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The map label for this gene is puuB [H]

Identifier: 161525887

GI number: 161525887

Start: 2970061

End: 2971479

Strand: Reverse

Name: puuB [H]

Synonym: Bmul_2718

Alternate gene names: 161525887

Gene position: 2971479-2970061 (Counterclockwise)

Preceding gene: 161525889

Following gene: 161525885

Centisome position: 86.17

GC content: 67.79

Gene sequence:

>1419_bases
ATGACTTCCATTCCCTCGCTGCTCGAGCGCGAATCGCGTTTCGAAGCCACGCAACTGAATATCGAAAAAGACAACTGGAA
CTGGTGCGACCCGGTACGCTACGACGGCGAACGGCCGGCGAACTGGTACGAGGCATCGCTGTCCCGCTGGGACAACGCGG
CGCCACTCGCGCACGATGCAGTATGCGACGTGCTGGTGATCGGCGCGGGTTTGCTCGGCGCGTCGGCCGCGCTGCATCTC
GCGGAGGCGGGCGTCGACACGATCCTCGTCGACAAGCATCATGTCGGCTCGGCCGCGTCCGGCCGCAACGGCGGTCAGCT
GACGCCCGGCCTCGCGCGCTGGGAAGCGGCCGACATGATCGAGCGTCTCGCGCATGAGGACGCGAAGCGGCTGTGGCGTT
TCGCGTCGTCGGAGTCGATGGCGCTGATCGATGCGATCGCCGCGCGCTACGCGCTCGACCTCGATCGCGGGCGCGGCCAC
GTGACGGCTGCCGTTCATCCCGGCCACATGAGCGCGCTGCTCGACGGCGCGGATGCGCGCCGTCATCTCGGCGACGCGAG
CGTGACGCTCGTCGGCAAGCATCAGCTGCACGACGAATACGTGCGCTCGCCGCTCTATCACGGCGCGGCGATCGATGCGC
TCGGCGGCCAGCTTCATCCGCTCGCGCTCGTGCGCGGCCTCGTGTACGGCTTCCGCGCCAACGACGGCGCGCTGTATGAA
TGCACCGAGGTGCTCGAACTCGACGAAACGCCCGCGGGCGTCGTCGCGACGACGCCGGGCGGCACGATCACCGCGCGCAA
GGGTGTCGTGCTCGCGCTGCACAACACGACGTTCCGGCTGTTCGACGACGGCGCGGCGACGACCGTGCCGTTCTTCACGT
ACGTGAGCGTGACGGCGCCGCTCGACGTCGACGTCGCATCGCTGATGCCGGCCGGCATGCCGGTGTACGACACGCAGTTC
CAGATCGACTACTACCGGCCGGTGCGCGGCAACCGGCTGCTGTTCGGCGGGCAGGGCACCGGTACCTGCTGGCCGCAGCA
CGATGCGAACGCGTATCTGCTCACGCGATTGAATGCGGTGTTTCCGCAATACGACGGCCGGTTCGCGCTCGACTACAGCT
GGAGCGGCGTCAGCGACTTCACGCTGAACGGCGCGACTGACAGCCGCAAGACCGACGGCCGCGTGCCGGTGTACATGGTG
CAGGGCTGGAGCGGGCACGGCGTCGCGCAGACGGTGCGGATCGGCAAGGCGATCTGCGACGACTTCGTCGGCCGCAACGA
CGACTTCTCGATGCTGACCGGCATCGACCATCGCGAGATTCCGCTCGGCCGTCAGCTGTCGCCGGTTGCGATTCCGGCCG
CGAAGGCGGCGATGAGCGTGATGACCGCGCTGAATCCGGGGAAGATGATCTCGTTCTGA

Upstream 100 bases:

>100_bases
GCGCCCCGTTTCTCGTTGACACCCAAGTGGAACCGGCTGCCCCACCCGCGAAGCCCGAACGGCTCTTCGACTGGCGGCCC
AAGACCATGCAAGTGCGAAC

Downstream 100 bases:

>100_bases
CGTATCGCCAATGCGCACCCGCACGCGACAACCGCGCATGAAAACGAAAACGCCCGATGCATCGCATCGGGCGTTTCGCT
ATGGAGCGTGCGGGCGACGA

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTSIPSLLERESRFEATQLNIEKDNWNWCDPVRYDGERPANWYEASLSRWDNAAPLAHDAVCDVLVIGAGLLGASAALHL
AEAGVDTILVDKHHVGSAASGRNGGQLTPGLARWEAADMIERLAHEDAKRLWRFASSESMALIDAIAARYALDLDRGRGH
VTAAVHPGHMSALLDGADARRHLGDASVTLVGKHQLHDEYVRSPLYHGAAIDALGGQLHPLALVRGLVYGFRANDGALYE
CTEVLELDETPAGVVATTPGGTITARKGVVLALHNTTFRLFDDGAATTVPFFTYVSVTAPLDVDVASLMPAGMPVYDTQF
QIDYYRPVRGNRLLFGGQGTGTCWPQHDANAYLLTRLNAVFPQYDGRFALDYSWSGVSDFTLNGATDSRKTDGRVPVYMV
QGWSGHGVAQTVRIGKAICDDFVGRNDDFSMLTGIDHREIPLGRQLSPVAIPAAKAAMSVMTALNPGKMISF

Sequences:

>Translated_472_residues
MTSIPSLLERESRFEATQLNIEKDNWNWCDPVRYDGERPANWYEASLSRWDNAAPLAHDAVCDVLVIGAGLLGASAALHL
AEAGVDTILVDKHHVGSAASGRNGGQLTPGLARWEAADMIERLAHEDAKRLWRFASSESMALIDAIAARYALDLDRGRGH
VTAAVHPGHMSALLDGADARRHLGDASVTLVGKHQLHDEYVRSPLYHGAAIDALGGQLHPLALVRGLVYGFRANDGALYE
CTEVLELDETPAGVVATTPGGTITARKGVVLALHNTTFRLFDDGAATTVPFFTYVSVTAPLDVDVASLMPAGMPVYDTQF
QIDYYRPVRGNRLLFGGQGTGTCWPQHDANAYLLTRLNAVFPQYDGRFALDYSWSGVSDFTLNGATDSRKTDGRVPVYMV
QGWSGHGVAQTVRIGKAICDDFVGRNDDFSMLTGIDHREIPLGRQLSPVAIPAAKAAMSVMTALNPGKMISF
>Mature_471_residues
TSIPSLLERESRFEATQLNIEKDNWNWCDPVRYDGERPANWYEASLSRWDNAAPLAHDAVCDVLVIGAGLLGASAALHLA
EAGVDTILVDKHHVGSAASGRNGGQLTPGLARWEAADMIERLAHEDAKRLWRFASSESMALIDAIAARYALDLDRGRGHV
TAAVHPGHMSALLDGADARRHLGDASVTLVGKHQLHDEYVRSPLYHGAAIDALGGQLHPLALVRGLVYGFRANDGALYEC
TEVLELDETPAGVVATTPGGTITARKGVVLALHNTTFRLFDDGAATTVPFFTYVSVTAPLDVDVASLMPAGMPVYDTQFQ
IDYYRPVRGNRLLFGGQGTGTCWPQHDANAYLLTRLNAVFPQYDGRFALDYSWSGVSDFTLNGATDSRKTDGRVPVYMVQ
GWSGHGVAQTVRIGKAICDDFVGRNDDFSMLTGIDHREIPLGRQLSPVAIPAAKAAMSVMTALNPGKMISF

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=411, Percent_Identity=31.8734793187348, Blast_Score=182, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 51011; Mature: 50880

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIPSLLERESRFEATQLNIEKDNWNWCDPVRYDGERPANWYEASLSRWDNAAPLAHDA
CCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
VCDVLVIGAGLLGASAALHLAEAGVDTILVDKHHVGSAASGRNGGQLTPGLARWEAADMI
HHHHHHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ERLAHEDAKRLWRFASSESMALIDAIAARYALDLDRGRGHVTAAVHPGHMSALLDGADAR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEECCCCCEEEEEECCCHHHHHHCCCHHH
RHLGDASVTLVGKHQLHDEYVRSPLYHGAAIDALGGQLHPLALVRGLVYGFRANDGALYE
HHCCCCEEEEEECHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHEEECCCCCHHH
CTEVLELDETPAGVVATTPGGTITARKGVVLALHNTTFRLFDDGAATTVPFFTYVSVTAP
HHHHHHCCCCCCCEEEECCCCEEEECCCEEEEEECCEEEEEECCCCCEEEEEEEEEEECC
LDVDVASLMPAGMPVYDTQFQIDYYRPVRGNRLLFGGQGTGTCWPQHDANAYLLTRLNAV
CCCCHHHHCCCCCCEECCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCEEEEEHHHHH
FPQYDGRFALDYSWSGVSDFTLNGATDSRKTDGRVPVYMVQGWSGHGVAQTVRIGKAICD
CCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
DFVGRNDDFSMLTGIDHREIPLGRQLSPVAIPAAKAAMSVMTALNPGKMISF
HHCCCCCCCHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TSIPSLLERESRFEATQLNIEKDNWNWCDPVRYDGERPANWYEASLSRWDNAAPLAHDA
CCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
VCDVLVIGAGLLGASAALHLAEAGVDTILVDKHHVGSAASGRNGGQLTPGLARWEAADMI
HHHHHHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ERLAHEDAKRLWRFASSESMALIDAIAARYALDLDRGRGHVTAAVHPGHMSALLDGADAR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEECCCCCEEEEEECCCHHHHHHCCCHHH
RHLGDASVTLVGKHQLHDEYVRSPLYHGAAIDALGGQLHPLALVRGLVYGFRANDGALYE
HHCCCCEEEEEECHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHEEECCCCCHHH
CTEVLELDETPAGVVATTPGGTITARKGVVLALHNTTFRLFDDGAATTVPFFTYVSVTAP
HHHHHHCCCCCCCEEEECCCCEEEECCCEEEEEECCEEEEEECCCCCEEEEEEEEEEECC
LDVDVASLMPAGMPVYDTQFQIDYYRPVRGNRLLFGGQGTGTCWPQHDANAYLLTRLNAV
CCCCHHHHCCCCCCEECCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCEEEEEHHHHH
FPQYDGRFALDYSWSGVSDFTLNGATDSRKTDGRVPVYMVQGWSGHGVAQTVRIGKAICD
CCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
DFVGRNDDFSMLTGIDHREIPLGRQLSPVAIPAAKAAMSVMTALNPGKMISF
HHCCCCCCCHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]