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The map label for this gene is gcvA [H]

Identifier: 161526048

GI number: 161526048

Start: 3144332

End: 3145249

Strand: Direct

Name: gcvA [H]

Synonym: Bmul_2879

Alternate gene names: 161526048

Gene position: 3144332-3145249 (Clockwise)

Preceding gene: 161526044

Following gene: 161526049

Centisome position: 91.18

GC content: 71.13

Gene sequence:

>918_bases
ATGAACCGTTCCCGCCTGCCGCCGCTGAACGCGTTGCGCGCATTCGAGGCGGCCGCCCGCCATCTGTCGGTGAAGTCCGC
GGCCGAAGAACTGTCGGTCACGCCGGGCGCCGTGAGTCAGATGATCCGCACGCTCGAGACGCATCTCGGCGTGCAGCTGT
TCGAACGCGTGAACCGCGGCATCCTGCTGACCGCCGCGGGTCGCGACTACCTGCCGCCCGTGCGCAACGCGTTCCGGCAG
ATCGCCGACGCGTCGCAGCGCGTGTCGGGCGCGGCCGACAGCGGCGTGCTGACGATCAGCGTCACGCCGTTTTTTGCGTC
CGCGTGGCTCGTGCCGCGCCTCGCGCGGTTTCGCGACGCGTGTCCGGACGTCGATCTGCAGATCGTCACGAGTCATGCAC
TCGCGGATTTCGCACGCGACGGCGTGGACGTCGCGATTCGTCACGGCCTCGGCCGCTACGCCGGACTGTGCAGCGAGCAC
CTGCTGACGGTGGAGATCGTCGCGCTCGCGTCGCCCGAGCTCGTCGCGCGGCTCGGCATGCCGCGCACGCCGGCCGACCT
CGTCGACTGGCCGCATCTGCACGATGCCGAGCGCAAAGGCTGGCACACCTGGTTCGATGCGCAGCGCATCGACGATTTCG
GCCCGCCGCGCGGCCCCGCCTTCGACGATGCGGGCCTGCTGCTGCAGGCGATCGTCGCCGGGCAAGGCGCGGGGCTGCTG
CCGGCCGCGATGGTGCGCGGCGAGCTCGCGAACGGCCGGCTCGTGCAGCTCGCCGACGTCGCACGGCTCGACGATTTCGC
GTACTACCTCGTCTACCCGCCGCATCATGCGGAACGGCCGAAGGTGGCTGCGTTCCGGCGCTGGGTACTCGATGCCGCGC
GCGCCGGCGACGCGCCATCGATGTCCGGCGCGACGTAG

Upstream 100 bases:

>100_bases
CACGGCAACGTCGGCAACGCGATCCGACACACCCACGTTACTCGCGCGTGCGCAGCGCCTCAAACGATATTTGCTCGGCC
TTTCGATTGAGAAAAACTCA

Downstream 100 bases:

>100_bases
CGACAACGCGCGCCGCCGCCGGCCCGCGCCGCTTCTCCCGCGCATCGGTTAGCATGCCGCCCACGGCCATCGCGCCGCGC
CGATAACAACATCCCGAGAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNRSRLPPLNALRAFEAAARHLSVKSAAEELSVTPGAVSQMIRTLETHLGVQLFERVNRGILLTAAGRDYLPPVRNAFRQ
IADASQRVSGAADSGVLTISVTPFFASAWLVPRLARFRDACPDVDLQIVTSHALADFARDGVDVAIRHGLGRYAGLCSEH
LLTVEIVALASPELVARLGMPRTPADLVDWPHLHDAERKGWHTWFDAQRIDDFGPPRGPAFDDAGLLLQAIVAGQGAGLL
PAAMVRGELANGRLVQLADVARLDDFAYYLVYPPHHAERPKVAAFRRWVLDAARAGDAPSMSGAT

Sequences:

>Translated_305_residues
MNRSRLPPLNALRAFEAAARHLSVKSAAEELSVTPGAVSQMIRTLETHLGVQLFERVNRGILLTAAGRDYLPPVRNAFRQ
IADASQRVSGAADSGVLTISVTPFFASAWLVPRLARFRDACPDVDLQIVTSHALADFARDGVDVAIRHGLGRYAGLCSEH
LLTVEIVALASPELVARLGMPRTPADLVDWPHLHDAERKGWHTWFDAQRIDDFGPPRGPAFDDAGLLLQAIVAGQGAGLL
PAAMVRGELANGRLVQLADVARLDDFAYYLVYPPHHAERPKVAAFRRWVLDAARAGDAPSMSGAT
>Mature_305_residues
MNRSRLPPLNALRAFEAAARHLSVKSAAEELSVTPGAVSQMIRTLETHLGVQLFERVNRGILLTAAGRDYLPPVRNAFRQ
IADASQRVSGAADSGVLTISVTPFFASAWLVPRLARFRDACPDVDLQIVTSHALADFARDGVDVAIRHGLGRYAGLCSEH
LLTVEIVALASPELVARLGMPRTPADLVDWPHLHDAERKGWHTWFDAQRIDDFGPPRGPAFDDAGLLLQAIVAGQGAGLL
PAAMVRGELANGRLVQLADVARLDDFAYYLVYPPHHAERPKVAAFRRWVLDAARAGDAPSMSGAT

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=41.4383561643836, Blast_Score=217, Evalue=9e-58,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=30.1694915254237, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=27.7397260273973, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786401, Length=278, Percent_Identity=28.7769784172662, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1787128, Length=293, Percent_Identity=26.6211604095563, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1789639, Length=250, Percent_Identity=27.2, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081978, Length=265, Percent_Identity=32.0754716981132, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787879, Length=301, Percent_Identity=29.5681063122924, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787589, Length=291, Percent_Identity=23.3676975945017, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI157672245, Length=137, Percent_Identity=37.2262773722628, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693105, Length=190, Percent_Identity=25.2631578947368, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33024; Mature: 33024

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSRLPPLNALRAFEAAARHLSVKSAAEELSVTPGAVSQMIRTLETHLGVQLFERVNRG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ILLTAAGRDYLPPVRNAFRQIADASQRVSGAADSGVLTISVTPFFASAWLVPRLARFRDA
EEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHC
CPDVDLQIVTSHALADFARDGVDVAIRHGLGRYAGLCSEHLLTVEIVALASPELVARLGM
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHCCC
PRTPADLVDWPHLHDAERKGWHTWFDAQRIDDFGPPRGPAFDDAGLLLQAIVAGQGAGLL
CCCCHHHCCCCCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCH
PAAMVRGELANGRLVQLADVARLDDFAYYLVYPPHHAERPKVAAFRRWVLDAARAGDAPS
HHHHHHHHCCCCCEEEHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCC
MSGAT
CCCCC
>Mature Secondary Structure
MNRSRLPPLNALRAFEAAARHLSVKSAAEELSVTPGAVSQMIRTLETHLGVQLFERVNRG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ILLTAAGRDYLPPVRNAFRQIADASQRVSGAADSGVLTISVTPFFASAWLVPRLARFRDA
EEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHC
CPDVDLQIVTSHALADFARDGVDVAIRHGLGRYAGLCSEHLLTVEIVALASPELVARLGM
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHCCC
PRTPADLVDWPHLHDAERKGWHTWFDAQRIDDFGPPRGPAFDDAGLLLQAIVAGQGAGLL
CCCCHHHCCCCCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCH
PAAMVRGELANGRLVQLADVARLDDFAYYLVYPPHHAERPKVAAFRRWVLDAARAGDAPS
HHHHHHHHCCCCCEEEHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCC
MSGAT
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]