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The map label for this gene is waaA [H]

Identifier: 161525663

GI number: 161525663

Start: 2708019

End: 2709359

Strand: Reverse

Name: waaA [H]

Synonym: Bmul_2494

Alternate gene names: 161525663

Gene position: 2709359-2708019 (Counterclockwise)

Preceding gene: 161525664

Following gene: 161525650

Centisome position: 78.57

GC content: 72.86

Gene sequence:

>1341_bases
ATGCTGAGGACGATCTATCGCGCGCTGTGGTGGCTCGTCGCGCCGCTCGCCGTCGTGCGGCTGTACGTGCGCTCGCGCAA
GGAGCACGGCTATCGCGAACATATCGGTGAGCGCTTCGGCTACGGCGCGGGCCGCTCGCCCGACGATCGCGCGCCGCTGA
TCTGGGTGCATGCGGTGTCGGTCGGCGAGACGCGCGCCGCGCAGCCGCTGATCGACGCGCTGATGCGCGCGCGGCCCGAC
GCGCGGATCCTGCTCACGCACATGACGCCGAGCGGCCGCGCGACGGGCGAGCAGCTGTTCGGCGATCGCGTGCTGCGCTG
CTATCTGCCGTACGACATGCCGGGCGCCGTGCGGCGCTTTCTGCGTGCATGGCGGCCGACGCTTGGCCTCGTGATGGAAA
CGGAAGTGTGGCCGACGCTGATCGACGAGTGCCGTCGCGCGGACGTGCCGCTCGTGCTGACGAATGCGCGGATGTCCGCG
CGTTCGTACCGGCGCGCGGCCAAGTTCGGCGCGGCGACGCGCGACGTGTTCGGCGGCTTCTCGCGCGTGCTCGCGCAGAG
CCCGGCGGATGCGGAGCGGCTGACGTCGCTCGGCGCGCGCAACGTGACGGTGCTCGGTAATCTGAAGTTCGACATGACGA
CGCCGCCCGAGCTGGCCGCGCGCGGCCATGCATGGCGCGACGCGATCGGCACGCGGCCGGTGTGGGTCGCGGCCAGCACG
CGCGAGAACGAGGAGGCGCTCGTGCTGCAGGCGTTCGCGGCGATGAAGACGCCCGACGCGCTGCTGCTGCTGGTGCCGCG
TCATCCGCAGCGTTTCGCGGAAGTCGAGGCGCTCGTCGCGCGTAACGGGCTCAAATGCGTGCGGCGTTCCGCGTGGGCGG
ACGACGCGGCCGCGCTCGCGGCGGGCCGGCCGGCTGCGGCCGAACCGCTGCCGGGCGACGTGACGGTGCTGCTCGGCGAT
TCGATGGGCGAGCTCGGCGCGTACTACGCGGCGTCGGACGTCGCGTTCATCGGTGGCAGCCTGTTGCCGCTCGGCGGGCA
GAACTTGATCGAGGCATGCGCGGTGGGCGTGCCGGTCGTGATCGGGCCGCACGTGTTCAACTTCACGCAGGCGACGGCCG
ACGCGGTCGCGGCCGGCGCGGCGCTGCAGGCCGCGGATCCGCTCGATCTCGCGCACGTGCTCGACGCGCTGTTCGCCGAC
AACGCGCGGCGCATTGCGATGGGCGCGGCCGCTGCGGCATTCGCGGCGCGCCATCGCGGCGCGACCGCGCGCACGGTCGA
CGTGCTGGCGGCGTTGCTGCCGCCGGTCGAGCGCGACACGTCCGTCACGCAGGACGACTGA

Upstream 100 bases:

>100_bases
GTGCGCCGCGAGTGCGCGCCGCTCGGCATCCTCGAGCGGCTCACGGGCAGGGCACTGGTTTGAGCGCGCCTATTCTTCGA
CACGACGGCCGGGCACACGC

Downstream 100 bases:

>100_bases
CGGCGAGCCGGCGCGGCGCGATCGCGCAGTGCCGGCGCCGCGCTGCCGCCGCCCATTTTCAGATCGCCGCCGGCCTCGCG
GCCATCCCGGTCGTGTGTGG

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: KDO2-lipid IVA; CMP; KDO1-lipid IVA [C]

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MLRTIYRALWWLVAPLAVVRLYVRSRKEHGYREHIGERFGYGAGRSPDDRAPLIWVHAVSVGETRAAQPLIDALMRARPD
ARILLTHMTPSGRATGEQLFGDRVLRCYLPYDMPGAVRRFLRAWRPTLGLVMETEVWPTLIDECRRADVPLVLTNARMSA
RSYRRAAKFGAATRDVFGGFSRVLAQSPADAERLTSLGARNVTVLGNLKFDMTTPPELAARGHAWRDAIGTRPVWVAAST
RENEEALVLQAFAAMKTPDALLLLVPRHPQRFAEVEALVARNGLKCVRRSAWADDAAALAAGRPAAAEPLPGDVTVLLGD
SMGELGAYYAASDVAFIGGSLLPLGGQNLIEACAVGVPVVIGPHVFNFTQATADAVAAGAALQAADPLDLAHVLDALFAD
NARRIAMGAAAAAFAARHRGATARTVDVLAALLPPVERDTSVTQDD

Sequences:

>Translated_446_residues
MLRTIYRALWWLVAPLAVVRLYVRSRKEHGYREHIGERFGYGAGRSPDDRAPLIWVHAVSVGETRAAQPLIDALMRARPD
ARILLTHMTPSGRATGEQLFGDRVLRCYLPYDMPGAVRRFLRAWRPTLGLVMETEVWPTLIDECRRADVPLVLTNARMSA
RSYRRAAKFGAATRDVFGGFSRVLAQSPADAERLTSLGARNVTVLGNLKFDMTTPPELAARGHAWRDAIGTRPVWVAAST
RENEEALVLQAFAAMKTPDALLLLVPRHPQRFAEVEALVARNGLKCVRRSAWADDAAALAAGRPAAAEPLPGDVTVLLGD
SMGELGAYYAASDVAFIGGSLLPLGGQNLIEACAVGVPVVIGPHVFNFTQATADAVAAGAALQAADPLDLAHVLDALFAD
NARRIAMGAAAAAFAARHRGATARTVDVLAALLPPVERDTSVTQDD
>Mature_446_residues
MLRTIYRALWWLVAPLAVVRLYVRSRKEHGYREHIGERFGYGAGRSPDDRAPLIWVHAVSVGETRAAQPLIDALMRARPD
ARILLTHMTPSGRATGEQLFGDRVLRCYLPYDMPGAVRRFLRAWRPTLGLVMETEVWPTLIDECRRADVPLVLTNARMSA
RSYRRAAKFGAATRDVFGGFSRVLAQSPADAERLTSLGARNVTVLGNLKFDMTTPPELAARGHAWRDAIGTRPVWVAAST
RENEEALVLQAFAAMKTPDALLLLVPRHPQRFAEVEALVARNGLKCVRRSAWADDAAALAAGRPAAAEPLPGDVTVLLGD
SMGELGAYYAASDVAFIGGSLLPLGGQNLIEACAVGVPVVIGPHVFNFTQATADAVAAGAALQAADPLDLAHVLDALFAD
NARRIAMGAAAAAFAARHRGATARTVDVLAALLPPVERDTSVTQDD

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=444, Percent_Identity=40.7657657657658, Blast_Score=301, Evalue=4e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 48042; Mature: 48042

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTIYRALWWLVAPLAVVRLYVRSRKEHGYREHIGERFGYGAGRSPDDRAPLIWVHAVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEE
VGETRAAQPLIDALMRARPDARILLTHMTPSGRATGEQLFGDRVLRCYLPYDMPGAVRRF
CCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCCEEEEECCCCCCHHHHHH
LRAWRPTLGLVMETEVWPTLIDECRRADVPLVLTNARMSARSYRRAAKFGAATRDVFGGF
HHHHCCHHCEEEECHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHH
SRVLAQSPADAERLTSLGARNVTVLGNLKFDMTTPPELAARGHAWRDAIGTRPVWVAAST
HHHHHCCCCCHHHHHHCCCCCEEEEEEEEECCCCCHHHHHCCCHHHHHCCCCCEEEEECC
RENEEALVLQAFAAMKTPDALLLLVPRHPQRFAEVEALVARNGLKCVRRSAWADDAAALA
CCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHH
AGRPAAAEPLPGDVTVLLGDSMGELGAYYAASDVAFIGGSLLPLGGQNLIEACAVGVPVV
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHCCCCEE
IGPHVFNFTQATADAVAAGAALQAADPLDLAHVLDALFADNARRIAMGAAAAAFAARHRG
ECCCHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
ATARTVDVLAALLPPVERDTSVTQDD
CCHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLRTIYRALWWLVAPLAVVRLYVRSRKEHGYREHIGERFGYGAGRSPDDRAPLIWVHAVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEE
VGETRAAQPLIDALMRARPDARILLTHMTPSGRATGEQLFGDRVLRCYLPYDMPGAVRRF
CCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCCEEEEECCCCCCHHHHHH
LRAWRPTLGLVMETEVWPTLIDECRRADVPLVLTNARMSARSYRRAAKFGAATRDVFGGF
HHHHCCHHCEEEECHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHH
SRVLAQSPADAERLTSLGARNVTVLGNLKFDMTTPPELAARGHAWRDAIGTRPVWVAAST
HHHHHCCCCCHHHHHHCCCCCEEEEEEEEECCCCCHHHHHCCCHHHHHCCCCCEEEEECC
RENEEALVLQAFAAMKTPDALLLLVPRHPQRFAEVEALVARNGLKCVRRSAWADDAAALA
CCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHH
AGRPAAAEPLPGDVTVLLGDSMGELGAYYAASDVAFIGGSLLPLGGQNLIEACAVGVPVV
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHCCCCEE
IGPHVFNFTQATADAVAAGAALQAADPLDLAHVLDALFADNARRIAMGAAAAAFAARHRG
ECCCHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
ATARTVDVLAALLPPVERDTSVTQDD
CCHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO1-lipid IVA; CMP-3-deoxy-D-manno-octulosonate; lipid IVA [C]

Specific reaction: KDO1-lipid IVA + CMP-3-deoxy-D-manno-octulosonate = KDO2-lipid IVA + CMP CMP-3-deoxy-D-manno-octulosonate + lipid IVA = KDO1-lipid IVA + CMP [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]