Click here to switch to the map view.

The map label for this gene is ispB [H]

Identifier: 161525972

GI number: 161525972

Start: 3056050

End: 3057045

Strand: Direct

Name: ispB [H]

Synonym: Bmul_2803

Alternate gene names: 161525972

Gene position: 3056050-3057045 (Clockwise)

Preceding gene: 161525967

Following gene: 161525973

Centisome position: 88.62

GC content: 66.16

Gene sequence:

>996_bases
ATGTCGTCGTCCACCGCCTCCCCCTCCCTCAGCGCCGCCCACCTGCTCGCCCCGATCGCCAGCGACATGGAGCAGGTGAA
TCGCGTTATCCGGCAAAGCCTCGCGTCCGACGTGCTGCTGATCAACCAGATCGCCGAGTACATTATCGGCGCGGGCGGCA
AGCGGCTGCGCCCGGCACTGCTGCTGCTCGTGGCCGGCGCGCTCGGCGAAACGTCGCATCAGCGGCACGTGCTGGCCGCC
GTCGTCGAGTTCATCCACACGGCGACGCTGCTGCACGACGATGTCGTCGACGAATCGGAGCTGCGACGCGGCCGCGAGAC
GGCCAACGCGCTGTTCGGCAATGCGGCGAGCGTGCTGGTGGGCGACTACCTCTATTCGCGCTCGTTCGAGATGATGGTCG
GCGTCGGCAAGATGCGCGTGATGGAGATCCTGTCCGAAGCGACGACGATCATTTCCGAAGGCGAAGTGCTGCAGCTTCTG
AACATGCACGACGCGGACGTCGACGAAACGCGCTACATGCAGGTGATTCGCTACAAGACGGCCAAGCTCTTCGAAGCATC
GGCGCGTCTGGGCGCGGTGCTCGCTGGCGCCGACGCACCGACCGAGGCCGCGGCCGCCGAATACGGCCGCCGGATCGGCA
CCGCGTTCCAGATCATGGACGACTGGCTCGATTACGCGGGCACGGCCGAAGCGATGGGCAAGAACGCCGGCGACGATCTG
CGCGAAGGCAAGCCGACGCTGCCGCTGATCTATCTGATCGAGCGCGGTACGCCGGAGCAGGCGGCGCTCGCGCGCGAAGC
GATCGAACAGGGCGGTACCGATCGGTTCGACACCATTTTCGATGCCATCACGCGCTCGGGCGCGCTCGATCACACGCTCG
AATGCGCACGCCAGGAAGCGCTGGCGGCCGCAGCGGCGATCACCGCGTTTCCCGATTCGACATACAAGGAAAGCCTGCTC
GCGCTGTGCTCGTATTCGACGTCGCGGCAGTCGTAA

Upstream 100 bases:

>100_bases
GCCTCGGCCGAACGGCCAAAATCGCGGGCGCGCAACGTGCGACAGACCGGAATATTGCCGATTCGCCTTATAATCCGCGG
CATCACCCAATTCCATCATC

Downstream 100 bases:

>100_bases
GGGCGCCGCATCAGGCGCGGCGGCAGGACGCCATGACCGCGCCGATCACGTGCTTCGAAAATTTTCTTTCCCAAGGTATT
CCATTTTCGAAAAAGCGCTG

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSSSTASPSLSAAHLLAPIASDMEQVNRVIRQSLASDVLLINQIAEYIIGAGGKRLRPALLLLVAGALGETSHQRHVLAA
VVEFIHTATLLHDDVVDESELRRGRETANALFGNAASVLVGDYLYSRSFEMMVGVGKMRVMEILSEATTIISEGEVLQLL
NMHDADVDETRYMQVIRYKTAKLFEASARLGAVLAGADAPTEAAAAEYGRRIGTAFQIMDDWLDYAGTAEAMGKNAGDDL
REGKPTLPLIYLIERGTPEQAALAREAIEQGGTDRFDTIFDAITRSGALDHTLECARQEALAAAAAITAFPDSTYKESLL
ALCSYSTSRQS

Sequences:

>Translated_331_residues
MSSSTASPSLSAAHLLAPIASDMEQVNRVIRQSLASDVLLINQIAEYIIGAGGKRLRPALLLLVAGALGETSHQRHVLAA
VVEFIHTATLLHDDVVDESELRRGRETANALFGNAASVLVGDYLYSRSFEMMVGVGKMRVMEILSEATTIISEGEVLQLL
NMHDADVDETRYMQVIRYKTAKLFEASARLGAVLAGADAPTEAAAAEYGRRIGTAFQIMDDWLDYAGTAEAMGKNAGDDL
REGKPTLPLIYLIERGTPEQAALAREAIEQGGTDRFDTIFDAITRSGALDHTLECARQEALAAAAAITAFPDSTYKESLL
ALCSYSTSRQS
>Mature_330_residues
SSSTASPSLSAAHLLAPIASDMEQVNRVIRQSLASDVLLINQIAEYIIGAGGKRLRPALLLLVAGALGETSHQRHVLAAV
VEFIHTATLLHDDVVDESELRRGRETANALFGNAASVLVGDYLYSRSFEMMVGVGKMRVMEILSEATTIISEGEVLQLLN
MHDADVDETRYMQVIRYKTAKLFEASARLGAVLAGADAPTEAAAAEYGRRIGTAFQIMDDWLDYAGTAEAMGKNAGDDLR
EGKPTLPLIYLIERGTPEQAALAREAIEQGGTDRFDTIFDAITRSGALDHTLECARQEALAAAAAITAFPDSTYKESLLA
LCSYSTSRQS

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=308, Percent_Identity=29.2207792207792, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI4758430, Length=209, Percent_Identity=23.444976076555, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI83700220, Length=209, Percent_Identity=23.444976076555, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1789578, Length=311, Percent_Identity=51.4469453376206, Blast_Score=331, Evalue=4e-92,
Organism=Escherichia coli, GI1786623, Length=244, Percent_Identity=31.9672131147541, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17505681, Length=323, Percent_Identity=32.1981424148607, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319475, Length=242, Percent_Identity=30.5785123966942, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325188, Length=236, Percent_Identity=24.1525423728814, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24651612, Length=287, Percent_Identity=30.6620209059233, Blast_Score=142, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35611; Mature: 35480

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSTASPSLSAAHLLAPIASDMEQVNRVIRQSLASDVLLINQIAEYIIGAGGKRLRPAL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LLLVAGALGETSHQRHVLAAVVEFIHTATLLHDDVVDESELRRGRETANALFGNAASVLV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHH
GDYLYSRSFEMMVGVGKMRVMEILSEATTIISEGEVLQLLNMHDADVDETRYMQVIRYKT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHH
AKLFEASARLGAVLAGADAPTEAAAAEYGRRIGTAFQIMDDWLDYAGTAEAMGKNAGDDL
HHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHH
REGKPTLPLIYLIERGTPEQAALAREAIEQGGTDRFDTIFDAITRSGALDHTLECARQEA
HCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
LAAAAAITAFPDSTYKESLLALCSYSTSRQS
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSSTASPSLSAAHLLAPIASDMEQVNRVIRQSLASDVLLINQIAEYIIGAGGKRLRPAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LLLVAGALGETSHQRHVLAAVVEFIHTATLLHDDVVDESELRRGRETANALFGNAASVLV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHH
GDYLYSRSFEMMVGVGKMRVMEILSEATTIISEGEVLQLLNMHDADVDETRYMQVIRYKT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHH
AKLFEASARLGAVLAGADAPTEAAAAEYGRRIGTAFQIMDDWLDYAGTAEAMGKNAGDDL
HHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHH
REGKPTLPLIYLIERGTPEQAALAREAIEQGGTDRFDTIFDAITRSGALDHTLECARQEA
HCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
LAAAAAITAFPDSTYKESLLALCSYSTSRQS
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]