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The map label for this gene is yiaJ [H]

Identifier: 161525937

GI number: 161525937

Start: 3020421

End: 3021320

Strand: Reverse

Name: yiaJ [H]

Synonym: Bmul_2768

Alternate gene names: 161525937

Gene position: 3021320-3020421 (Counterclockwise)

Preceding gene: 161525938

Following gene: 161525936

Centisome position: 87.61

GC content: 70.56

Gene sequence:

>900_bases
ATGACCAAGATGCCCGACTCCCTCGCCCTCGACGCCCGGCGCGCGACGCCGGACGATGCGCCGCTCAGCGACAGCATCGG
CGCGACCAGCACGCCGCTCGATCTTGCCGCGCAGCAGGCCGGCACGCAGACGCTGTTGCGCGGCCTCGCGATTCTCGAAG
CGATCGCGAACGGCGCGCGCGACATGCGCGCGATCGGCGCTGCGCTCGGCACGACGCGCAGCACCACGCATCGTCTCGTC
AGCAGCCTCGTGCAGGCGCGCTATCTGCGCCAGGTGCAGGGCGGCTATCTGCTCGGCCCGAAGCTGATCGAACTCGGCAC
GATCGCGCTCGAGCAGATGCCGCTGACCGCCGTTGCGCGGCCGCATCTCGAGGCGCTTGCCGAAGCGACGCTCGACACGA
TCCATCTCGGCGTGCGCGACGGCGACGACGTGCTGTACATCGACAAGATTCCCGGCACGCGCGGGCTCGAAATGCGCTCG
CGCGTCGGCCACCGGATGCCGCTCGCCTCGACCGGCATCGGCAAGGCGATGATGCTCGACCTCGCGCCCGAGACGTGGCG
CACGCTGTTCGACGCCGCGCGGCGTGCGCTCGCCGGCGTCAGCTTCAAGCCCGACAACCGGCCCGACTCGGCGGCGTTCC
TGCAGCGGATGGCGCACTACGCGGCCGGCGGCTACACGTTCGACCTCGAGGAGAACGAGGCGTCGATCCGCTGCGTGGCC
GCGCCGATCCGCGATGCGTCGGGCGCGATCGTCGCGGCGGTGTCGGTCGCGAGCACGATTCCGTACATGCCGCACGACCG
GATGGACGAACTGATTCCGCTCGTGCAGCGCGAAGCGCGAGCCATTTCCGCGGAGCTGGGCTGGAGCCCGCCGCAGGGTA
CCCGCAGGATCAAACGATGA

Upstream 100 bases:

>100_bases
GCGCTTTCCTCGGTGCTTCGCGCGCCGCGTTGCCGCGGCCGGGCTCGTGCCCGGCGCGCAGGACGCGGTTTCACCGATCC
CGTTGAAGTAGCCCCGTCCC

Downstream 100 bases:

>100_bases
CGAATCCGACCCGGACCGTTCCGGATGCCGCCGGCGCCGCAGCGTCGCTGATCGCGCTCGACTGGGGCACGACGTCGCTG
CGCGCCTATCTGTACGATGC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTKMPDSLALDARRATPDDAPLSDSIGATSTPLDLAAQQAGTQTLLRGLAILEAIANGARDMRAIGAALGTTRSTTHRLV
SSLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVARPHLEALAEATLDTIHLGVRDGDDVLYIDKIPGTRGLEMRS
RVGHRMPLASTGIGKAMMLDLAPETWRTLFDAARRALAGVSFKPDNRPDSAAFLQRMAHYAAGGYTFDLEENEASIRCVA
APIRDASGAIVAAVSVASTIPYMPHDRMDELIPLVQREARAISAELGWSPPQGTRRIKR

Sequences:

>Translated_299_residues
MTKMPDSLALDARRATPDDAPLSDSIGATSTPLDLAAQQAGTQTLLRGLAILEAIANGARDMRAIGAALGTTRSTTHRLV
SSLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVARPHLEALAEATLDTIHLGVRDGDDVLYIDKIPGTRGLEMRS
RVGHRMPLASTGIGKAMMLDLAPETWRTLFDAARRALAGVSFKPDNRPDSAAFLQRMAHYAAGGYTFDLEENEASIRCVA
APIRDASGAIVAAVSVASTIPYMPHDRMDELIPLVQREARAISAELGWSPPQGTRRIKR
>Mature_298_residues
TKMPDSLALDARRATPDDAPLSDSIGATSTPLDLAAQQAGTQTLLRGLAILEAIANGARDMRAIGAALGTTRSTTHRLVS
SLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVARPHLEALAEATLDTIHLGVRDGDDVLYIDKIPGTRGLEMRSR
VGHRMPLASTGIGKAMMLDLAPETWRTLFDAARRALAGVSFKPDNRPDSAAFLQRMAHYAAGGYTFDLEENEASIRCVAA
PIRDASGAIVAAVSVASTIPYMPHDRMDELIPLVQREARAISAELGWSPPQGTRRIKR

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1788131, Length=266, Percent_Identity=31.5789473684211, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1789999, Length=255, Percent_Identity=30.1960784313725, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1786468, Length=250, Percent_Identity=29.2, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082359, Length=252, Percent_Identity=29.7619047619048, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786715, Length=253, Percent_Identity=28.8537549407115, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788581, Length=251, Percent_Identity=30.2788844621514, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790752, Length=256, Percent_Identity=23.828125, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 32109; Mature: 31978

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKMPDSLALDARRATPDDAPLSDSIGATSTPLDLAAQQAGTQTLLRGLAILEAIANGAR
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHH
DMRAIGAALGTTRSTTHRLVSSLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVAR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCHHHHHH
PHLEALAEATLDTIHLGVRDGDDVLYIDKIPGTRGLEMRSRVGHRMPLASTGIGKAMMLD
HHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCHHHEEC
LAPETWRTLFDAARRALAGVSFKPDNRPDSAAFLQRMAHYAAGGYTFDLEENEASIRCVA
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEE
APIRDASGAIVAAVSVASTIPYMPHDRMDELIPLVQREARAISAELGWSPPQGTRRIKR
ECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC
>Mature Secondary Structure 
TKMPDSLALDARRATPDDAPLSDSIGATSTPLDLAAQQAGTQTLLRGLAILEAIANGAR
CCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHH
DMRAIGAALGTTRSTTHRLVSSLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVAR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCHHHHHH
PHLEALAEATLDTIHLGVRDGDDVLYIDKIPGTRGLEMRSRVGHRMPLASTGIGKAMMLD
HHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCHHHEEC
LAPETWRTLFDAARRALAGVSFKPDNRPDSAAFLQRMAHYAAGGYTFDLEENEASIRCVA
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEE
APIRDASGAIVAAVSVASTIPYMPHDRMDELIPLVQREARAISAELGWSPPQGTRRIKR
ECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]