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The map label for this gene is grpE [H]

Identifier: 161525804

GI number: 161525804

Start: 2871666

End: 2872211

Strand: Reverse

Name: grpE [H]

Synonym: Bmul_2635

Alternate gene names: 161525804

Gene position: 2872211-2871666 (Counterclockwise)

Preceding gene: 161525806

Following gene: 161525803

Centisome position: 83.29

GC content: 67.22

Gene sequence:

>546_bases
ATGGAAAACACGCAAGAGAACCCGGCATCCCAATCGGCCGAAGAAAACGGCAGCGAGACGCAGGCGGCACAAGACGCGGC
TCCCGCCGCGGAAGCAGCCGACGCGGCGCTCGCGGAGGCTCAAGCCAAGGTCGCCGAGCTGCAGGAAAGCTACCTGCGGG
CGAAGGCCGAGACCGAGAACGTTCGCCGTCGCGCGCAGGAAGACGTCGCGAAGGCCCATAAATTCGCGATCGAAAGCTTC
GCCGAGCACCTGCTGCCGGTGCTCGACAGCCTCGAAGCCGCCGTCGGCGACACGTCCGGCGACATCGCCAAGGTGCGCGA
AGGCGTCGAGCTCACGCTGCGCCAGCTGACGAGCGCGCTCGAGAAGGGCCGCGTCGTCGCGATCAATCCGGTCGGCGAGA
AGTTCGATCCGCACCGCCACCAGGCGATCTCGATGGTGCCGGCCGATCAGGAGCCGAACACTGTCGTCACGGTGCTGCAA
AAGGGCTACACGATCGCCGACCGCGTGCTGCGCCCGGCGCTCGTGACGGTCGCGCAGCCGAAGTAA

Upstream 100 bases:

>100_bases
GCGGTTTTTGCCCTTATCTGCACCAGCAATGTGCGGCACCTTCGGTCATACGTCCGGCGGTTGCCGATTTGGCTTCAACC
TCTAATCGACTTTCAGCGAC

Downstream 100 bases:

>100_bases
GGGCGTCGCCATGCTACCCGCCGGCGTCGATCCGGCCGCGTTCGACGCGTTCGGCATGCAGCCGCTGTCCGCCGACTCGT
TCGACGGCGGCATCGCCGGC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MENTQENPASQSAEENGSETQAAQDAAPAAEAADAALAEAQAKVAELQESYLRAKAETENVRRRAQEDVAKAHKFAIESF
AEHLLPVLDSLEAAVGDTSGDIAKVREGVELTLRQLTSALEKGRVVAINPVGEKFDPHRHQAISMVPADQEPNTVVTVLQ
KGYTIADRVLRPALVTVAQPK

Sequences:

>Translated_181_residues
MENTQENPASQSAEENGSETQAAQDAAPAAEAADAALAEAQAKVAELQESYLRAKAETENVRRRAQEDVAKAHKFAIESF
AEHLLPVLDSLEAAVGDTSGDIAKVREGVELTLRQLTSALEKGRVVAINPVGEKFDPHRHQAISMVPADQEPNTVVTVLQ
KGYTIADRVLRPALVTVAQPK
>Mature_181_residues
MENTQENPASQSAEENGSETQAAQDAAPAAEAADAALAEAQAKVAELQESYLRAKAETENVRRRAQEDVAKAHKFAIESF
AEHLLPVLDSLEAAVGDTSGDIAKVREGVELTLRQLTSALEKGRVVAINPVGEKFDPHRHQAISMVPADQEPNTVVTVLQ
KGYTIADRVLRPALVTVAQPK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=189, Percent_Identity=35.978835978836, Blast_Score=101, Evalue=4e-22,
Organism=Homo sapiens, GI40255109, Length=179, Percent_Identity=35.195530726257, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1788967, Length=191, Percent_Identity=40.8376963350785, Blast_Score=120, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17552458, Length=160, Percent_Identity=36.25, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=178, Percent_Identity=30.8988764044944, Blast_Score=92, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24653432, Length=168, Percent_Identity=35.1190476190476, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 19402; Mature: 19402

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTQENPASQSAEENGSETQAAQDAAPAAEAADAALAEAQAKVAELQESYLRAKAETEN
CCCCCCCCCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRRRAQEDVAKAHKFAIESFAEHLLPVLDSLEAAVGDTSGDIAKVREGVELTLRQLTSAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
EKGRVVAINPVGEKFDPHRHQAISMVPADQEPNTVVTVLQKGYTIADRVLRPALVTVAQP
HCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCC
K
C
>Mature Secondary Structure
MENTQENPASQSAEENGSETQAAQDAAPAAEAADAALAEAQAKVAELQESYLRAKAETEN
CCCCCCCCCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRRRAQEDVAKAHKFAIESFAEHLLPVLDSLEAAVGDTSGDIAKVREGVELTLRQLTSAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
EKGRVVAINPVGEKFDPHRHQAISMVPADQEPNTVVTVLQKGYTIADRVLRPALVTVAQP
HCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA