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The map label for this gene is ttcA [H]

Identifier: 161526144

GI number: 161526144

Start: 3255800

End: 3256795

Strand: Direct

Name: ttcA [H]

Synonym: Bmul_2975

Alternate gene names: 161526144

Gene position: 3255800-3256795 (Clockwise)

Preceding gene: 161526143

Following gene: 161526145

Centisome position: 94.41

GC content: 65.16

Gene sequence:

>996_bases
ATGAACGCCCCCCAAACGAACGACACGGCGGCCGACGTCGCCACCATCGAAGCAAGCGCCGCCGAAGTCGGCCGCCGCGC
ACTGACGCGCCGCGAGCAGAAGGAAGCGTACGAGGACAACAAGCTGTTCAAGCGGATCGTGCGCCAGGTCGGTCAGGCGA
TCGGCGACTACAACATGATCGAGGACGGCGACAAGGTGATGGTGTGCCTGTCGGGCGGCAAGGACAGCTATGCGATGCTC
GACATCCTGCTGCGCCTGCGCGAGCGTGCGCCGATCGACTTCGACATCGTCGCGGTAAACCTCGACCAGAAGCAGCCGGG
CTTTCCCGAGCACGTGCTGCCCGAGTACCTGACGCAGATCGGCGTGCCGTTCCATATCGAGAACCAGGATACCTACAGCA
TCGTCAAGCGGCTCGTGCCGGAAGGCAAGACGACCTGCTCGCTGTGCTCGCGGCTGCGCCGCGGGATCCTGTACCGCGTC
GCCGGCGAGCTCGGCGCGACCAAGATCGCGCTCGGCCATCACCGCGACGACATCGTGCAGACGCTGCTGCTGAACATGTT
CTACGGCGGCAAGCTGAAGGGGATGCCGCCGAAGCTGCAGTCGGACGACGGCAAGAACGTCGTGATCCGGCCGCTCGCGT
ACGTGAAGGAAACCGATCTCGAGAAATACGCGGAGCTGCGCGAATTCCCGATCATTCCGTGCAATCTGTGCGGCAGCCAG
CCGAACCTGAAGCGCGCGGAAATGAAGGCGCTGATCCGCGAGTGGGACAAGCGCTTCCCGGGCCGTGTCGACAACATGTT
CAACGCGCTCGCGAACGTCGTGCCGTCGCATCTGATGGATACGACGCTGTTCCCGTTCGCGTCGCTGCGCGCGACCGGCC
AGGCCGACCCGCAGGGCGACATCGCGTTCGACGAGGAACCGTGCGCGAGCGGCGACGAGACGGCCGCGCCGGGCGGCGCG
AAACCGATCTCGATCGTCCAGTTCGACGACCTGTAA

Upstream 100 bases:

>100_bases
CCGTGCGCGCGGTACGCGTCTGTACCGAGAAACCGGACGCCTATCCGGACTGCGACGCCGTCGGCGTCGAAGTCTTTCGC
ATCAAGGACGAGGAGCGAGC

Downstream 100 bases:

>100_bases
GACAGGGCCGGAACGCCCGCCGGAGCAGGGTTCCGCCGCCCTGTTCCGGCCCCGCTCGAAGGTGGCGCAACGCCCGGAGC
GACGGGGGCCGCATGCTAAA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNAPQTNDTAADVATIEASAAEVGRRALTRREQKEAYEDNKLFKRIVRQVGQAIGDYNMIEDGDKVMVCLSGGKDSYAML
DILLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLTQIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRV
AGELGATKIALGHHRDDIVQTLLLNMFYGGKLKGMPPKLQSDDGKNVVIRPLAYVKETDLEKYAELREFPIIPCNLCGSQ
PNLKRAEMKALIREWDKRFPGRVDNMFNALANVVPSHLMDTTLFPFASLRATGQADPQGDIAFDEEPCASGDETAAPGGA
KPISIVQFDDL

Sequences:

>Translated_331_residues
MNAPQTNDTAADVATIEASAAEVGRRALTRREQKEAYEDNKLFKRIVRQVGQAIGDYNMIEDGDKVMVCLSGGKDSYAML
DILLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLTQIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRV
AGELGATKIALGHHRDDIVQTLLLNMFYGGKLKGMPPKLQSDDGKNVVIRPLAYVKETDLEKYAELREFPIIPCNLCGSQ
PNLKRAEMKALIREWDKRFPGRVDNMFNALANVVPSHLMDTTLFPFASLRATGQADPQGDIAFDEEPCASGDETAAPGGA
KPISIVQFDDL
>Mature_331_residues
MNAPQTNDTAADVATIEASAAEVGRRALTRREQKEAYEDNKLFKRIVRQVGQAIGDYNMIEDGDKVMVCLSGGKDSYAML
DILLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLTQIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRV
AGELGATKIALGHHRDDIVQTLLLNMFYGGKLKGMPPKLQSDDGKNVVIRPLAYVKETDLEKYAELREFPIIPCNLCGSQ
PNLKRAEMKALIREWDKRFPGRVDNMFNALANVVPSHLMDTTLFPFASLRATGQADPQGDIAFDEEPCASGDETAAPGGA
KPISIVQFDDL

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=274, Percent_Identity=67.1532846715328, Blast_Score=405, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI193211090, Length=271, Percent_Identity=30.6273062730627, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17540090, Length=222, Percent_Identity=27.4774774774775, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24651972, Length=248, Percent_Identity=27.8225806451613, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 36749; Mature: 36749

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPQTNDTAADVATIEASAAEVGRRALTRREQKEAYEDNKLFKRIVRQVGQAIGDYNMI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EDGDKVMVCLSGGKDSYAMLDILLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLTQI
CCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHC
GVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRVAGELGATKIALGHHRDDIVQ
CCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
TLLLNMFYGGKLKGMPPKLQSDDGKNVVIRPLAYVKETDLEKYAELREFPIIPCNLCGSQ
HHHHHHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCHHCCCCC
PNLKRAEMKALIREWDKRFPGRVDNMFNALANVVPSHLMDTTLFPFASLRATGQADPQGD
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
IAFDEEPCASGDETAAPGGAKPISIVQFDDL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MNAPQTNDTAADVATIEASAAEVGRRALTRREQKEAYEDNKLFKRIVRQVGQAIGDYNMI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EDGDKVMVCLSGGKDSYAMLDILLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLTQI
CCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHC
GVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRVAGELGATKIALGHHRDDIVQ
CCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
TLLLNMFYGGKLKGMPPKLQSDDGKNVVIRPLAYVKETDLEKYAELREFPIIPCNLCGSQ
HHHHHHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCHHCCCCC
PNLKRAEMKALIREWDKRFPGRVDNMFNALANVVPSHLMDTTLFPFASLRATGQADPQGD
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
IAFDEEPCASGDETAAPGGAKPISIVQFDDL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA