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The map label for this gene is metK [H]

Identifier: 161526247

GI number: 161526247

Start: 3360541

End: 3361764

Strand: Reverse

Name: metK [H]

Synonym: Bmul_3078

Alternate gene names: 161526247

Gene position: 3361764-3360541 (Counterclockwise)

Preceding gene: 161526258

Following gene: 161526245

Centisome position: 97.49

GC content: 65.28

Gene sequence:

>1224_bases
GTGAAACCAACCGTAACCACACAGGAGCCTGAAAAAGTGGCAAACGATTATCTCTTCACGTCCGAATCCGTCTCCGAAGG
TCATCCGGACAAGGTCGCCGACCAGATCTCGGACGCGATTCTCGACGCCATCCTCGAACAGGACAAATACTCCCGCGTTG
CCGCCGAAACGCTGTGCAACACGGGTCTCGTCGTCCTGGCCGGCGAAATCACCACGACGGCCAACATCGACTACATCCAG
ATCGCGCGCGACACGATCAAGCGCATCGGCTACGACAACACCGACTACGGCATCGACTACAAGGGCTGCGCGGTGCTCGT
CGCGTACGACAAGCAGTCGCCGGACATCGCGCAGGGCGTCGATCGCGCGCACGACGACAACCTCGATCAGGGCGCGGGCG
ACCAGGGCCTGATGTTCGGCTATGCCTGCGACGAAACGCCGGAACTGATGCCGCTGCCGATTTATCTGTCGCACCGCCTC
GTCGAGCGCCAGGCGAGCCTGCGCCGCGACGGCCGTCTGCCCTGGCTGCGCCCGGACGCGAAGTCGCAGGTCACGATCCG
CTATGTCGACGGCAAGCCCGATTCGATCGACACCGTCGTGCTGTCCACGCAGCACGCGCCGGACATCGAGCTGCCGGCGC
TGCGCGAAGCCGTGATCGAGGAAATCATCAAGCCGACGCTGCCGGCAGACCTGATCAAGGGCGACATCAAGTTCCTCGTG
AACCCGACCGGCCGGTTCGTGATCGGCGGCCCGCAGGGCGACTGCGGTCTGACGGGCCGCAAGATCATCGTCGACACCTA
CGGCGGCGCGGCTCCGCACGGCGGCGGCGCGTTCTCGGGCAAGGATCCGTCGAAGGTCGACCGTTCGGCTGCGTATGCGG
GCCGTTACGTCGCGAAGAACATCGTCGCCGCGGGCCTCGCGTCGCGCGCGCTGATCCAGGTGTCGTACGCGATCGGCGTG
GCCGAGCCGACCTCGGTGATGGTCAACACGTTCGGCACGGGCCGCGTGTCGGATGCGGTGATTACGAAGCTCGTGCGCGA
GCATTTCGACCTGCGTCCGAAGGGCATCATCAAGATGCTCGATCTGCTGCGCCCGATCTACGAGAAGACGGCCGCTTACG
GCCACTTCGGCCGCGAAGAGCCGGAATTCTCGTGGGAAGCGACCGACAAGGCGCTCGCGCTGGCCGAAGCGGCCGGTGTC
GAGCCGACGGCACGCGTCGCGTAA

Upstream 100 bases:

>100_bases
CTGACAACTTGCGGGGCGAAGCCGGTTGGCGCGATGCGCGCTGCCCGGCACTGGTAATCCGCTAAAGCGTCGCCGCTTCA
GGCCGAAAGAAGCTGGCTAC

Downstream 100 bases:

>100_bases
GCGCGGTTCGCCCCAAAACGAAAAAACCCCGGCATGCCGGGGTTTTTTCTTGCGCGTCGCCCGTGCACGGGCGCGGATGC
GGCGATGCCGCTCGGTTCAG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MKPTVTTQEPEKVANDYLFTSESVSEGHPDKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQ
IARDTIKRIGYDNTDYGIDYKGCAVLVAYDKQSPDIAQGVDRAHDDNLDQGAGDQGLMFGYACDETPELMPLPIYLSHRL
VERQASLRRDGRLPWLRPDAKSQVTIRYVDGKPDSIDTVVLSTQHAPDIELPALREAVIEEIIKPTLPADLIKGDIKFLV
NPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGV
AEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLRPIYEKTAAYGHFGREEPEFSWEATDKALALAEAAGV
EPTARVA

Sequences:

>Translated_407_residues
MKPTVTTQEPEKVANDYLFTSESVSEGHPDKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQ
IARDTIKRIGYDNTDYGIDYKGCAVLVAYDKQSPDIAQGVDRAHDDNLDQGAGDQGLMFGYACDETPELMPLPIYLSHRL
VERQASLRRDGRLPWLRPDAKSQVTIRYVDGKPDSIDTVVLSTQHAPDIELPALREAVIEEIIKPTLPADLIKGDIKFLV
NPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGV
AEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLRPIYEKTAAYGHFGREEPEFSWEATDKALALAEAAGV
EPTARVA
>Mature_407_residues
MKPTVTTQEPEKVANDYLFTSESVSEGHPDKVADQISDAILDAILEQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQ
IARDTIKRIGYDNTDYGIDYKGCAVLVAYDKQSPDIAQGVDRAHDDNLDQGAGDQGLMFGYACDETPELMPLPIYLSHRL
VERQASLRRDGRLPWLRPDAKSQVTIRYVDGKPDSIDTVVLSTQHAPDIELPALREAVIEEIIKPTLPADLIKGDIKFLV
NPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGV
AEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLRPIYEKTAAYGHFGREEPEFSWEATDKALALAEAAGV
EPTARVA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=378, Percent_Identity=58.2010582010582, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI5174529, Length=378, Percent_Identity=56.3492063492063, Blast_Score=420, Evalue=1e-117,
Organism=Escherichia coli, GI1789311, Length=385, Percent_Identity=69.8701298701299, Blast_Score=539, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI25145633, Length=380, Percent_Identity=58.9473684210526, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=58.7765957446808, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538498, Length=376, Percent_Identity=59.0425531914894, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17551082, Length=389, Percent_Identity=56.0411311053985, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32565905, Length=376, Percent_Identity=53.4574468085106, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25145635, Length=332, Percent_Identity=57.5301204819277, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565911, Length=328, Percent_Identity=57.9268292682927, Blast_Score=359, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=58.1395348837209, Blast_Score=324, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=57.1428571428571, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6320710, Length=379, Percent_Identity=59.1029023746702, Blast_Score=433, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=58.7301587301587, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI45552159, Length=376, Percent_Identity=55.0531914893617, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580529, Length=376, Percent_Identity=55.0531914893617, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580537, Length=376, Percent_Identity=55.0531914893617, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580533, Length=376, Percent_Identity=55.0531914893617, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580535, Length=376, Percent_Identity=55.0531914893617, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580531, Length=376, Percent_Identity=55.0531914893617, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=54.5454545454545, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=54.5454545454545, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=52.2666666666667, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=54.6218487394958, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 44016; Mature: 44016

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTVTTQEPEKVANDYLFTSESVSEGHPDKVADQISDAILDAILEQDKYSRVAAETLCN
CCCCCCCCCHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TGLVVLAGEITTTANIDYIQIARDTIKRIGYDNTDYGIDYKGCAVLVAYDKQSPDIAQGV
CCEEEEEECEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHH
DRAHDDNLDQGAGDQGLMFGYACDETPELMPLPIYLSHRLVERQASLRRDGRLPWLRPDA
HHHCCCCCCCCCCCCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KSQVTIRYVDGKPDSIDTVVLSTQHAPDIELPALREAVIEEIIKPTLPADLIKGDIKFLV
CCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCEEEEE
NPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKN
CCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKML
HHHHHHHHHHHEEEHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
DLLRPIYEKTAAYGHFGREEPEFSWEATDKALALAEAAGVEPTARVA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKPTVTTQEPEKVANDYLFTSESVSEGHPDKVADQISDAILDAILEQDKYSRVAAETLCN
CCCCCCCCCHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TGLVVLAGEITTTANIDYIQIARDTIKRIGYDNTDYGIDYKGCAVLVAYDKQSPDIAQGV
CCEEEEEECEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHH
DRAHDDNLDQGAGDQGLMFGYACDETPELMPLPIYLSHRLVERQASLRRDGRLPWLRPDA
HHHCCCCCCCCCCCCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KSQVTIRYVDGKPDSIDTVVLSTQHAPDIELPALREAVIEEIIKPTLPADLIKGDIKFLV
CCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCEEEEE
NPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKN
CCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKML
HHHHHHHHHHHEEEHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
DLLRPIYEKTAAYGHFGREEPEFSWEATDKALALAEAAGVEPTARVA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA