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The map label for this gene is citH [H]

Identifier: 161525540

GI number: 161525540

Start: 2581278

End: 2582711

Strand: Reverse

Name: citH [H]

Synonym: Bmul_2370

Alternate gene names: 161525540

Gene position: 2582711-2581278 (Counterclockwise)

Preceding gene: 161525541

Following gene: 161525538

Centisome position: 74.89

GC content: 66.39

Gene sequence:

>1434_bases
ATGCCGGCCGCCGCCGCGTTCATCGTGCGCAGCGGCCGGCACGGCGCGAGCGTACAGGTGTCGGGATCGGCACGGCGCAC
GCCGCGTCCGTTCATGCGGCGCCGACGGTCGCGCGCCCTTCGAGATCGAGACAAGGACATGTCCACAGCATCGCAAGCCA
TCACGCAGGAATCGAAAGTCCGCACCGTGTTTCGCGTCGTCAGCGGCAACTTCCTCGAAATGTACGACTTCATGGTCTAC
GGCTATTACGCGTCGGCGATCGCGAAGACCTATTTTCCGAGCGGCAACGCGTTCGCGTCATTGATGCTGTCGCTGTCGGT
GTTCGGCGCGGGCTTCCTGATGCGCCCGGTCGGCGCGATCGTGCTCGGCGCGTACATCGACCATCACGGACGCCGCAAGG
GGCTGATCCTGACGCTCACGCTGATGGCGCTCGGCACGCTGACCGTCGCCGCGGTGCCCGGCTATGCGACGATCGGTGTG
CTCGCACCGGTGCTCGTGCTGCTCGGGCGGCTGCTGCAGGGCTTTTCGGCCGGAGTCGAGCTGGGCGGCGTGTCGGTGTA
TCTGTCCGAGATCGCGACCAAGGGGCACAAGGGCTTCTACACGTCGTGGCAGTCGGGCAGCCAGCAGGTCGCGGTCGTGT
TCGCGGCGTTCGTCGGCGTGCTGCTGAACCGCACGCTGCCGGCCGAAGAGATGACGGCATGGGGCTGGCGCATCCCGTTC
CTGATCGGCTGCCTGATCGTGCCGTTCCTGTTCCTGATTCGCCGCTCGCTGAAGGAGACGGACGAGTTCCTCGCGAAGCG
CCATCGTCCGTCGATGGGCGAGATCATGCGCTCGATGCTCGACAACTGGGGGATTGTGATCGCGGGCATGGGCATGGTGA
TCATGACCACGGTGTCGTTCTACATGATCACCGCCTACACGCCCACGTTCGGCAAGGAAGTGCTGCACCTGTCGTCGCTC
GACGCGCTCGTCGTGACGGTCTGCATCGGAGTGTCGAACCTCGTCTGGCTGCCGCTGTCCGGTGCCCTGTCGGACCGCAT
CGGGCGCCGCCCCGTGCTGATCGCATTCACCGTGCTCACGCTGCTCACCGCGTACCCGACGGTATCGTGGCTCGTCGCCG
AGCCGTCGTTCCTGCGCTTGCTTGCCGTCGAGCTGTGGCTGTCGTTCCTTTACGGGTCGTACAACGGCGCGATGGTCGTC
GCGCTGACCGAAGTGATGCCGGCCGACGTGCGCACGGCCGGCTTCTCGCTCGCGTACAGCCTCGCGACGACGATCGGCGG
CTTCACGCCGGCGATCTCGACGCTGCTGATCCACGAAACCGCGAACAAGGCCGCGCCCGGGCTGTGGCTCAGCGTCGCCG
CGCTGTGCGGGCTGATCGCGACGCTCGTGCTGTACCGGACGCCGGAGTCGCGCAACCAATACAAGGCCGTCTGA

Upstream 100 bases:

>100_bases
GGCGCATGCTCTCCCGCAGCGGCACGACCCGCGCACGATCGCGACCGTGGACGGCCACCAAAAGGCAAATGTCGGGTTCG
CGTGGCATCATGGGCCGATC

Downstream 100 bases:

>100_bases
CGCGGCGCCGCGGCGCGGCCGGACGGTAACGAAGGACGGAGCGCGCGGCGCTCCGTTTTTTCATCCGCGCCCGGGCGGGG
CGAGCGCCTGCGCGACTGAG

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MPAAAAFIVRSGRHGASVQVSGSARRTPRPFMRRRRSRALRDRDKDMSTASQAITQESKVRTVFRVVSGNFLEMYDFMVY
GYYASAIAKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYIDHHGRRKGLILTLTLMALGTLTVAAVPGYATIGV
LAPVLVLLGRLLQGFSAGVELGGVSVYLSEIATKGHKGFYTSWQSGSQQVAVVFAAFVGVLLNRTLPAEEMTAWGWRIPF
LIGCLIVPFLFLIRRSLKETDEFLAKRHRPSMGEIMRSMLDNWGIVIAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSSL
DALVVTVCIGVSNLVWLPLSGALSDRIGRRPVLIAFTVLTLLTAYPTVSWLVAEPSFLRLLAVELWLSFLYGSYNGAMVV
ALTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHETANKAAPGLWLSVAALCGLIATLVLYRTPESRNQYKAV

Sequences:

>Translated_477_residues
MPAAAAFIVRSGRHGASVQVSGSARRTPRPFMRRRRSRALRDRDKDMSTASQAITQESKVRTVFRVVSGNFLEMYDFMVY
GYYASAIAKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYIDHHGRRKGLILTLTLMALGTLTVAAVPGYATIGV
LAPVLVLLGRLLQGFSAGVELGGVSVYLSEIATKGHKGFYTSWQSGSQQVAVVFAAFVGVLLNRTLPAEEMTAWGWRIPF
LIGCLIVPFLFLIRRSLKETDEFLAKRHRPSMGEIMRSMLDNWGIVIAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSSL
DALVVTVCIGVSNLVWLPLSGALSDRIGRRPVLIAFTVLTLLTAYPTVSWLVAEPSFLRLLAVELWLSFLYGSYNGAMVV
ALTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHETANKAAPGLWLSVAALCGLIATLVLYRTPESRNQYKAV
>Mature_476_residues
PAAAAFIVRSGRHGASVQVSGSARRTPRPFMRRRRSRALRDRDKDMSTASQAITQESKVRTVFRVVSGNFLEMYDFMVYG
YYASAIAKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYIDHHGRRKGLILTLTLMALGTLTVAAVPGYATIGVL
APVLVLLGRLLQGFSAGVELGGVSVYLSEIATKGHKGFYTSWQSGSQQVAVVFAAFVGVLLNRTLPAEEMTAWGWRIPFL
IGCLIVPFLFLIRRSLKETDEFLAKRHRPSMGEIMRSMLDNWGIVIAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSSLD
ALVVTVCIGVSNLVWLPLSGALSDRIGRRPVLIAFTVLTLLTAYPTVSWLVAEPSFLRLLAVELWLSFLYGSYNGAMVVA
LTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHETANKAAPGLWLSVAALCGLIATLVLYRTPESRNQYKAV

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=451, Percent_Identity=28.8248337028825, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI1788942, Length=428, Percent_Identity=33.6448598130841, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788292, Length=397, Percent_Identity=28.7153652392947, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789941, Length=424, Percent_Identity=29.2452830188679, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082231, Length=240, Percent_Identity=25.8333333333333, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 51782; Mature: 51651

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PAAAAFIVRSGRHGASVQVSGSARRTPRPFMRRRRSRALRDRDKDMSTASQAITQESKV
CCHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH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HHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]