Click here to switch to the map view.

The map label for this gene is tpiA [H]

Identifier: 161869328

GI number: 161869328

Start: 335803

End: 336576

Strand: Reverse

Name: tpiA [H]

Synonym: NMCC_0334

Alternate gene names: 161869328

Gene position: 336576-335803 (Counterclockwise)

Preceding gene: 161869332

Following gene: 161869327

Centisome position: 15.63

GC content: 55.94

Gene sequence:

>774_bases
ATGTATCACCAAATCGGAATGTGGGATCAAAAATGGGTCATCGGCAACTGGAAAATGAACGGCCGGCTCCAAAACAACAA
TTCGCTCATGCACCGCTTCCGCATCCTCCCCACCGCCGAACGCGTCCTCATCGGACTCGCCGCCCCGACCGTTTACCTGC
TGCAACTGCACAACGCCATGCAAATCGTCTTAAACAACCGCATCCTCACCTGCGCCCAAGACGTGAGCCGCTTCCCCGAC
AACGGCGCGTACACCGGCGAAGTGTCCGCCGAAATGCTCGCCGACACCGGCACAGACATCGTCCTCATCGGACACTCCGA
ACGCAGCCTTTATTTCGGCGAAAAAAACGAAATCCAACGCCGCAAAATGGAAAACGTCCTCAACGTCGGACTCATCCCGT
TATTGTGCGTCGGCGAAAGCCTCGAAGAGCGCGAAGCCGGCAAAGAACACGAAGTCATCGCCCATCAGCTTTCCATCCTG
CAAGGGCTGGATACCAAAAACATCGCCGTCGCCTACGAACCCGTCTGGGCGATCGGCACCGGCAAAGTCGCCACCGTCGA
ACAGATTGCCGATATGCACGCATTCATCTACAAAGAAATCTTGTCTTTGTGCGGAAGCGATGTTAAAATCCGTGTCCTTT
ACGGCGGAAGTGTGAAAGCGGACAACGCGGCCGACATCTTCGCAGTACCTTATGTGGACGGCGCACTCGTCGGCGGCGCG
TCATTGTCGTACGACTCCTTTACCGCCATCATCAGTGCCGCACAAAATGCGTAG

Upstream 100 bases:

>100_bases
CGCCCGCCCCAAGTTGCGAACCCGCCGCATATCCACTAAACTTCACATTGCAACGCGCCACACGCGGCAGACAGAAAAAA
CACGACACGGAGCAAAAAAG

Downstream 100 bases:

>100_bases
AAAAATATGGAAGCCTTCAAAACCCTAATTTGGATTGTTAATATAATTTCCGCTTTGGCCGTCATCGTGTTAGTATTGCT
CCAACACGGCAAAGGCGCGG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLIGLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPD
NGAYTGEVSAEMLADTGTDIVLIGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSVKADNAADIFAVPYVDGALVGGA
SLSYDSFTAIISAAQNA

Sequences:

>Translated_257_residues
MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLIGLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPD
NGAYTGEVSAEMLADTGTDIVLIGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSVKADNAADIFAVPYVDGALVGGA
SLSYDSFTAIISAAQNA
>Mature_257_residues
MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLIGLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPD
NGAYTGEVSAEMLADTGTDIVLIGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSVKADNAADIFAVPYVDGALVGGA
SLSYDSFTAIISAAQNA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=248, Percent_Identity=38.3064516129032, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI226529917, Length=248, Percent_Identity=38.3064516129032, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1790353, Length=253, Percent_Identity=41.501976284585, Blast_Score=170, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17536593, Length=247, Percent_Identity=41.2955465587045, Blast_Score=167, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=38.6178861788618, Blast_Score=150, Evalue=2e-37,
Organism=Drosophila melanogaster, GI28572008, Length=252, Percent_Identity=42.4603174603175, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI28572006, Length=252, Percent_Identity=42.4603174603175, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI28572004, Length=252, Percent_Identity=42.4603174603175, Blast_Score=168, Evalue=4e-42,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 28261; Mature: 28261

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00141 ASP_PROTEASE ; PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLIGLAAPTVYLLQLHNAM
CCCCCCCCCCEEEEEEEEECCEEECCCCEEEHEECCCCHHHHEEEHHHHHHHHHHHHHHH
QIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVLIGHSERSLYFGEKNEIQR
HHHHCCCEEEEHHHHHHCCCCCCEECCCHHHHHHCCCCCEEEEECCCCEEEECCHHHHHH
RKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSILQGLDTKNIAVAYEPVWAIGT
HHHHHHHHCCCHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCEEEECC
GKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSVKADNAADIFAVPYVDGALVGGA
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEECCCCCEEECCC
SLSYDSFTAIISAAQNA
CCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLIGLAAPTVYLLQLHNAM
CCCCCCCCCCEEEEEEEEECCEEECCCCEEEHEECCCCHHHHEEEHHHHHHHHHHHHHHH
QIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVLIGHSERSLYFGEKNEIQR
HHHHCCCEEEEHHHHHHCCCCCCEECCCHHHHHHCCCCCEEEEECCCCEEEECCHHHHHH
RKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSILQGLDTKNIAVAYEPVWAIGT
HHHHHHHHCCCHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCEEEECC
GKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSVKADNAADIFAVPYVDGALVGGA
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEECCCCCEEECCC
SLSYDSFTAIISAAQNA
CCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]