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The map label for this gene is truA [H]

Identifier: 161869166

GI number: 161869166

Start: 163337

End: 164134

Strand: Reverse

Name: truA [H]

Synonym: NMCC_0161

Alternate gene names: 161869166

Gene position: 164134-163337 (Counterclockwise)

Preceding gene: 161869167

Following gene: 161869165

Centisome position: 7.62

GC content: 60.15

Gene sequence:

>798_bases
ATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTATGATGGCAGCCGCTTTTACGGCTGGCAGAAACAGGC
TGACGGCGTACCGACCGTTCAGGCGGCATTGGAAACCGCGCTCGCCCAAATAGCAGGGGAAGCGGTTTCCACCACCGTTG
CCGGCAGGACCGATACCGGCGTTCATGCGACTGCCCAAGTCGTTCATTTTGATACTGCCGCCGTCCGTCCGGTTCAGGCA
TGGGTGCGCGGCGTAAATGCCCACCTGCCCGAAGGCATTGCCGTTTTGCACGCCCGACAGGTCGCCCCCGAATTTCACGC
CCGCTTCGACGCATACGGACGGCATTACCGCTACCTGCTCGAATCCGCCCCCGTCCGTTCCCCCCTGCTCAAAAACAGGG
CAGGCTGGACGCATCTCAAACTCGACATCGGGCAGATGCGGCAGGCTGCCGCCTTATTGATCGGCGAACAAGACTTCTCC
AGCTTCCGCGCCGCCGAATGCCAAGCAAAATCCCCCGTCAAAACCATCTACCGCGCCGACCTTACCCAAAGCTCAGGACT
CGTCCGCCTCGATTTGCACGGCAACGCCTTTTTGCACCATATGGTACGCAACATCATGGGCGCGCTCGTCTATGTCGGCA
GCGGCAGACTCAGCGTCGAAGGCTTCGCCGCACTGATTCAAGAACGCAGCCGCCTCAAAGCCCCGCCGACCTTCATGCCC
GACGGACTTTACCTGACCGGCGTCGACTATCCCGAGGCATACGGCATCATCCGCCCCCAAATCCCCGAATGGCTTTAA

Upstream 100 bases:

>100_bases
CCAACTCCCCAAACAACGCCGCTCGCCCTGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACAT
TTATCCGAATCCCAAATCCG

Downstream 100 bases:

>100_bases
AACATTCTTGTCGCGGAGATTTTGAAATCGGACAAACTGTCAGGCAATCTTTTTCAATGTTGACACTACCTCATCAAGGT
AATAACATTGTTATTACATA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQIAGEAVSTTVAGRTDTGVHATAQVVHFDTAAVRPVQA
WVRGVNAHLPEGIAVLHARQVAPEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGQMRQAAALLIGEQDFS
SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLSVEGFAALIQERSRLKAPPTFMP
DGLYLTGVDYPEAYGIIRPQIPEWL

Sequences:

>Translated_265_residues
MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQIAGEAVSTTVAGRTDTGVHATAQVVHFDTAAVRPVQA
WVRGVNAHLPEGIAVLHARQVAPEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGQMRQAAALLIGEQDFS
SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLSVEGFAALIQERSRLKAPPTFMP
DGLYLTGVDYPEAYGIIRPQIPEWL
>Mature_265_residues
MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQIAGEAVSTTVAGRTDTGVHATAQVVHFDTAAVRPVQA
WVRGVNAHLPEGIAVLHARQVAPEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGQMRQAAALLIGEQDFS
SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLSVEGFAALIQERSRLKAPPTFMP
DGLYLTGVDYPEAYGIIRPQIPEWL

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=277, Percent_Identity=30.6859205776173, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1788657, Length=252, Percent_Identity=44.8412698412698, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI71985046, Length=190, Percent_Identity=30.5263157894737, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI116007752, Length=310, Percent_Identity=27.741935483871, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29189; Mature: 29189

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQIAGEAVSTTVAGRTDTG
CCCCCCCEEEEEEEECCCEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
VHATAQVVHFDTAAVRPVQAWVRGVNAHLPEGIAVLHARQVAPEFHARFDAYGRHYRYLL
CEEEEEEEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHH
ESAPVRSPLLKNRAGWTHLKLDIGQMRQAAALLIGEQDFSSFRAAECQAKSPVKTIYRAD
HCCCCCCHHHHCCCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
LTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLSVEGFAALIQERSRLKAPPTFMP
CCCCCCEEEEEECCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCCCCCCC
DGLYLTGVDYPEAYGIIRPQIPEWL
CCEEEECCCCCHHCCCCCCCCCCCC
>Mature Secondary Structure
MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQIAGEAVSTTVAGRTDTG
CCCCCCCEEEEEEEECCCEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
VHATAQVVHFDTAAVRPVQAWVRGVNAHLPEGIAVLHARQVAPEFHARFDAYGRHYRYLL
CEEEEEEEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHH
ESAPVRSPLLKNRAGWTHLKLDIGQMRQAAALLIGEQDFSSFRAAECQAKSPVKTIYRAD
HCCCCCCHHHHCCCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
LTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLSVEGFAALIQERSRLKAPPTFMP
CCCCCCEEEEEECCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCCCCCCC
DGLYLTGVDYPEAYGIIRPQIPEWL
CCEEEECCCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA