TCCGTATCCAAACCCTTTACCCCGGCAAAACAACAGGCTGCCGCACCGTTTACCGAGTCCGTAGTATCCGTATCCGCACC
GTTCAGCCCGGCAAAACAACAGGCGGCGGCATCAGCAAAACAACAGACGGCAGCACCAGCAAAACAACAGGCGGCAACAC
CAGCAAAACAAACCAATATCGATTTCCGCAAAGACGGCAAAAATGCCGGCATTATCGAATTGGCTGCATTGGGCTTTGCC
GGGCAGCCCGACATCAGCCAACAGCACGACCACATCATCGTTACGCTGAAAAACCATACCCTGCCGACCACGCTCCAACG
CAGTTTGGATGTGGCAGACTTTAAAACACCGGTTCAAAAGGTTACGCTGAAACGCCTCAATAACGACACCCAGCTGATTA
TCACAACAGCCGGCAACTGGGAACTCGTCAACAAATCCGCCGCGCCCGGATACTTTACCTTCCAAGTCCTGCCGAAAAAA
CAAAACCTCGAGTCAGGCGGCGTGAACAATGCGCCCAAAACCTTCACAGGCCGGAAAATCTCCCTTGACTTCCAAGATGT
CGAAATCCGCACCATCCTGCAGATTTTGGCAAAAGAATCCGGGATGAACATTGTTGCCAGCGACTCCGTCAACGGCAAAA
TGACCCTCTCCCTCAAAGACGTACCTTGGGATCAGGCTTTGGATTTGGTTATGCAGGCACGCAACCTCGATATGCGCCAG
CAAGGGAACATCGTCAACATCGCGCCCCGCGACGAGCTGCTTGCCAAAGACAAAGCCTTCTTACAGGCGGAAAAAGACAT
TGCCGATCTGGGCGCGCTGTATTCACAAAACTTCCAATTGAAATACAAAAATGTGGAAGAATTCCGCAGCATCCTGCGTT
TGGACAATGCCGACACAACCGGAAACCGCAATACGCTTGTCAGCGGCAGGGGCAGCGTGCTGATCGATCCCGCCACCAAT
ACCCTGATTGTTACCGATACCCGCAGCGTCATCGAAAAATTCCGCAAACTGATTGACGAATTGGACGTACCCGCGCAACA
AGTGATGATTGAGGCGCGTATCGTCGAAGCGGCAGACGGCTTCTCGCGCGATTTGGGCGTTAAATTCGGCGCGACAGGCA
AGAAAAAGCTGAAAAATGATACAAGCGCATTCGGCTGGGGGGTAAACTCCGGCTTCGGCGGCGACGATAAATGGGGGGCC
GAAACCAAAATCAACCTGCCGATTACCGCTGCCGCAAACAGCATTTCGCTGGTGCGCGCGATTTCCTCCGGTGCCTTGAA
TTTGGAATTGTCCGCATCCGAATCGCTTTCAAAAACCAAAACGCTTGCCAATCCGCGCGTGCTGACCCAAAACCGCAAAG
AGGCCAAAATCGAATCTGGTTACGAAATTCCTTTCACCGTAACCTCAATCGCGAACGGCGGCAGCAGCACGAACACGGAA
CTCAAAAAAGCCGTCTTGGGGCTGACCGTTACGCCGAACATCACGCCCGACGGCCAAATCATTATGACCGTCAAAATCAA
CAAGGACTCGCCTGCGCAATGTGCCTCCGGTAATCAGACGATTCTGTGTATTTCGACCAAAAACCTGAATACGCAGGCTA
TGGTTGAAAACGGCGGCACATTGATTGTCGGCGGTATTTATGAAGAAGACAACGGTAATACGCTGACCAAAGTCCCCCTG
TTGGGCGACATCCCCGTTATCGGCAACCTCTTTAAAACACGCGGGAAAAAAACCGACCGCCGCGAACTGCTGATTTTCAT
TACCCCGAGGATTATGGGTACGGCCGGCAACAGCCTGCGCTATTGA

Upstream 100 bases:

>100_bases
TTCCCGTAAAGCAGAACTGCTGTTGAATTCTTCCGACAAAAACACCGAACAAGCGGCAGCACCTGCCGCAGAACAAAATT
AAGAAGAGGATTACTCCATT

Downstream 100 bases:

>100_bases
TGCGTCAAAATAAGGGCATATGTTTTACGGCATATGCCCTTTCTTTATGCTTTTTGCCGCGACCGAAATGCCGTCATTCC
CGCGCAGGCGGGAATCCAAC

Product: type IV pilus assembly protein PilQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 761; Mature: 761

Protein sequence:

>761_residues
MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVL
EYADPLLSKISAAQNSSRARLVLNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFA
GQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKK
QNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQ
QGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATN
TLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGA
ETKINLPITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIPFTVTSIANGGSSTNTE
LKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPL
LGDIPVIGNLFKTRGKKTDRRELLIFITPRIMGTAGNSLRY

Sequences:

>Translated_761_residues
MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVL
EYADPLLSKISAAQNSSRARLVLNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFA
GQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKK
QNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQ
QGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATN
TLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGA
ETKINLPITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIPFTVTSIANGGSSTNTE
LKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPL
LGDIPVIGNLFKTRGKKTDRRELLIFITPRIMGTAGNSLRY
>Mature_761_residues
MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVL
EYADPLLSKISAAQNSSRARLVLNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFA
GQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKK
QNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQ
QGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATN
TLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGA
ETKINLPITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIPFTVTSIANGGSSTNTE
LKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPL
LGDIPVIGNLFKTRGKKTDRRELLIFITPRIMGTAGNSLRY

Specific function: Required for type IV pilus biogenesis and competence. Could function as a pore for exit of the pilus but also as a channel for entry of heme and antimicrobial agents and uptake of transforming DNA [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=422, Percent_Identity=32.9383886255924, Blast_Score=212, Evalue=6e-56,
Organism=Escherichia coli, GI87082242, Length=286, Percent_Identity=29.020979020979, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR011662
- InterPro:   IPR004846
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N; PF07660 STN [H]

EC number: NA

Molecular weight: Translated: 81787; Mature: 81787

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEIVNPTGFVTSS
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCHHCCCCCCEECC
PARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLVLNLNKPGQYNTEVRGNKV
CCEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEECCCEE
WIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAVSVSKPFTPAKQQAAAPFTES
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEECCCCCCHHHHHCCCHHHH
VVSVSAPFSPAKQQAAASAKQQTAAPAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFA
HEEECCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHCCCCCEECCCCCCEEEEEEECCC
GQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKTPVQKVTLKRLNNDTQLIITTAGNW
CCCCCCCCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCE
ELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKES
EEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCC
GMNIVASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAF
CCEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHCCCEEEECCCHHHHHHHHHH
LQAEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATN
HHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCCCCCCEEEECCCCEEECCCCC
TLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKND
EEEEECCHHHHHHHHHHHHHHCCCHHHHEEEHHEEHHCCCCCCCCCEEECCCCHHHHCCC
TSAFGWGVNSGFGGDDKWGAETKINLPITAAANSISLVRAISSGALNLELSASESLSKTK
CCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCEEEEECCCHHHHHHH
TLANPRVLTQNRKEAKIESGYEIPFTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQI
HHCCCEEECCCCCHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHEEEEECCCCCCCCEE
IMTVKINKDSPAQCASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPL
EEEEEECCCCCCHHCCCCCEEEEEEECCCCCEEEEECCCEEEEEEEEECCCCCEEEEECE
LGDIPVIGNLFKTRGKKTDRRELLIFITPRIMGTAGNSLRY
ECCCCHHHHHHHHCCCCCCCCEEEEEECCEEECCCCCCCCC
>Mature Secondary Structure
MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEIVNPTGFVTSS
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCHHCCCCCCEECC
PARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLVLNLNKPGQYNTEVRGNKV
CCEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEECCCEE
WIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAVSVSKPFTPAKQQAAAPFTES
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEECCCCCCHHHHHCCCHHHH
VVSVSAPFSPAKQQAAASAKQQTAAPAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFA
HEEECCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHCCCCCEECCCCCCEEEEEEECCC
GQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKTPVQKVTLKRLNNDTQLIITTAGNW
CCCCCCCCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCE
ELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKES
EEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCC
GMNIVASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAF
CCEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHCCCEEEECCCHHHHHHHHHH
LQAEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATN
HHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCCCCCCEEEECCCCEEECCCCC
TLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKND
EEEEECCHHHHHHHHHHHHHHCCCHHHHEEEHHEEHHCCCCCCCCCEEECCCCHHHHCCC
TSAFGWGVNSGFGGDDKWGAETKINLPITAAANSISLVRAISSGALNLELSASESLSKTK
CCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCEEEEECCCHHHHHHH
TLANPRVLTQNRKEAKIESGYEIPFTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQI
HHCCCEEECCCCCHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHEEEEECCCCCCCCEE
IMTVKINKDSPAQCASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPL
EEEEEECCCCCCHHCCCCCEEEEEEECCCCCEEEEECCCEEEEEEEEECCCCCEEEEECE
LGDIPVIGNLFKTRGKKTDRRELLIFITPRIMGTAGNSLRY
ECCCCHHHHHHHHCCCCCCCCEEEEEECCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]