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The map label for this gene is mscS [H]

Identifier: 161869060

GI number: 161869060

Start: 37561

End: 38409

Strand: Direct

Name: mscS [H]

Synonym: NMCC_0043

Alternate gene names: 161869060

Gene position: 37561-38409 (Clockwise)

Preceding gene: 161869059

Following gene: 161869061

Centisome position: 1.74

GC content: 53.47

Gene sequence:

>849_bases
ATGGACTTCAAACAATTTGATTTTTTACACCTGATCAGTGTTTCCGGTTGGGAGCATCTGGCTGAAAAGGCGTGGGCGTT
CGGGCTGAACCTTGCCGCCGCGCTGCTTATTTTTTTGGTCGGAAAATGGGCGGCGAAACGCATTGTCGCTGTGATGAGGG
CGGCGATGACGCGCGCGCAGGTCGATGCCACGCTGATTAGTTTTTTGTGTAATGTTGCCAATATCGGCTTATTGATTTTG
GTGATTATTGCCGCATTGGGCAGATTGGGCGTTTCCACAACATCCGTAACCGCCTTAATCGGCGGCGCGGGTTTGGCGGT
GGCGTTGTCCTTGAAAGACCAGCTGTCCAATTTTGCCGCCGGCGCGCTGATTATCCTGTTCCGCCCGTTCAAAGTCGGCG
ATTTTATCCGCGTCGGCGGTTTTGAAGGATATGTCCGAGAGATTAAAATGGTGCAGACTTCTTTGCGGACGACCGACAAC
GAAGAAGTCGTGCTGCCCAACAGCGTGGTGATGGGCAACAGCATCGTCAACCGTTCCACACTGCCGCTGTGCCGCGCCCA
AGTGATAGTCGGCGTCGATTACAACTGCGATTTGAAAGTGGCGAAAGAGGCGGTGTTGAAAGCCGCCGTCGAACACCCCT
TGAGCGTTCAAAACGAAGAGCGGCAGGCCGCCGCCTACATCACCGCCTTGGGCGACAATGCCATCGAAATCACATTATGG
GCTTGGGCAAACGAAGCAGACCGCTGGACGCTGCAATGCGACTTGAACGAACAAGTGGTCGAAAACCTCCGCAAAGTCAA
TATCAACATCCCGTTCCCGCAACGCGACATACACATCATCAATTCTTAA

Upstream 100 bases:

>100_bases
GAAATAAAACAAACATACCGCCCCGCAGGGCTTCATACGGCATTTTTACACCTGTGCCGATTACGCCGCACGGGGCGGAT
GTTCGATCAAGAGGAAAACA

Downstream 100 bases:

>100_bases
ACGCCGTCTGAAAGAGGAGTGGGAAATGGACGCGCTGCACACCATCGCCCGAAACCTGACGAAAAAACGTCAAACCGTAA
GCTGTGCCGAATCCTGCACG

Product: integral membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWAAKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLIL
VIIAALGRLGVSTTSVTALIGGAGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQNEERQAAAYITALGDNAIEITLW
AWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRDIHIINS

Sequences:

>Translated_282_residues
MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWAAKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLIL
VIIAALGRLGVSTTSVTALIGGAGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQNEERQAAAYITALGDNAIEITLW
AWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRDIHIINS
>Mature_282_residues
MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWAAKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLIL
VIIAALGRLGVSTTSVTALIGGAGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQNEERQAAAYITALGDNAIEITLW
AWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRDIHIINS

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=253, Percent_Identity=33.596837944664, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1786670, Length=202, Percent_Identity=29.7029702970297, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787591, Length=227, Percent_Identity=25.1101321585903, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI2367355, Length=244, Percent_Identity=20.0819672131148, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 30796; Mature: 30796

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWAAKRIVAVMRAAMTRAQ
CCCCHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGGAGLAVALSLKDQLSNFAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHC
GALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDNEEVVLPNSVVMGNSIVNRST
CEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCEEECCCEEECCCCCCCCC
LPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQNEERQAAAYITALGDNAIEITLW
CCHHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHEEEEECCCEEEEEEE
AWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRDIHIINS
EECCCCCCEEEEECCCHHHHHHHHEEEEECCCCCCCEEEECC
>Mature Secondary Structure
MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWAAKRIVAVMRAAMTRAQ
CCCCHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGGAGLAVALSLKDQLSNFAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHC
GALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDNEEVVLPNSVVMGNSIVNRST
CEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCEEECCCEEECCCCCCCCC
LPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQNEERQAAAYITALGDNAIEITLW
CCHHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHEEEEECCCEEEEEEE
AWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRDIHIINS
EECCCCCCEEEEECCCHHHHHHHHEEEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]