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The map label for this gene is thiF [H]

Identifier: 161869131

GI number: 161869131

Start: 124800

End: 125693

Strand: Reverse

Name: thiF [H]

Synonym: NMCC_0125

Alternate gene names: 161869131

Gene position: 125693-124800 (Counterclockwise)

Preceding gene: 161869137

Following gene: 161869124

Centisome position: 5.84

GC content: 60.29

Gene sequence:

>894_bases
ATGCCGCATCCGTGCCAAACCGTTCACACTTTAACCGCCCGTGTCCCGAAATGCCGTCTGAAGTTGAACGCCGCCCGACG
GCAGCGTTACAATCGCCCACAACTGTTTTTTCCGAACATCATCATGACCACGACCGAACACGACAACGACGATGCATTCC
TGCTGCGGTACAGCCGCCACATCCTCTTGGACGAAATCGGCATCGAAGGGCAGCAGAAACTTTCCGCCGCGCATATTTTG
GTCGTCGGCTGCGGCGGTTTGGGTGCCGCCGCCCTGCCCTATCTCGCCGCTTCCGGCATCGGCACGCTGACCATAGCCGA
TTCCGACACGGTCGAGCTGCACAACCTGCAACGCCAAGTCGCATTTGACGAGGGCGATGTCGGCAAGCCCAAAGCCGAAG
CCTTGGCAGACCGCCTGAAACGCATCAACCATAACGTCGATGTCCGCACCGTCAACGAAAAACTCGACGGCTGCCGCCTG
ACCGGTTTGGTTCAAACCGCCGACATCGTTTTAGACTGCTGCGACAACTACGCCACGCGCCAAGCCGTCAACCGTGTCTG
CGTACAAACGAAAACACCGCTGGTTTCAGGGGCGGCGGTACGCTTTGAAGGGCAACTTGCCGTGTACCGTCCCGACTTGC
CCGACTCGCCGTGTTACGCCTGCCTGTTTGACGGCGGATCGGCTTCAGACGGCATCTGTTCTCTCTTCGGCGTGTTCTCG
CCGCTGGTCGGCATCATCGGCAGTACCCAAGCGGCGGAGGCTCTGAAAATCCTGCTGGATGCGGGCGAACCGTCGCACGG
CAGGCTGGCGGTTTACCGTGCCTTGGAAGGGGGCTGGCAATATTTCGACCTGCCGCGCAACCCTGAATGCCCGGTTTGCG
GCACGGCGCGATAA

Upstream 100 bases:

>100_bases
ATTCCGCGTCCGCCGCCAAAGCCGCGTCCTTTGGATTGTTCAGAATATGCAGTTGCATGATTTTTCTCTCATTGCCGTAA
ATACTGTAAATGTACCTCAA

Downstream 100 bases:

>100_bases
ACCCTGCCGCCGTTTCAGACGGCATCCAAACGGATGCGGCGGAACGGTTTTAAAAATTTAAAAATTTACATTTCTTTGCA
AAAAAAAAAAAAAAAAATAA

Product: 5-(beta-hydroxyethyl)thiazole monophosphate synthesis protein

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTTTEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHIL
VVGCGGLGAAALPYLAASGIGTLTIADSDTVELHNLQRQVAFDEGDVGKPKAEALADRLKRINHNVDVRTVNEKLDGCRL
TGLVQTADIVLDCCDNYATRQAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPCYACLFDGGSASDGICSLFGVFS
PLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALEGGWQYFDLPRNPECPVCGTAR

Sequences:

>Translated_297_residues
MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTTTEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHIL
VVGCGGLGAAALPYLAASGIGTLTIADSDTVELHNLQRQVAFDEGDVGKPKAEALADRLKRINHNVDVRTVNEKLDGCRL
TGLVQTADIVLDCCDNYATRQAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPCYACLFDGGSASDGICSLFGVFS
PLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALEGGWQYFDLPRNPECPVCGTAR
>Mature_296_residues
PHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTTTEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILV
VGCGGLGAAALPYLAASGIGTLTIADSDTVELHNLQRQVAFDEGDVGKPKAEALADRLKRINHNVDVRTVNEKLDGCRLT
GLVQTADIVLDCCDNYATRQAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPCYACLFDGGSASDGICSLFGVFSP
LVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALEGGWQYFDLPRNPECPVCGTAR

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=228, Percent_Identity=41.6666666666667, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI150417996, Length=157, Percent_Identity=29.2993630573248, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI23510338, Length=179, Percent_Identity=29.0502793296089, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI23510340, Length=179, Percent_Identity=29.0502793296089, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4885649, Length=151, Percent_Identity=25.1655629139073, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI38045948, Length=165, Percent_Identity=30.9090909090909, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787048, Length=244, Percent_Identity=44.672131147541, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI87082356, Length=246, Percent_Identity=43.4959349593496, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1789177, Length=131, Percent_Identity=33.587786259542, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17540406, Length=244, Percent_Identity=45.0819672131148, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI193203301, Length=158, Percent_Identity=31.6455696202532, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71984476, Length=182, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=251, Percent_Identity=33.0677290836653, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320598, Length=172, Percent_Identity=26.1627906976744, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322639, Length=192, Percent_Identity=28.6458333333333, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24582879, Length=257, Percent_Identity=35.0194552529183, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24660640, Length=152, Percent_Identity=30.9210526315789, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28573937, Length=188, Percent_Identity=29.7872340425532, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24641311, Length=262, Percent_Identity=30.1526717557252, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR012730
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 32197; Mature: 32066

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTTTEHDNDDAFLLRYSRH
CCCCHHHHHHHHHCCCHHEEEECHHHHHHCCCCCEEECCEEEEECCCCCCCEEEEEEHHH
ILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGTLTIADSDTVELHNLQRQV
HHHHHCCCCCHHHHCEEEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
AFDEGDVGKPKAEALADRLKRINHNVDVRTVNEKLDGCRLTGLVQTADIVLDCCDNYATR
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEHHHHHHHHHHHHHCCHHHH
QAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPCYACLFDGGSASDGICSLFGVFS
HHHHHHHHHCCCCCCCCEEEEEECEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
PLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALEGGWQYFDLPRNPECPVCGTAR
HHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
PHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTTTEHDNDDAFLLRYSRH
CCCHHHHHHHHHCCCHHEEEECHHHHHHCCCCCEEECCEEEEECCCCCCCEEEEEEHHH
ILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGTLTIADSDTVELHNLQRQV
HHHHHCCCCCHHHHCEEEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
AFDEGDVGKPKAEALADRLKRINHNVDVRTVNEKLDGCRLTGLVQTADIVLDCCDNYATR
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEHHHHHHHHHHHHHCCHHHH
QAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPCYACLFDGGSASDGICSLFGVFS
HHHHHHHHHCCCCCCCCEEEEEECEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
PLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALEGGWQYFDLPRNPECPVCGTAR
HHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503 [H]