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The map label for this gene is yafC [C]

Identifier: 161526131

GI number: 161526131

Start: 3242052

End: 3243014

Strand: Reverse

Name: yafC [C]

Synonym: Bmul_2962

Alternate gene names: 161526131

Gene position: 3243014-3242052 (Counterclockwise)

Preceding gene: 161526132

Following gene: 161526129

Centisome position: 94.04

GC content: 67.5

Gene sequence:

>963_bases
ATGGATACCCTCGTCAGCATGAATGTGTTTCGCTACGTCGTCGAAGTGGGCAGCTTCGTCGGCGCGGCGGAGCGCATGCA
AATGTCGGCGGCGATGGCGAGCAAGCACGTGATGCACCTGGAGCAGCAGCTCGGCGCACGGCTGCTGCATCGTACGACGC
GACGCGTCGCGCCCACCGAGGCGGGACGCGAATATTACGAGCGCCTCGTGCAGGCGCTGTCCGAACTCGACGAAGCGGGA
CAGGCCGTCGGCGCCGCGAGCGTGGTGCCGCAAGGGCGGCTGCGCGTGACGTCGCTGTCCGCATTCGGCCTGCGTCACGT
GATGCAGGCCGTCACCGATTATGCGGAGCGCTTTCCGGACGTGACGGTCGAGATCACGCTGTCCGATCGTGTCGTCGAAC
TGATCGACGAGGGCTACGACGTCGCCGTGCGCGCGGCGCCGAGCGGACTGAAGTCGTCGTCGCTCGTGGCGCGCCAGATC
GCGACCGCGCACATTCTGCTCGTCGCGTCGCCCGAATATCTGAAAAAGCACGGGACGCCCGAGACGCTCGCGGATCTCGC
GCAGCACAACTATCTGCGGCGCGACTCGAACGCGTCGACGCTCGACGCGATGCTGAACGACGCGGCCGCCGCGTCGCGCG
TGGCGCTGAACGGCAATCTGATCGTCAACCATCTCGAAGGCCTGCGTGCGGCCGTGCTGGCCGGCGCCGGCATTGCGCTG
CTCGGCACCGAAGTGGTCGGCGACGACATCGAGGCGGGGCGCCTCGTGCCCGTGCTCGTCGATGCGGTGCCGCCGCGCGA
GCTGCCGATCTATGCGGTGTATGCGAGCCGTCGCCACGTGTCGGCGAAGGTGCGCTCGTTCGTCGATTTTCTCGCTGCGC
GCTTCGAAGGGCAGTCGCTGTGTCCGTCGATCGAATCGCGCTCGCACGTGCTGCCGATGTCGCGGATGAAGCGCACGGGC
TGA

Upstream 100 bases:

>100_bases
ATATCGCGGCGCCGTTTTGCCATTCTTGACCTTTCGCTTCGCGTGCCTTTGTTACCCTATCTCGCAATAGCATCATCACC
CGGAATTGCGAGCTAGTTAG

Downstream 100 bases:

>100_bases
ACGCGCCGCTGCGCATCGACATAAAAAAATCGCGGACCGTGCAGCAGACGGTCCGCGATTTTTTTTGTCCGACCGATTCG
CGGGTCGGCAACGTCACATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTEAGREYYERLVQALSELDEAG
QAVGAASVVPQGRLRVTSLSAFGLRHVMQAVTDYAERFPDVTVEITLSDRVVELIDEGYDVAVRAAPSGLKSSSLVARQI
ATAHILLVASPEYLKKHGTPETLADLAQHNYLRRDSNASTLDAMLNDAAAASRVALNGNLIVNHLEGLRAAVLAGAGIAL
LGTEVVGDDIEAGRLVPVLVDAVPPRELPIYAVYASRRHVSAKVRSFVDFLAARFEGQSLCPSIESRSHVLPMSRMKRTG

Sequences:

>Translated_320_residues
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTEAGREYYERLVQALSELDEAG
QAVGAASVVPQGRLRVTSLSAFGLRHVMQAVTDYAERFPDVTVEITLSDRVVELIDEGYDVAVRAAPSGLKSSSLVARQI
ATAHILLVASPEYLKKHGTPETLADLAQHNYLRRDSNASTLDAMLNDAAAASRVALNGNLIVNHLEGLRAAVLAGAGIAL
LGTEVVGDDIEAGRLVPVLVDAVPPRELPIYAVYASRRHVSAKVRSFVDFLAARFEGQSLCPSIESRSHVLPMSRMKRTG
>Mature_320_residues
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTEAGREYYERLVQALSELDEAG
QAVGAASVVPQGRLRVTSLSAFGLRHVMQAVTDYAERFPDVTVEITLSDRVVELIDEGYDVAVRAAPSGLKSSSLVARQI
ATAHILLVASPEYLKKHGTPETLADLAQHNYLRRDSNASTLDAMLNDAAAASRVALNGNLIVNHLEGLRAAVLAGAGIAL
LGTEVVGDDIEAGRLVPVLVDAVPPRELPIYAVYASRRHVSAKVRSFVDFLAARFEGQSLCPSIESRSHVLPMSRMKRTG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=35.8620689655172, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=35.2542372881356, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=30.4054054054054, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1787128, Length=293, Percent_Identity=30.3754266211604, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=29.6551724137931, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=29.4915254237288, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787589, Length=302, Percent_Identity=27.4834437086093, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1789173, Length=298, Percent_Identity=27.5167785234899, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788481, Length=296, Percent_Identity=25, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788706, Length=217, Percent_Identity=26.7281105990783, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789204, Length=298, Percent_Identity=23.8255033557047, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI157672245, Length=144, Percent_Identity=28.4722222222222, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34621; Mature: 34621

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGREYYERLVQALSELDEAGQAVGAASVVPQGRLRVTSLSAFGLRHVMQAVTDYAERFPD
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC
VTVEITLSDRVVELIDEGYDVAVRAAPSGLKSSSLVARQIATAHILLVASPEYLKKHGTP
CEEEEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHEEEEEECHHHHHHCCCH
ETLADLAQHNYLRRDSNASTLDAMLNDAAAASRVALNGNLIVNHLEGLRAAVLAGAGIAL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCHHH
LGTEVVGDDIEAGRLVPVLVDAVPPRELPIYAVYASRRHVSAKVRSFVDFLAARFEGQSL
HHHHHHCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
CPSIESRSHVLPMSRMKRTG
CCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGREYYERLVQALSELDEAGQAVGAASVVPQGRLRVTSLSAFGLRHVMQAVTDYAERFPD
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC
VTVEITLSDRVVELIDEGYDVAVRAAPSGLKSSSLVARQIATAHILLVASPEYLKKHGTP
CEEEEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHEEEEEECHHHHHHCCCH
ETLADLAQHNYLRRDSNASTLDAMLNDAAAASRVALNGNLIVNHLEGLRAAVLAGAGIAL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCHHH
LGTEVVGDDIEAGRLVPVLVDAVPPRELPIYAVYASRRHVSAKVRSFVDFLAARFEGQSL
HHHHHHCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
CPSIESRSHVLPMSRMKRTG
CCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]