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The map label for this gene is slt [H]

Identifier: 161869262

GI number: 161869262

Start: 277632

End: 279548

Strand: Reverse

Name: slt [H]

Synonym: NMCC_0267

Alternate gene names: 161869262

Gene position: 279548-277632 (Counterclockwise)

Preceding gene: 161869268

Following gene: 161869261

Centisome position: 12.98

GC content: 59.57

Gene sequence:

>1917_bases
ATGTACCTACCCTCTATGAAGCATTCCCTGCCGCTGCTGGCGGCTCTGGTGCTTGCCGCGTGTTCTTCGACAAACACACT
GCCAGCCGGCAAGACCCCGGCAGACAATATAGAAACTGCCGACCTTTCGGCAAGCGTTCCCACCCGCCCTGCCGAACCGG
AAGGAAAAACGCTGGCAGATTACGGCGGCTACCCGTCCGCACTGGATGCAGTGAAACAGAAAAACGATGCCGCCGTCGCC
GCCTATTTGGAAAACGCCGGCGACAGTGCGATGGCGGAAAATGTCCGCAACGAGTGGCTGAAGTCTTTGGGCGCACGCAA
ACAGTGGACGCTGTTTGCACAGGAATATGCCAAACTCGAACCGGCAGGGCGCGCCCAAGAAGTCGAATGCTACGCCGATT
CGAGCCGCAACGACTATACGCGTGCCGCCGAACTGGTCAAAAATACGGGCAAACTGCCTTCGGGCTGCACCAAACTGTTG
GAACAGGCAGCCGCATCCGGCTTGTTGGACGGCAACGACGCCTGGAGGCGCGTGCGCGGACTGCTGGCCGGCCGCCAAAC
CACAGACGCACGCAACCTTGCCGCCGCATTGGGCAGCCCGTTTGACGGCGGTACACAAGGTTCGCGCGAATATGCTCTGT
TGAACGTCATCGGCAAAGAAGCACGCAAATCGCCGAATGCCGCCGCCCTGCTGTCCGAAATGGAAAGCGGTTTAAGCCTC
GAACAACGCAGTTTCGCGTGGGGCGTATTGGGGCATTATCAGTCGCAAAACCTCAATGTGCCTGCCGCCTTGGACTATTA
CGGCAAGGTTGCCGACCGCCGCCAACTGACCGACGACCAAATCGAGTGGTACGCCCGCGCCGCCTTGCGCGCCCGACGTT
GGGACGAGCTGGCCTCCGTTATCTCGCATATGCCCGAAAAACTGCAAAAAAGCCCGACCTGGCTCTACTGGCTGGCACGC
AGCCGCGCCGCAACGGGCAACACGCAAGAGGCGGAAAAACTTTACAAACAGGCGGCAGCGACGGGCAGGAATTTTTATGC
GGTGCTGGCAGGGGAAGAGTTGGGGCGCAAAATCGATACGCGCAACAATGTTCCCGATGCCGACGCAAACAGCGTCCTCC
GTATGGCGGAAGACGGCGCGATTAAGCGTGCGCTGGTACTGTTCCAAAACAGCCGGACCGCCGGCGATGCGAAAATGCGC
CGTCAGGCTCAGGCGGAATGGCGTTTTGCCACACGCGGGTTCGACGAAGACAAGCTGCTGACCGCCGCACAAACCGCGTT
CGACCACGGTTTTTACGATATGGCGGTCAACAGCGCGGAACGCACCGACCGCAAACTCAACTACACCTTGCGCTATATTT
CGCCGTTTAAAGACACGGTAATCCGCCACGCGCAAAATGTTAATGTCGATCCGGCTTGGGTTTACGGCCTGATTCGTCAG
GAAAGCCGCTTCGTTATAGGCGCGCAATCCCGCGTAGGCGCGCAGGGGCTGATGCAGGTTATGCCTGCCACCGCGCGCGA
AATCGCCGGCAAAATCGGTATGGATGCCGCACAACTTTACACCGCCGACGGCAATATCCGTATGGGGACGTGGTATATGG
CGGACACCAAACGCCGCCTGCAAAACAACGAAGTCCTCGCCACCGCAGGCTATAACGCCGGTCCCGGCAGGGCGCGCCGA
TGGCAGGCGGACACGCCCCCTCGAAGGCGCGGTATATGCCGAAACCATCCCGTTTTCCGAAACGCGCGACTATGTCAAAA
AAGTGATGACCAATGCCGCCTACTACGCCGCCCTCTTCGGCGCGCCGCACATCCCGCTCAAACAGCGTATGGGCATTGTT
CCTGCACGCTGACGTACCGATGCCGTCTGAAACCCGCCCGGTCTTTCAGACGGCATTTTTATCCCGAACGGCATTGA

Upstream 100 bases:

>100_bases
GGTGCAGTTTCGGCATATAATTCATGCAAACGCCTGTTCAGACGGCATTTTGTCTTTTCCAACCCTGACCGTTCAGGGTT
CCGATTCTTAAGGAAATCCG

Downstream 100 bases:

>100_bases
CGGCGAACCATAAATATAAGACAATCCGAAAATTGTTTTTCCTGCTTTTTCAAGCAGCTTGACACGGCACAAGCCGACCC
GTTAGGAGGTGATGTTTCCG

Product: soluble lytic murein transglycosylase, putative

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSASVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVA
AYLENAGDSAMAENVRNEWLKSLGARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGKEARKSPNAAALLSEMESGLSL
EQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQLTDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLAR
SRAATGNTQEAEKLYKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADANSVLRMAEDGAIKRALVLFQNSRTAGDAKMR
RQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLRYISPFKDTVIRHAQNVNVDPAWVYGLIRQ
ESRFVIGAQSRVGAQGLMQVMPATAREIAGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARR
WQADTPPRRRGICRNHPVFRNARLCQKSDDQCRLLRRPLRRAAHPAQTAYGHCSCTLTYRCRLKPARSFRRHFYPERH

Sequences:

>Translated_638_residues
MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSASVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVA
AYLENAGDSAMAENVRNEWLKSLGARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGKEARKSPNAAALLSEMESGLSL
EQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQLTDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLAR
SRAATGNTQEAEKLYKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADANSVLRMAEDGAIKRALVLFQNSRTAGDAKMR
RQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLRYISPFKDTVIRHAQNVNVDPAWVYGLIRQ
ESRFVIGAQSRVGAQGLMQVMPATAREIAGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARR
WQADTPPRRRGICRNHPVFRNARLCQKSDDQCRLLRRPLRRAAHPAQTAYGHCSCTLTYRCRLKPARSFRRHFYPERH
>Mature_638_residues
MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSASVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVA
AYLENAGDSAMAENVRNEWLKSLGARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGKEARKSPNAAALLSEMESGLSL
EQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQLTDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLAR
SRAATGNTQEAEKLYKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADANSVLRMAEDGAIKRALVLFQNSRTAGDAKMR
RQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLRYISPFKDTVIRHAQNVNVDPAWVYGLIRQ
ESRFVIGAQSRVGAQGLMQVMPATAREIAGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARR
WQADTPPRRRGICRNHPVFRNARLCQKSDDQCRLLRRPLRRAAHPAQTAYGHCSCTLTYRCRLKPARSFRRHFYPERH

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=455, Percent_Identity=23.2967032967033, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 70816; Mature: 70816

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSASVPTRPAEPEGKTLAD
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECC
YGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKSLGARKQWTLFAQEYAKLE
CCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
PAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLEQAAASGLLDGNDAWRRVRG
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
LLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGKEARKSPNAAALLSEMESGLSL
HHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
EQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQLTDDQIEWYARAALRARRWDELASV
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
ISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKLYKQAAATGRNFYAVLAGEELGRKIDT
HHHHHHHHHCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECHHHCCHHCC
RNNVPDADANSVLRMAEDGAIKRALVLFQNSRTAGDAKMRRQAQAEWRFATRGFDEDKLL
CCCCCCCCHHHHHHHHHCCHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
TAAQTAFDHGFYDMAVNSAERTDRKLNYTLRYISPFKDTVIRHAQNVNVDPAWVYGLIRQ
HHHHHHHHCCHHHHHCCCHHHHCHHHEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHC
ESRFVIGAQSRVGAQGLMQVMPATAREIAGKIGMDAAQLYTADGNIRMGTWYMADTKRRL
CCCEEEECCHHHCHHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCEEEEEEEEHHHHHHH
QNNEVLATAGYNAGPGRARRWQADTPPRRRGICRNHPVFRNARLCQKSDDQCRLLRRPLR
CCCCEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHH
RAAHPAQTAYGHCSCTLTYRCRLKPARSFRRHFYPERH
HHCCCCHHCCCCEEEEEEEEEECCHHHHHHHCCCCCCC
>Mature Secondary Structure
MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSASVPTRPAEPEGKTLAD
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECC
YGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKSLGARKQWTLFAQEYAKLE
CCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
PAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLEQAAASGLLDGNDAWRRVRG
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
LLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGKEARKSPNAAALLSEMESGLSL
HHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
EQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQLTDDQIEWYARAALRARRWDELASV
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
ISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKLYKQAAATGRNFYAVLAGEELGRKIDT
HHHHHHHHHCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECHHHCCHHCC
RNNVPDADANSVLRMAEDGAIKRALVLFQNSRTAGDAKMRRQAQAEWRFATRGFDEDKLL
CCCCCCCCHHHHHHHHHCCHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
TAAQTAFDHGFYDMAVNSAERTDRKLNYTLRYISPFKDTVIRHAQNVNVDPAWVYGLIRQ
HHHHHHHHCCHHHHHCCCHHHHCHHHEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHC
ESRFVIGAQSRVGAQGLMQVMPATAREIAGKIGMDAAQLYTADGNIRMGTWYMADTKRRL
CCCEEEECCHHHCHHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCEEEEEEEEHHHHHHH
QNNEVLATAGYNAGPGRARRWQADTPPRRRGICRNHPVFRNARLCQKSDDQCRLLRRPLR
CCCCEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHH
RAAHPAQTAYGHCSCTLTYRCRLKPARSFRRHFYPERH
HHCCCCHHCCCCEEEEEEEEEECCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]