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The map label for this gene is hprA [H]

Identifier: 161869047

GI number: 161869047

Start: 22191

End: 23144

Strand: Direct

Name: hprA [H]

Synonym: NMCC_0030

Alternate gene names: 161869047

Gene position: 22191-23144 (Clockwise)

Preceding gene: 161869039

Following gene: 161869050

Centisome position: 1.03

GC content: 54.72

Gene sequence:

>954_bases
ATGAATCACAAAAAGATAGTCGTTCTGGATGCGGATACTTTGCCCGGCCGGGTTTTTCATTTTGATTTTCCGCACGAGCT
TGCGGTTTACGGTACGACAGGTGCGGATGAAACGGCAGAACGGGTGCGCGACGCACATATTGTCATTACCAATAAGGTAA
TGATTTCCGCCGACATTATTGCGGCTAATCCGCAGTTGGAGCTGATTGCCGTCAGTGCGACCGGTGTGAACAATGTCGAT
ATTGGGGCGGCAAAGGCGGCGGGCGTTGCGGTATGCAATGTCCGCGCATACGGAAACGAATCGGTTGCGGAACACGCCTT
TATGCTGATGATTGCCTTGATGCGGAATTTGCCCGCCTATCAGCGTGATGTTGCGGCAGGATTATGGGAGAAGTCGCCAT
TTTTCTGTCATTACGGCGCGCCGATTCGGGATTTGAACGGTAAAACGCTGGCGGTTTTCGGACGCGGCAATATCGGGAGG
ACGCTGGCCGGATACGCGCAGGCATTCGGTATGGGGGTGGTGTTTGCCGAACACAAACACGCGCCCGACGTGCGCGAAGG
CTATGTTTCCTTTGAAGATGCGGTACGGACTGCCGACGTGTTGTCGCTGCACTGTCCGCTGAACGCCCAAACTGAAAATA
TGATAGGCGAAAACGAATTGCGGCAGATGAAGCCCGGTGCGGTTTTGATTAATTGCGGGCGCGGCGGGCTGGTGGATGAA
AACGCGCTGCTTGCCGCACTCAAATACGGGCAGATCGGCGGGGCGGGTGTCGATGTTTTGACGGAGGAGCCGCCAAGGGG
CGGCAATCCCCTGCTGAATGCACGACTTCCCAATCTGATTGTTACGCCGCATACCGCGTGGGCAAGTCGTGAGGCTTTGG
ACAGGCTGTTTGATATATTGTTGGCGAACATTCACGCCTTTGTGAAAGGAGAGGCACAAAATCGGGTGGTTTGA

Upstream 100 bases:

>100_bases
TTACGGAGAGATGATAAAGATTATCATCAGGCCGTGCCGGTTTAAATTTGCTATTTACTGTTAGTGTAAATAAATCAATT
TTAAAGTAAGGATAGAAATG

Downstream 100 bases:

>100_bases
GCTTGTCGGGATTATGGGAAAACAATGCCGTCTGAAGGCCTCAAGGGTCCAGACGGCATTGTTTTTGATATTTCTACTTA
ACCGACTTTGTCGCCCGGTT

Product: glycerate dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRDAHIVITNKVMISADIIAANPQLELIAVSATGVNNVD
IGAAKAAGVAVCNVRAYGNESVAEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
TLAGYAQAFGMGVVFAEHKHAPDVREGYVSFEDAVRTADVLSLHCPLNAQTENMIGENELRQMKPGAVLINCGRGGLVDE
NALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARLPNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV

Sequences:

>Translated_317_residues
MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRDAHIVITNKVMISADIIAANPQLELIAVSATGVNNVD
IGAAKAAGVAVCNVRAYGNESVAEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
TLAGYAQAFGMGVVFAEHKHAPDVREGYVSFEDAVRTADVLSLHCPLNAQTENMIGENELRQMKPGAVLINCGRGGLVDE
NALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARLPNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV
>Mature_317_residues
MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRDAHIVITNKVMISADIIAANPQLELIAVSATGVNNVD
IGAAKAAGVAVCNVRAYGNESVAEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
TLAGYAQAFGMGVVFAEHKHAPDVREGYVSFEDAVRTADVLSLHCPLNAQTENMIGENELRQMKPGAVLINCGRGGLVDE
NALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARLPNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=321, Percent_Identity=30.8411214953271, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI145580578, Length=252, Percent_Identity=36.9047619047619, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI4557499, Length=252, Percent_Identity=36.9047619047619, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI145580575, Length=270, Percent_Identity=35.1851851851852, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4557497, Length=234, Percent_Identity=35.042735042735, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI61743967, Length=234, Percent_Identity=35.042735042735, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI6912396, Length=258, Percent_Identity=27.5193798449612, Blast_Score=101, Evalue=8e-22,
Organism=Escherichia coli, GI1789279, Length=271, Percent_Identity=32.1033210332103, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87082289, Length=298, Percent_Identity=28.8590604026846, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787645, Length=262, Percent_Identity=34.3511450381679, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1788660, Length=225, Percent_Identity=26.6666666666667, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=271, Percent_Identity=32.8413284132841, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25147481, Length=240, Percent_Identity=30, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6320925, Length=270, Percent_Identity=30.7407407407407, Blast_Score=126, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6322116, Length=270, Percent_Identity=31.4814814814815, Blast_Score=126, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6324055, Length=243, Percent_Identity=29.2181069958848, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324964, Length=251, Percent_Identity=26.2948207171315, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6325144, Length=302, Percent_Identity=23.841059602649, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321253, Length=284, Percent_Identity=25, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24646446, Length=273, Percent_Identity=33.3333333333333, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24646448, Length=273, Percent_Identity=33.3333333333333, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24646452, Length=273, Percent_Identity=33.3333333333333, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24646450, Length=273, Percent_Identity=33.3333333333333, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI62472511, Length=273, Percent_Identity=33.3333333333333, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI28574286, Length=262, Percent_Identity=31.6793893129771, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19921140, Length=309, Percent_Identity=28.8025889967638, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24585516, Length=228, Percent_Identity=29.8245614035088, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28571528, Length=258, Percent_Identity=30.2325581395349, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585514, Length=257, Percent_Identity=27.6264591439689, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI28574282, Length=257, Percent_Identity=27.6264591439689, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI45551003, Length=257, Percent_Identity=27.6264591439689, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI45552429, Length=257, Percent_Identity=27.6264591439689, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI28574284, Length=257, Percent_Identity=27.6264591439689, Blast_Score=95, Evalue=6e-20,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 34065; Mature: 34065

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRDAHIVITNKVMISADII
CCCCEEEEEECCCCCCEEEEECCCCCEEEEECCCCHHHHHHHCCEEEEEEEEEEEEEEEE
AANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESVAEHAFMLMIALMRNLPAY
ECCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCHH
QRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGRTLAGYAQAFGMGVVFAEHKH
HHHHHHHHCCCCCEEEECCCCEEECCCCEEEEEECCCCCHHHHHHHHHHCCEEEEECCCC
APDVREGYVSFEDAVRTADVLSLHCPLNAQTENMIGENELRQMKPGAVLINCGRGGLVDE
CCCHHHHCCCHHHHHHHEEEEEEECCCCCCHHHCCCHHHHHHCCCCEEEEECCCCCCCCC
NALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARLPNLIVTPHTAWASREALDRLFDIL
CHHEEEEECCCCCCCCEEEEECCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHH
LANIHAFVKGEAQNRVV
HHHHHHEEECCCCCCCC
>Mature Secondary Structure
MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRDAHIVITNKVMISADII
CCCCEEEEEECCCCCCEEEEECCCCCEEEEECCCCHHHHHHHCCEEEEEEEEEEEEEEEE
AANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESVAEHAFMLMIALMRNLPAY
ECCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCHH
QRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGRTLAGYAQAFGMGVVFAEHKH
HHHHHHHHCCCCCEEEECCCCEEECCCCEEEEEECCCCCHHHHHHHHHHCCEEEEECCCC
APDVREGYVSFEDAVRTADVLSLHCPLNAQTENMIGENELRQMKPGAVLINCGRGGLVDE
CCCHHHHCCCHHHHHHHEEEEEEECCCCCCHHHCCCHHHHHHCCCCEEEEECCCCCCCCC
NALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARLPNLIVTPHTAWASREALDRLFDIL
CHHEEEEECCCCCCCCEEEEECCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHH
LANIHAFVKGEAQNRVV
HHHHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]