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The map label for this gene is tilS/hprT [H]

Identifier: 161525963

GI number: 161525963

Start: 3048124

End: 3048675

Strand: Direct

Name: tilS/hprT [H]

Synonym: Bmul_2794

Alternate gene names: 161525963

Gene position: 3048124-3048675 (Clockwise)

Preceding gene: 161525962

Following gene: 161525966

Centisome position: 88.39

GC content: 65.4

Gene sequence:

>552_bases
ATGAACCGCGAAGAAGCCCTCCACATTTTCGAACACTCCGAAGAGATCGTCTCGGCGGACGCCGTCAACGCATCGATCGT
CCGGATGGCCGACGCGATCCGCGCCGAGATCGGCGACGCGTTCCCGCTCGTTCTGTCGGTGATGGGCGGCGCGGCGGTGT
TTACCGGGATGCTGCTGCCGCATCTCGATTTCCCGCTCGAATTCGACTACATCCACCTGACGCGCTACCGCAACACGACG
CAGGGCAGCCCGGAGATGCACTGGCGCGTCGCACCGCGCGAGTCGGTGAAAGACCGCATCGTGCTCGTGCTCGACGACAT
CCTCGACGAAGGCGAGACGATGGCCGCGATCCGCGACCGCATTCTCGACATGGGCGCGAAGCGCTTCATGTCGGCCGTGC
TGTGCGAGAAGACGCTCGCGAAGGCGAAGCCGCTGCATCCCGACTTCTGCGGCTTCTCGGTGCCGGACCGCTACGTGTTC
GGCTGCGGGATGGACGCGAAGGGCTACTGGCGCAACCTGCCGACGATCCGCGCGCTGACCGCGAACGTCTGA

Upstream 100 bases:

>100_bases
CGCTGATTCCGTCCGCCGGCCGGCGCGCGGGTTTTTGCCGTAGCGCGCACCGGCCGTTGTGCTTCACTCTTATTTGTATA
CCGACTGTCCGCCAGAACAC

Downstream 100 bases:

>100_bases
GCGCACGCCGGCTTCGCCGTCGCCAAACGGAAAGCGGCGCCCGGGTTCATGCCCGGAGCGCCGCTTTTTTTCGTCGTCGC
GCGCGAAACGCGCGTCAGGT

Product: hypoxanthine-guanine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MNREEALHIFEHSEEIVSADAVNASIVRMADAIRAEIGDAFPLVLSVMGGAAVFTGMLLPHLDFPLEFDYIHLTRYRNTT
QGSPEMHWRVAPRESVKDRIVLVLDDILDEGETMAAIRDRILDMGAKRFMSAVLCEKTLAKAKPLHPDFCGFSVPDRYVF
GCGMDAKGYWRNLPTIRALTANV

Sequences:

>Translated_183_residues
MNREEALHIFEHSEEIVSADAVNASIVRMADAIRAEIGDAFPLVLSVMGGAAVFTGMLLPHLDFPLEFDYIHLTRYRNTT
QGSPEMHWRVAPRESVKDRIVLVLDDILDEGETMAAIRDRILDMGAKRFMSAVLCEKTLAKAKPLHPDFCGFSVPDRYVF
GCGMDAKGYWRNLPTIRALTANV
>Mature_183_residues
MNREEALHIFEHSEEIVSADAVNASIVRMADAIRAEIGDAFPLVLSVMGGAAVFTGMLLPHLDFPLEFDYIHLTRYRNTT
QGSPEMHWRVAPRESVKDRIVLVLDDILDEGETMAAIRDRILDMGAKRFMSAVLCEKTLAKAKPLHPDFCGFSVPDRYVF
GCGMDAKGYWRNLPTIRALTANV

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17510817, Length=165, Percent_Identity=26.0606060606061, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 20481; Mature: 20481

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREEALHIFEHSEEIVSADAVNASIVRMADAIRAEIGDAFPLVLSVMGGAAVFTGMLLP
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
HLDFPLEFDYIHLTRYRNTTQGSPEMHWRVAPRESVKDRIVLVLDDILDEGETMAAIRDR
CCCCCCCCCEEEEEEECCCCCCCCCEEEEECCHHHHHHHEEHHHHHHHCCCHHHHHHHHH
ILDMGAKRFMSAVLCEKTLAKAKPLHPDFCGFSVPDRYVFGCGMDAKGYWRNLPTIRALT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHCCCHHHHEE
ANV
CCC
>Mature Secondary Structure
MNREEALHIFEHSEEIVSADAVNASIVRMADAIRAEIGDAFPLVLSVMGGAAVFTGMLLP
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
HLDFPLEFDYIHLTRYRNTTQGSPEMHWRVAPRESVKDRIVLVLDDILDEGETMAAIRDR
CCCCCCCCCEEEEEEECCCCCCCCCEEEEECCHHHHHHHEEHHHHHHHCCCHHHHHHHHH
ILDMGAKRFMSAVLCEKTLAKAKPLHPDFCGFSVPDRYVFGCGMDAKGYWRNLPTIRALT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHCCCHHHHEE
ANV
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]