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The map label for this gene is golB

Identifier: 161869204

GI number: 161869204

Start: 212584

End: 213342

Strand: Reverse

Name: golB

Synonym: NMCC_0208

Alternate gene names: 161869204

Gene position: 213342-212584 (Counterclockwise)

Preceding gene: 161869205

Following gene: 161869203

Centisome position: 9.91

GC content: 59.29

Gene sequence:

>759_bases
ATGAAAATCACCCCCGTCAAAGCCCTAACCGACAACTACATCTGGATGATACAGCACGGCAACCATGCCGTCTGCGTCGA
CCCTTCCGAACCCTCGCCCGTCTTAGAATTCCTCGTCCGCAACCGCCTCATGCTTGCCCAAACATGGGTAACTCACCCCC
ACCCCGACCACGAGGGCGGTGCGGCGGCACTCTGGCGCGGCTACATGGAATCGCCCGTTTACGGCGAATCCGACATCGAA
GCAGCAACCCACACCGTAACCGCCGGCACCCAATTCACCTTCGGCGACGGACAGGTTACCGTTTGGGCAACACCCGGCCA
CACAGACCGCCACACCAGCTACCTTCTCGAAACTTCAGACGGCATACACGTCTTTTGCGGCGACACCCTTTTTTCCGCCG
GCTGCGGACGCGTGTTTACCGGCACAATCGAACAGCTTTACGACAGCTTCCAACGCTTCAACCGCCTGCCTGAAAACACC
CTGTTCTACCCGGCACACGAATACACCGCCGCCAACCTGCGTTTCGCCGCCCATATCGAGCCGGACAACGCCGACATTCA
GACGGCACTGAAAGCGGCGGCGCATACGCCTACCCTGCCCGTTACCCTCGCGCACGAACGCCGCGTCAATCCGTTTTTGC
GCGTCGACCTGCCGCACGTCAGAGACCGCGCCGAGGCATTGAGCGGGAAAACGTTAAACAGCAGCCTCGATACCTTTGTC
GCGCTGCGTGAACTTAAAAACCAATACCGGACGAAATAA

Upstream 100 bases:

>100_bases
GGCTAACCGCCCTACTCAAACCAATGCCGTCTGAAGAATATTTCAGACGGCGTTCCGGCATACCATCCTTTAAACGGTAT
CCGTCCACCGAGGAACACTC

Downstream 100 bases:

>100_bases
AACAATGGGAAAACGCAGCCATTCCTAGGATTTTTATTAAAATCTTAAATAAAATCATACAATCATCGCCAATAGACGAA
AGGACACCGTTGCCTTATAA

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLMLAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIE
AATHTVTAGTQFTFGDGQVTVWATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLPHVRDRAEALSGKTLNSSLDTFV
ALRELKNQYRTK

Sequences:

>Translated_252_residues
MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLMLAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIE
AATHTVTAGTQFTFGDGQVTVWATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLPHVRDRAEALSGKTLNSSLDTFV
ALRELKNQYRTK
>Mature_252_residues
MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLMLAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIE
AATHTVTAGTQFTFGDGQVTVWATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLPHVRDRAEALSGKTLNSSLDTFV
ALRELKNQYRTK

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538322, Length=262, Percent_Identity=37.0229007633588, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI94538320, Length=262, Percent_Identity=37.0229007633588, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI116642887, Length=270, Percent_Identity=34.8148148148148, Blast_Score=134, Evalue=6e-32,
Organism=Homo sapiens, GI21703352, Length=270, Percent_Identity=34.8148148148148, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI14150041, Length=232, Percent_Identity=35.7758620689655, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI46361987, Length=203, Percent_Identity=33.9901477832512, Blast_Score=107, Evalue=9e-24,
Organism=Escherichia coli, GI1786406, Length=261, Percent_Identity=36.7816091954023, Blast_Score=140, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17536925, Length=263, Percent_Identity=32.6996197718631, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320478, Length=264, Percent_Identity=30.3030303030303, Blast_Score=92, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324614, Length=285, Percent_Identity=29.1228070175439, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21356335, Length=264, Percent_Identity=36.3636363636364, Blast_Score=148, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24667711, Length=264, Percent_Identity=36.3636363636364, Blast_Score=147, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24667703, Length=264, Percent_Identity=36.3636363636364, Blast_Score=147, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_NEIM0 (A9M0M3)

Other databases:

- EMBL:   CP000381
- RefSeq:   YP_001598370.1
- ProteinModelPortal:   A9M0M3
- SMR:   A9M0M3
- EnsemblBacteria:   EBNEIT00000011725
- GeneID:   5795520
- GenomeReviews:   CP000381_GR
- KEGG:   nmn:NMCC_0208
- GeneTree:   EBGT00050000020678
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK878330
- BioCyc:   NMEN374833:NMCC_0208-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28155; Mature: 28155

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLMLAQTWVTHPHPDHEGG
CCCCCCEEECCCEEEEEEECCEEEEECCCCCCHHHHHHHHCCCEEEHHHCCCCCCCCCCC
AAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVWATPGHTDRHTSYLLETSD
HHHHHHHHHCCCCCCCCCCHHHHEEEECCCEEEECCCEEEEEECCCCCCCCCEEEEECCC
GIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENTLFYPAHEYTAANLRFAAHIE
CEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEECCEEEEEEEC
PDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLPHVRDRAEALSGKTLNSSLDTFV
CCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHH
ALRELKNQYRTK
HHHHHHHHHCCC
>Mature Secondary Structure
MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLMLAQTWVTHPHPDHEGG
CCCCCCEEECCCEEEEEEECCEEEEECCCCCCHHHHHHHHCCCEEEHHHCCCCCCCCCCC
AAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVWATPGHTDRHTSYLLETSD
HHHHHHHHHCCCCCCCCCCHHHHEEEECCCEEEECCCEEEEEECCCCCCCCCEEEEECCC
GIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENTLFYPAHEYTAANLRFAAHIE
CEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEECCEEEEEEEC
PDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLPHVRDRAEALSGKTLNSSLDTFV
CCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHH
ALRELKNQYRTK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA