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The map label for this gene is pykA [H]

Identifier: 161869108

GI number: 161869108

Start: 98175

End: 99647

Strand: Reverse

Name: pykA [H]

Synonym: NMCC_0098

Alternate gene names: 161869108

Gene position: 99647-98175 (Counterclockwise)

Preceding gene: 161869114

Following gene: 161869107

Centisome position: 4.63

GC content: 60.29

Gene sequence:

>1473_bases
ATGAACCAGACAAGCCGCGATCTGACCCGCATCAGCCACAACACCAAAATCGTCGCCACCCTTGGGCCGGGCAGCAACAA
CGTAGAACTGTTGGAAGATATGATCCGCGTCGGCGGCCTGAACGTCGTCCGCTTCAACTTCAGCCACGGCACGCCCGAAT
TCCATCAGGAAAACGCCCGCATCGTGCGCGAGGCGGCAAAACGCGCCGGACAGGAAATCGCCATCATTGCCGACCTGCAA
GGGCCGAAAATCCGCGTGGGCAAAATCGCCGGCGGCGGCATTGAATTGAACAAAGGCGAAACGCTGGTACTGGATGCCGC
GCTCGAAGGCGAAGGCACGCGCGAGGCGGTCGGTTTGGACTACCGCGACCTGCCCGACGACGTTGCCGCAGGCGACGTAT
TGTGGCTGGACGACGGCCTGCTGACCCTGACCGTGGAATCCGTCGAAGGCAGCAGGATTATCACAAGGGTGGAAAACAGC
CACGTCCTGAAAAGCAACAAGGGCATCAACAAACGCGGCGGCGGTCTGTCCGCAGGCGCGTTGACCGAAAAAGACTTCCG
CGACCTGAAAACCGCGATTGCCATCGGTTGCGACTACCTCGCCATCAGCTTTGTGAAATCCGCCGAAGATTTGCATATCG
CGCGCGCCAAAGTCGAAGAAGAAATGAAGGGCAGCACGGCCGTGCGCCCCGGTTTGGTTTCCAAAATCGAACGCGTGGAA
GCGATTGAAAACTTGGACGAAATCATCCTTGCCGGCGACGGCATTATGGTTGCGCGCGGCGACTTGGCGGTCGAAGTCGG
ACACGCCGCCGTCCCCGCCCTGCAAAAACGGATGATCCGCCGCGCCCGCGAGTTGCGCCGCTTCAGCATTACGGCGACGC
AAATGATGGAATCGATGATTACCAACCCCGTGCCGACCCGCGCGGAAGTCAGCGATGTGGCAAACGCGGTATTGGACGGC
ACCGATGCGGTGATGTGTTCCGCCGAAACCGCCGTCGGCGCGTATCCGTTTGAAACCGTCAGCCAAATGGCAATTATCTG
CGCGGCTGCGGAAAAAGAGCAGGATTCGCTCAACGGCGTTGCCGAACAGGCGGAGTATCCCGAAGCGGTCAGCACCAACC
TGGCGGTTGCCGGCGGCGCGGTCAGCGTGGCGCGCGCGGTTCACGCCAAAGCCATCGTCGCCCTGACCGAAAGCGGTTCG
ACCGCCTTTGAAATCAGCCGCCACAACATCACCCTGCCGATTTTCGCGCTGACCCCGAGCGTTTCCGCCCAACGCCGTAT
GGCGATGTACCGGGGCGTGCGCCCGCTGATTTTGGCAACCAGCACCGACCACGACACGGCGTTAAACGAAGTCGAAACCA
TGCTGGTGGAACACAACATCCTGCATTCCGGCGACCAATACATCATTACCAGCGGCTCGCAAATGCGCGAATCCGGTTCG
ACCAATACGCTGGAAGTGCTGCGCGTCAAATAA

Upstream 100 bases:

>100_bases
CCGCCGCGTTCATCAAATGGACATCAAACCGGTTCCAAATTTCTGTAATTTTGTAACAAAATACCGCAAAACACCCGATT
GAGACCAAAAGGACTTTCAT

Downstream 100 bases:

>100_bases
TCCGCCCTGAGTGGAAAATGCCGTCTGAAGTCGATGCCCGAGGCTTCAGACGGCATTTTTTGCGGCGGCGCGGCGGTTCG
GGCAAACTCTGAAAAAATTG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVRFNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQ
GPKIRVGKIAGGGIELNKGETLVLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKVEEEMKGSTAVRPGLVSKIERVE
AIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQKRMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDG
TDAVMCSAETAVGAYPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAIVALTESGS
TAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNEVETMLVEHNILHSGDQYIITSGSQMRESGS
TNTLEVLRVK

Sequences:

>Translated_490_residues
MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVRFNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQ
GPKIRVGKIAGGGIELNKGETLVLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKVEEEMKGSTAVRPGLVSKIERVE
AIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQKRMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDG
TDAVMCSAETAVGAYPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAIVALTESGS
TAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNEVETMLVEHNILHSGDQYIITSGSQMRESGS
TNTLEVLRVK
>Mature_490_residues
MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVRFNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQ
GPKIRVGKIAGGGIELNKGETLVLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKVEEEMKGSTAVRPGLVSKIERVE
AIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQKRMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDG
TDAVMCSAETAVGAYPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAIVALTESGS
TAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNEVETMLVEHNILHSGDQYIITSGSQMRESGS
TNTLEVLRVK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=497, Percent_Identity=38.2293762575453, Blast_Score=292, Evalue=6e-79,
Organism=Homo sapiens, GI33286420, Length=497, Percent_Identity=38.2293762575453, Blast_Score=292, Evalue=6e-79,
Organism=Homo sapiens, GI33286418, Length=496, Percent_Identity=38.1048387096774, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI10835121, Length=503, Percent_Identity=37.9721669980119, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI32967597, Length=503, Percent_Identity=37.9721669980119, Blast_Score=275, Evalue=7e-74,
Organism=Homo sapiens, GI310128732, Length=294, Percent_Identity=38.4353741496599, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI310128730, Length=294, Percent_Identity=38.4353741496599, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI310128736, Length=240, Percent_Identity=37.0833333333333, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI310128734, Length=240, Percent_Identity=37.0833333333333, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI310128738, Length=220, Percent_Identity=35.9090909090909, Blast_Score=122, Evalue=8e-28,
Organism=Escherichia coli, GI1788160, Length=488, Percent_Identity=49.1803278688525, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1787965, Length=473, Percent_Identity=37.4207188160677, Blast_Score=290, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI17544584, Length=489, Percent_Identity=35.9918200408998, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17506829, Length=498, Percent_Identity=34.5381526104418, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI71984413, Length=498, Percent_Identity=34.5381526104418, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17506831, Length=498, Percent_Identity=34.5381526104418, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI71984406, Length=498, Percent_Identity=34.5381526104418, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=35.523613963039, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6324923, Length=437, Percent_Identity=35.2402745995423, Blast_Score=233, Evalue=7e-62,
Organism=Drosophila melanogaster, GI24648964, Length=495, Percent_Identity=38.989898989899, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI28571814, Length=495, Percent_Identity=38.989898989899, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24648966, Length=419, Percent_Identity=37.708830548926, Blast_Score=238, Evalue=6e-63,
Organism=Drosophila melanogaster, GI24581235, Length=488, Percent_Identity=30.7377049180328, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24646914, Length=285, Percent_Identity=37.5438596491228, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52450; Mature: 52450

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVRFNFSHGTPEFHQENAR
CCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHH
IVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETLVLDAALEGEGTREAVGLD
HHHHHHHHCCCEEEEEEECCCCEEEEEEEECCCEEECCCCEEEEEEEECCCCCCHHCCCC
YRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENSHVLKSNKGINKRGGGLSAGA
CCCCCCCCCCCCEEEEECCEEEEEEECCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCC
LTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKVEEEMKGSTAVRPGLVSKIERVE
CCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQKRMIRRARELRRFSITATQMMESMI
HHHCHHHEEEECCCEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGAYPFETVSQMAIICAAAEKEQDSLNGV
CCCCCCCHHHHHHHHHHHCCCCEEEEECCHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
AEQAEYPEAVSTNLAVAGGAVSVARAVHAKAIVALTESGSTAFEISRHNITLPIFALTPS
HHHHCCCHHHCCCEEEECCHHHHHHHHHHEEEEEEECCCCEEEEEECCEEEEEEEEECCC
VSAQRRMAMYRGVRPLILATSTDHDTALNEVETMLVEHNILHSGDQYIITSGSQMRESGS
CHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHCCC
TNTLEVLRVK
CCEEEEEEEC
>Mature Secondary Structure
MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVRFNFSHGTPEFHQENAR
CCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHH
IVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETLVLDAALEGEGTREAVGLD
HHHHHHHHCCCEEEEEEECCCCEEEEEEEECCCEEECCCCEEEEEEEECCCCCCHHCCCC
YRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENSHVLKSNKGINKRGGGLSAGA
CCCCCCCCCCCCEEEEECCEEEEEEECCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCC
LTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKVEEEMKGSTAVRPGLVSKIERVE
CCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQKRMIRRARELRRFSITATQMMESMI
HHHCHHHEEEECCCEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGAYPFETVSQMAIICAAAEKEQDSLNGV
CCCCCCCHHHHHHHHHHHCCCCEEEEECCHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
AEQAEYPEAVSTNLAVAGGAVSVARAVHAKAIVALTESGSTAFEISRHNITLPIFALTPS
HHHHCCCHHHCCCEEEECCHHHHHHHHHHEEEEEEECCCCEEEEEECCEEEEEEEEECCC
VSAQRRMAMYRGVRPLILATSTDHDTALNEVETMLVEHNILHSGDQYIITSGSQMRESGS
CHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHCCC
TNTLEVLRVK
CCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]