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The map label for this gene is pgk [H]

Identifier: 161869028

GI number: 161869028

Start: 6205

End: 7383

Strand: Reverse

Name: pgk [H]

Synonym: NMCC_0010

Alternate gene names: 161869028

Gene position: 7383-6205 (Counterclockwise)

Preceding gene: 161869029

Following gene: 161869023

Centisome position: 0.34

GC content: 57.68

Gene sequence:

>1179_bases
ATGGCATTTTTAAAACTGACCGAACAAAACGTGCAGGGCAAAACCGTCCTCATCCGCGCCGATATGAACGTGCCGTTCAA
AGACGGCAAAATCAGCGACGACACCCGTATCCGCGCTTCGCTCGCGTCCGTCAAATACTGCTTGGACAACGGCGCGTCCG
TTATCGTGATGACCCACTTGGGCCGCCCGACTGAAGGCGAGTTCCACCCCGAAGACGACGTTGCGCCCGTTGCCGCGCAT
TTGGGCGGTCTGTTGGGCAAAGACGTGAAAGTATTGAACGACTGGCGCGAAAACAAACCGGCTTTGAACGCGGGCGATGT
CGTGATGCTGCAAAACGTGCGTATCAACAAAGGCGAGAAGAAAAACGATTTGGAACTGGGCAAAGCCTACGCTTCTTTGT
GCGACGTGTTCGTCAATGACGCGTTCGGCACCGCCCACCGCGCCCAAGCCTCGACCGAAGCCGTCGCCCAAGCCGCGCCC
GTTGCCTGCGCCGGCGTATTGATGGCGGGCGAACTCGACGCTTTGGGCAAAGCCTTGAAACAGCCTGCACGCCCGATGGT
TGCCATCGTTGCCGGCAGCAAAGTGTCCACCAAGCTGACCATCCTCGAATCGCTGGCGGACAAAGTCGACCAACTCATCG
TCGGCGGCGGTATCGCCAACACCTTCCTGTTGGCGGAAGGTAAAGCCATCGGCAAATCTTTGGCGGAACACGATTTGGTG
GAAGAATCCAAAAAAATTATGGCAAAAATGGCGGCCAAAGGCGGTTCTGTGCCGCTGCCGACCGATGTCGTCGTTGCCAA
AGCCTTTGCCGCCGATGCCGAAGCGGTGGTAAAAGACATTGCCGACGTTGCCGAAGACGAGATGATTTTGGACATCGGCC
CGAAATCTGCCGCCGCGCTTGCCGATTTGCTTAAAGCCGCCGACACGGTCGTTTGGAACGGACCGGTCGGCGTGTTCGAG
TTTGACCAGTTCGCAGGCGGTACGAAAGCCCTTACCGAAGCCATTGCCCAAAGCAAAGCGTTCTCCATTGCTGGCGGCGG
CGACACGCTGGCGGCGATTGCCAAATTCGGCGTTACCGACCAAATCGGCTACATCTCCACCGGAGGCGGCGCGTTCCTTG
AGTTCTTGGAAGGTAAAGAGTTGCCTGCCGTAGCCGCTTTGGAAAAACGCGGCGAGTAA

Upstream 100 bases:

>100_bases
ATCTGTTCCAACCACTAATACACTAGTGTTGCACTTGAAATCCGAATCCAAGGGCATCAATTGTTTCTGTATCGCAAACA
CTGATTGAAAAGGACTGATT

Downstream 100 bases:

>100_bases
ACGGTTTGATATAAAAAAATGCCGTCTGAAACGTTGTGCTGTTTCAGACGGCATTTTTGTGTGGCGGTTATTGTGCTTTG
GCATTCCATTCTGCCGGTGT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASVKYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAH
LGGLLGKDVKVLNDWRENKPALNAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGIANTFLLAEGKAIGKSLAEHDLV
EESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAVVKDIADVAEDEMILDIGPKSAAALADLLKAADTVVWNGPVGVFE
FDQFAGGTKALTEAIAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGE

Sequences:

>Translated_392_residues
MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASVKYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAH
LGGLLGKDVKVLNDWRENKPALNAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGIANTFLLAEGKAIGKSLAEHDLV
EESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAVVKDIADVAEDEMILDIGPKSAAALADLLKAADTVVWNGPVGVFE
FDQFAGGTKALTEAIAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGE
>Mature_391_residues
AFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASVKYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHL
GGLLGKDVKVLNDWRENKPALNAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAPV
ACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGIANTFLLAEGKAIGKSLAEHDLVE
ESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAVVKDIADVAEDEMILDIGPKSAAALADLLKAADTVVWNGPVGVFEF
DQFAGGTKALTEAIAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGE

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=410, Percent_Identity=41.9512195121951, Blast_Score=281, Evalue=7e-76,
Organism=Homo sapiens, GI4505763, Length=410, Percent_Identity=41.219512195122, Blast_Score=276, Evalue=2e-74,
Organism=Escherichia coli, GI1789294, Length=392, Percent_Identity=58.6734693877551, Blast_Score=449, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17508823, Length=412, Percent_Identity=41.0194174757282, Blast_Score=285, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6319857, Length=414, Percent_Identity=43.2367149758454, Blast_Score=288, Evalue=1e-78,
Organism=Drosophila melanogaster, GI17136394, Length=425, Percent_Identity=41.6470588235294, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24581203, Length=412, Percent_Identity=38.5922330097087, Blast_Score=242, Evalue=3e-64,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 40734; Mature: 40603

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASVKYCLDNGASVIVMTHL
CCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEC
GRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPALNAGDVVMLQNVRINKGEK
CCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCC
KNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAPVACAGVLMAGELDALGKALK
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHH
QPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGIANTFLLAEGKAIGKSLAEHDLV
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCHHHHHHHHHHHHH
EESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAVVKDIADVAEDEMILDIGPKSAAAL
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCHHHHH
ADLLKAADTVVWNGPVGVFEFDQFAGGTKALTEAIAQSKAFSIAGGGDTLAAIAKFGVTD
HHHHHHHCCEEECCCCCEEECHHHCCCHHHHHHHHHHHCCEEEECCCHHHHHHHHCCCCC
QIGYISTGGGAFLEFLEGKELPAVAALEKRGE
CCCEEECCHHHHHHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
AFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASVKYCLDNGASVIVMTHL
CEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEC
GRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPALNAGDVVMLQNVRINKGEK
CCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCC
KNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAPVACAGVLMAGELDALGKALK
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHH
QPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGIANTFLLAEGKAIGKSLAEHDLV
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCHHHHHHHHHHHHH
EESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAVVKDIADVAEDEMILDIGPKSAAAL
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCHHHHH
ADLLKAADTVVWNGPVGVFEFDQFAGGTKALTEAIAQSKAFSIAGGGDTLAAIAKFGVTD
HHHHHHHCCEEECCCCCEEECHHHCCCHHHHHHHHHHHCCEEEECCCHHHHHHHHCCCCC
QIGYISTGGGAFLEFLEGKELPAVAALEKRGE
CCCEEECCHHHHHHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA