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The map label for this gene is gdhA [H]

Identifier: 161525884

GI number: 161525884

Start: 2965893

End: 2967179

Strand: Reverse

Name: gdhA [H]

Synonym: Bmul_2715

Alternate gene names: 161525884

Gene position: 2967179-2965893 (Counterclockwise)

Preceding gene: 161525885

Following gene: 161525883

Centisome position: 86.04

GC content: 65.97

Gene sequence:

>1287_bases
ATGTCTTCGCAACTGCAGTCCATCCCGTCCTACTTGCACGCCGACGATCTCGGCCCGTGGGGCAACTACCTTCAGCAGGT
CGATCGCGTCGCGCCGTACCTCGGTTCGCTGTCGCGCTGGCTAGAAACGCTGAAGCGTCCGAAGCGCATCCTGATCGTCG
ATGTGCCGATCGAGCTCGACAACGGTACCGTCGCGCACTTCGAAGGCTATCGCGTGCAGCACAACGTGTCGCGCGGCCCC
GGCAAGGGCGGCGTGCGCTACCACCAGGACGTGACGCTGTCGGAAGTGATGGCACTGTCGGCATGGATGTCGGTGAAGAA
CGCCGCGGTGAACGTGCCGTACGGCGGTGCGAAGGGCGGCATCCGCGTCGACCCGCGCAAGCTGTCGCGCGGCGAGCTCG
AGCGCGTCACGCGCCGCTACACGAGCGAAATCGGCATCATCATCGGCCCGAACACCGACATTCCGGCGCCGGACGTCAAC
ACCAACGAACAGGTGATGGCGTGGATGATGGACACGTTCTCGATGAACCAGGGCCAGACGTCGACCGGCGTCGTGACCGG
CAAGCCGATCTCGCTCGGCGGTTCGCTCGGCCGCAAGGAAGCGACCGGCCGCGGCGTGTTCGTGGTCGGCTGCGAAGCGG
CGAAGAAGAAGGGCCTCGAGATCGAAGGCGCGCGCATCGCGGTGCAGGGCTTCGGCAACGTCGGCGGGATCGCCGCGAAG
CTGTTCCAGGAAGCGGGCTCGAAGGTGATCGCGGTGCAGGATCACACGGGCACGATCTACCAGCCGGCCGGCCTCGACGC
GAACAAGCTGCTCGACCACGTCGCGCGCACGGGCGGCGTTGCGGGCTTCGAAGGCGCGGAGCCGATGGCGAACGACGAAT
TCTGGACGGTCGAGACCGACATCCTGATCCCGGCGGCGCTGGAGAACCAGATCACCGAGAAGAACGCGTCGAAGATCCGC
ACGAAGATCATCGTCGAAGGCGCGAACGGCCCGACGACGACCGCGGCCGACGACATCCTGAGCGCGAACGGCGTGCTCGT
GATCCCCGACGTCATCGCGAATGCGGGCGGCGTGACGGTGTCGTACTTCGAGTGGGTGCAGGATTTCTCGAGCTTCTTCT
GGACCGAAGACGAGATCAACCATCGCCTCGAGCGCGTGATGCGCGAAGCGTTCGCCGGCGTGTGGGCGGTCGCGGAAGAG
CACAAGGTGTCGGTGCGTACGGCGGCGTTCATCGTCGCGTGCAAGCGCATCCTGATGGCGCGCGAAATGCGCGGCCTGTA
CCCCTGA

Upstream 100 bases:

>100_bases
CGATCATGCAAAAAACGCATAATCGAATAAGCAAACGGCATTGGATAAAAAAAACGGGTTTTTCGACAATGTGCGCAATC
CGTTGAACGTTGGAGCATCC

Downstream 100 bases:

>100_bases
TCGGCGGCGCCGGCCGAACGCGGCCGGCGATCGCGATCGATCCATGACGCAATCCACCCGGTTGCGTGCATGAACCCGCG
CGGGCGGTGCCGAATCCGGC

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSSQLQSIPSYLHADDLGPWGNYLQQVDRVAPYLGSLSRWLETLKRPKRILIVDVPIELDNGTVAHFEGYRVQHNVSRGP
GKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRKLSRGELERVTRRYTSEIGIIIGPNTDIPAPDVN
TNEQVMAWMMDTFSMNQGQTSTGVVTGKPISLGGSLGRKEATGRGVFVVGCEAAKKKGLEIEGARIAVQGFGNVGGIAAK
LFQEAGSKVIAVQDHTGTIYQPAGLDANKLLDHVARTGGVAGFEGAEPMANDEFWTVETDILIPAALENQITEKNASKIR
TKIIVEGANGPTTTAADDILSANGVLVIPDVIANAGGVTVSYFEWVQDFSSFFWTEDEINHRLERVMREAFAGVWAVAEE
HKVSVRTAAFIVACKRILMAREMRGLYP

Sequences:

>Translated_428_residues
MSSQLQSIPSYLHADDLGPWGNYLQQVDRVAPYLGSLSRWLETLKRPKRILIVDVPIELDNGTVAHFEGYRVQHNVSRGP
GKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRKLSRGELERVTRRYTSEIGIIIGPNTDIPAPDVN
TNEQVMAWMMDTFSMNQGQTSTGVVTGKPISLGGSLGRKEATGRGVFVVGCEAAKKKGLEIEGARIAVQGFGNVGGIAAK
LFQEAGSKVIAVQDHTGTIYQPAGLDANKLLDHVARTGGVAGFEGAEPMANDEFWTVETDILIPAALENQITEKNASKIR
TKIIVEGANGPTTTAADDILSANGVLVIPDVIANAGGVTVSYFEWVQDFSSFFWTEDEINHRLERVMREAFAGVWAVAEE
HKVSVRTAAFIVACKRILMAREMRGLYP
>Mature_427_residues
SSQLQSIPSYLHADDLGPWGNYLQQVDRVAPYLGSLSRWLETLKRPKRILIVDVPIELDNGTVAHFEGYRVQHNVSRGPG
KGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRKLSRGELERVTRRYTSEIGIIIGPNTDIPAPDVNT
NEQVMAWMMDTFSMNQGQTSTGVVTGKPISLGGSLGRKEATGRGVFVVGCEAAKKKGLEIEGARIAVQGFGNVGGIAAKL
FQEAGSKVIAVQDHTGTIYQPAGLDANKLLDHVARTGGVAGFEGAEPMANDEFWTVETDILIPAALENQITEKNASKIRT
KIIVEGANGPTTTAADDILSANGVLVIPDVIANAGGVTVSYFEWVQDFSSFFWTEDEINHRLERVMREAFAGVWAVAEEH
KVSVRTAAFIVACKRILMAREMRGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=371, Percent_Identity=42.5876010781671, Blast_Score=284, Evalue=9e-77,
Organism=Homo sapiens, GI4885281, Length=371, Percent_Identity=41.7789757412399, Blast_Score=278, Evalue=7e-75,
Organism=Escherichia coli, GI1788059, Length=366, Percent_Identity=34.1530054644809, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17544676, Length=394, Percent_Identity=40.3553299492386, Blast_Score=270, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6319256, Length=400, Percent_Identity=29.5, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6324951, Length=377, Percent_Identity=29.7082228116711, Blast_Score=139, Evalue=7e-34,
Organism=Drosophila melanogaster, GI45553475, Length=362, Percent_Identity=41.9889502762431, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI45549226, Length=354, Percent_Identity=42.6553672316384, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24649283, Length=350, Percent_Identity=43.4285714285714, Blast_Score=271, Evalue=9e-73,
Organism=Drosophila melanogaster, GI24649460, Length=301, Percent_Identity=42.8571428571429, Blast_Score=234, Evalue=1e-61,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46364; Mature: 46233

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQLQSIPSYLHADDLGPWGNYLQQVDRVAPYLGSLSRWLETLKRPKRILIVDVPIELD
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEC
NGTVAHFEGYRVQHNVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGG
CCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCEEECCCCCCCCC
IRVDPRKLSRGELERVTRRYTSEIGIIIGPNTDIPAPDVNTNEQVMAWMMDTFSMNQGQT
CEECHHHCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
STGVVTGKPISLGGSLGRKEATGRGVFVVGCEAAKKKGLEIEGARIAVQGFGNVGGIAAK
CCCEEECCCEECCCCCCCCCCCCCEEEEEECHHHHHCCCEECCCEEEEEECCCCHHHHHH
LFQEAGSKVIAVQDHTGTIYQPAGLDANKLLDHVARTGGVAGFEGAEPMANDEFWTVETD
HHHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEC
ILIPAALENQITEKNASKIRTKIIVEGANGPTTTAADDILSANGVLVIPDVIANAGGVTV
EEECHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHCCCCEEEECHHHCCCCCCCH
SYFEWVQDFSSFFWTEDEINHRLERVMREAFAGVWAVAEEHKVSVRTAAFIVACKRILMA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
REMRGLYP
HHHHCCCC
>Mature Secondary Structure 
SSQLQSIPSYLHADDLGPWGNYLQQVDRVAPYLGSLSRWLETLKRPKRILIVDVPIELD
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEC
NGTVAHFEGYRVQHNVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGG
CCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCEEECCCCCCCCC
IRVDPRKLSRGELERVTRRYTSEIGIIIGPNTDIPAPDVNTNEQVMAWMMDTFSMNQGQT
CEECHHHCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
STGVVTGKPISLGGSLGRKEATGRGVFVVGCEAAKKKGLEIEGARIAVQGFGNVGGIAAK
CCCEEECCCEECCCCCCCCCCCCCEEEEEECHHHHHCCCEECCCEEEEEECCCCHHHHHH
LFQEAGSKVIAVQDHTGTIYQPAGLDANKLLDHVARTGGVAGFEGAEPMANDEFWTVETD
HHHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEC
ILIPAALENQITEKNASKIRTKIIVEGANGPTTTAADDILSANGVLVIPDVIANAGGVTV
EEECHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHCCCCEEEECHHHCCCCCCCH
SYFEWVQDFSSFFWTEDEINHRLERVMREAFAGVWAVAEEHKVSVRTAAFIVACKRILMA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
REMRGLYP
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]