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The map label for this gene is ddh [H]

Identifier: 161525727

GI number: 161525727

Start: 2784693

End: 2785691

Strand: Direct

Name: ddh [H]

Synonym: Bmul_2558

Alternate gene names: 161525727

Gene position: 2784693-2785691 (Clockwise)

Preceding gene: 161525726

Following gene: 161525728

Centisome position: 80.75

GC content: 67.37

Gene sequence:

>999_bases
GTGCGCGTGATCCTGTTCAGCAGCCGACAGTACGACGACGATTCGTTCACCGCCGCCAACCGCCGGTTCGGCTACCGGCT
GCACTTCCAGCCGTCGCATCTCGATGCGGAAACGGCAATCCTCGCGCACGGCTACGACGTCGTGTGCCCGTTCGTCAACG
ACACGCTCGATGCGGCGGTGCTCGAACGCCTCGCGGAAGGCGGCACGCGGCTGATCGCGCTGCGCTCGGCGGGTTTCAAC
CATGTCGACCTCGCGGCGGCCGAGCGGCTCGGCATCACGGTCGTGCGCGTGCCGGCCTATTCGCCGCACGCGGTCGCCGA
ACATGCGGTCGCGCTGATTCTCGCGCTCAACCGCCGGCTGCCGCGCGCGGTCGCGCGCACGCGCGAAGGCGACTTCTCGC
TGAACGGGCTGCTCGGCTTCGACCTGCACGGCAAGACGGTCGGCGTGATCGGCACGGGGCTCATCGGCAGCGTGTTCGCG
AAGATCATGATGGGCTTCGGGATGCATGTGCTCGCGCATTCGATGCCGCCGTACGACGACGAACTGATCGCGTTCGGCGC
GCGCTACGTCGACCTCGACACGCTGCTGCGCGGCGCCGACATCGTGAGCCTGCACTGCCCGCTGCTGCCATCCACGCATC
ATCTGATCAATGCGCGCACGCTCGCGCAGATGAAGCCGGGCGCGATGCTGATCAACACGGGCCGCGGCGGGCTCGTCGAC
ACGCAGGCGCTGATCGATGCGCTCAAGAGCGGCCAGCTCGGTCATCTCGGCCTCGACGTCTACGAAGAGGAAAGCGGGCT
GTTCTTCGAGGATCACTCCGACCTGCCGCTGCAGGATGACGTGCTCGCGCGGCTGCTGACGTTCCCGAACGTGATCGTCA
CGTCGCATCAGGCGTTCTTCACGCGCGAAGCGCTGGCGGAAATCGCGCGTACGACGCTCGCGAACATCGAAGCGTGGCAT
GCGGGCACGCCGCAGAACGTCGTGACGCCGAGCCGCTGA

Upstream 100 bases:

>100_bases
GGGCTACGGTTTCCTGCGCGCGGCGCATGCGTCCGCGCATGCCGACGGCGTGCCGGGCCTGCAGTAAACTGCGGGCGACG
ACGCTCCCCGGAGAAACAGC

Downstream 100 bases:

>100_bases
TACGCCGGGCGCGCCTTCGGCCTTCCGCCTTCGGCCTCCCACCGCACACCGCACACCGCACGCTATCGCACACCTTTGCG
GCGCCGGCGTTTTTCGTCCA

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MRVILFSSRQYDDDSFTAANRRFGYRLHFQPSHLDAETAILAHGYDVVCPFVNDTLDAAVLERLAEGGTRLIALRSAGFN
HVDLAAAERLGITVVRVPAYSPHAVAEHAVALILALNRRLPRAVARTREGDFSLNGLLGFDLHGKTVGVIGTGLIGSVFA
KIMMGFGMHVLAHSMPPYDDELIAFGARYVDLDTLLRGADIVSLHCPLLPSTHHLINARTLAQMKPGAMLINTGRGGLVD
TQALIDALKSGQLGHLGLDVYEEESGLFFEDHSDLPLQDDVLARLLTFPNVIVTSHQAFFTREALAEIARTTLANIEAWH
AGTPQNVVTPSR

Sequences:

>Translated_332_residues
MRVILFSSRQYDDDSFTAANRRFGYRLHFQPSHLDAETAILAHGYDVVCPFVNDTLDAAVLERLAEGGTRLIALRSAGFN
HVDLAAAERLGITVVRVPAYSPHAVAEHAVALILALNRRLPRAVARTREGDFSLNGLLGFDLHGKTVGVIGTGLIGSVFA
KIMMGFGMHVLAHSMPPYDDELIAFGARYVDLDTLLRGADIVSLHCPLLPSTHHLINARTLAQMKPGAMLINTGRGGLVD
TQALIDALKSGQLGHLGLDVYEEESGLFFEDHSDLPLQDDVLARLLTFPNVIVTSHQAFFTREALAEIARTTLANIEAWH
AGTPQNVVTPSR
>Mature_332_residues
MRVILFSSRQYDDDSFTAANRRFGYRLHFQPSHLDAETAILAHGYDVVCPFVNDTLDAAVLERLAEGGTRLIALRSAGFN
HVDLAAAERLGITVVRVPAYSPHAVAEHAVALILALNRRLPRAVARTREGDFSLNGLLGFDLHGKTVGVIGTGLIGSVFA
KIMMGFGMHVLAHSMPPYDDELIAFGARYVDLDTLLRGADIVSLHCPLLPSTHHLINARTLAQMKPGAMLINTGRGGLVD
TQALIDALKSGQLGHLGLDVYEEESGLFFEDHSDLPLQDDVLARLLTFPNVIVTSHQAFFTREALAEIARTTLANIEAWH
AGTPQNVVTPSR

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=252, Percent_Identity=35.3174603174603, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI61743967, Length=249, Percent_Identity=37.7510040160643, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI4557497, Length=249, Percent_Identity=37.7510040160643, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI145580578, Length=257, Percent_Identity=35.0194552529183, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI4557499, Length=257, Percent_Identity=35.0194552529183, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI145580575, Length=257, Percent_Identity=35.0194552529183, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI6912396, Length=269, Percent_Identity=30.8550185873606, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1787645, Length=331, Percent_Identity=49.8489425981873, Blast_Score=327, Evalue=9e-91,
Organism=Escherichia coli, GI87082289, Length=308, Percent_Identity=32.4675324675325, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789279, Length=259, Percent_Identity=33.976833976834, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1788660, Length=213, Percent_Identity=31.4553990610329, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=257, Percent_Identity=30.3501945525292, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25147481, Length=263, Percent_Identity=27.7566539923954, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=203, Percent_Identity=35.4679802955665, Blast_Score=122, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6320925, Length=233, Percent_Identity=35.1931330472103, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=233, Percent_Identity=34.7639484978541, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324964, Length=220, Percent_Identity=28.6363636363636, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325144, Length=126, Percent_Identity=34.1269841269841, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321253, Length=129, Percent_Identity=34.8837209302326, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24646446, Length=264, Percent_Identity=37.5, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24646448, Length=264, Percent_Identity=37.5, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24646452, Length=264, Percent_Identity=37.5, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24646450, Length=264, Percent_Identity=37.5, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI62472511, Length=264, Percent_Identity=37.5, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19921140, Length=273, Percent_Identity=29.6703296703297, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI28574286, Length=276, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI28571528, Length=285, Percent_Identity=34.0350877192982, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI45552429, Length=270, Percent_Identity=32.2222222222222, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24585514, Length=270, Percent_Identity=32.2222222222222, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI28574282, Length=270, Percent_Identity=32.2222222222222, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI28574284, Length=270, Percent_Identity=32.2222222222222, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI45551003, Length=270, Percent_Identity=32.2222222222222, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24585516, Length=272, Percent_Identity=25.3676470588235, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 36100; Mature: 36100

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILFSSRQYDDDSFTAANRRFGYRLHFQPSHLDAETAILAHGYDVVCPFVNDTLDAAV
CEEEEECCCCCCCCCHHHHCCCCCEEEEECCCCCCCHHEEEECCCEEEECCCCCHHHHHH
LERLAEGGTRLIALRSAGFNHVDLAAAERLGITVVRVPAYSPHAVAEHAVALILALNRRL
HHHHHCCCCEEEEEECCCCCEEHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
PRAVARTREGDFSLNGLLGFDLHGKTVGVIGTGLIGSVFAKIMMGFGMHVLAHSMPPYDD
HHHHHHCCCCCEEECCEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHCCCCCCC
ELIAFGARYVDLDTLLRGADIVSLHCPLLPSTHHLINARTLAQMKPGAMLINTGRGGLVD
HHHHHCCCEEEHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCH
TQALIDALKSGQLGHLGLDVYEEESGLFFEDHSDLPLQDDVLARLLTFPNVIVTSHQAFF
HHHHHHHHHCCCCCEECCEEEECCCCCEEECCCCCCCCHHHHHHHHHCCCEEEECCHHHH
TREALAEIARTTLANIEAWHAGTPQNVVTPSR
HHHHHHHHHHHHHHCCCEECCCCCCCCCCCCC
>Mature Secondary Structure
MRVILFSSRQYDDDSFTAANRRFGYRLHFQPSHLDAETAILAHGYDVVCPFVNDTLDAAV
CEEEEECCCCCCCCCHHHHCCCCCEEEEECCCCCCCHHEEEECCCEEEECCCCCHHHHHH
LERLAEGGTRLIALRSAGFNHVDLAAAERLGITVVRVPAYSPHAVAEHAVALILALNRRL
HHHHHCCCCEEEEEECCCCCEEHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
PRAVARTREGDFSLNGLLGFDLHGKTVGVIGTGLIGSVFAKIMMGFGMHVLAHSMPPYDD
HHHHHHCCCCCEEECCEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHCCCCCCC
ELIAFGARYVDLDTLLRGADIVSLHCPLLPSTHHLINARTLAQMKPGAMLINTGRGGLVD
HHHHHCCCEEEHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCH
TQALIDALKSGQLGHLGLDVYEEESGLFFEDHSDLPLQDDVLARLLTFPNVIVTSHQAFF
HHHHHHHHHCCCCCEECCEEEECCCCCEEECCCCCCCCHHHHHHHHHCCCEEEECCHHHH
TREALAEIARTTLANIEAWHAGTPQNVVTPSR
HHHHHHHHHHHHHHCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]