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The map label for this gene is yhjG [H]

Identifier: 161525538

GI number: 161525538

Start: 2577590

End: 2579239

Strand: Reverse

Name: yhjG [H]

Synonym: Bmul_2368

Alternate gene names: 161525538

Gene position: 2579239-2577590 (Counterclockwise)

Preceding gene: 161525540

Following gene: 161525533

Centisome position: 74.79

GC content: 71.09

Gene sequence:

>1650_bases
ATGGCTGACACCCTGCTCGACATCCCGCCAGTGCTGATCGTCGGCGCCGGGCCTACGGGCCTGGCTGCGGCGATGAGCCT
CGCGCGTGCCCGCGTCCCGGTGCGCGTGATCGACAAGCTCGCGGCGCCGGCGCCGTATTCGCGCGCCATCGGCATCCAGG
CGCGCACGCTCGAACTGCTGGAGCAGCACCGCGCCGTCGAACCGTTTCTCGCGCTCGGGCATCGCGCGCACGCGGCGGCG
CTGCATGCCGACGGCCGCGTGATCGCGCGGCTCGATTTCGATCCATTGCAAACGCGCTATCCCTATCTGCTGCTGCTCGA
CCAGAGCGTCACCGAGCGGCTGCTGGCCGAGCATCTCGCCGAGCTCGGCGTGACGATCGAGCGCGGCGTGACGCTCGTCG
AATGCGATACGGGCGGCGCGTTGCTCGACGTGACGCTGCGCGATGCCGGCGGCCGCGACGAGCGGTTCTCGCCGTCGTAT
CTGATCGCGGCCGACGGCGCGCACAGCACGGTGCGGCACCTGCTCGGCATCGGCTTCGCGGGCCGCGCGTTCGAACAGAC
GTTTCTGCTCGCCGATTTCGCGGCGATTCCCGACTGGCCGGAAGACGAGATCCATCTGTTCACGACGCCCGACGGCATCG
CCGGGCTGTTTCCGCTCGGCAGCGGCCGCTATCGGCTCGTCGCCGACCGTCCGCCCGTCGGCGACGTGTCGCCCGACTCG
CCGCCGCCGTCGCTGGCCGACTGCGAAGCGGTCGTGCGGGCGCGCATTGGCGCCTCGATCGCGCCGAGCGATCTCGCGTG
GTCGTCGTATTTTCGGCTGCACAGCCGCATGGTCGAGCGGCTGCGGCACGGCCGCGTGTTCTTTGCGGGCGACGCCGCGC
ATATCCACAGCCCGGCCGGCGCGCAGGGGATGAACACGGGCGTGCAGGAGGCGTTCAACCTCGGCTGGAAGCTTGCGCGC
GTACTCGCGGGCGGGGCGCCCGACCGGCTGCTCGACACCTATCACTCGGAGCGTCATCCGATCGAGCGCGACGTGCTGCG
GCAGACGAGTCTGGTCACGCAGATCGTCGAAGCCGATCATGGCGCGATGAAGCTGCTGCGCGATCACCTGATGCCGCTGC
TGGTGTCGCTCGGGCCGCTGCGCGACGCGGTGCGGCGCAGTGTCAGCGAACTCGGCGTCCAGTACCGGAAGAGTCCGCTC
ACGCTCGAACGTGTGCTCGATGGCGGCCCGCGCGCGGGCGAACGCGCGCCCGATGCGTTGCTGCACGTGATCGACGGGCC
GCTCGGTCAGGCGCCCGGCACCGCGCGGCTATACGACGTGCACGACCCGGCGAGCTTCACGCTGCTGCTGCTCGAGGCGC
CGCCGAGCACCGGCCTCGACGACGTGCCGCCGCTGGCGGCCGACGCGCAAGCGCTGATCCAGCGCCTCGAACGGATCATG
CCGGGCGCGGTGCGTGCCTGGCGCGTGACCGATGTCGAAGGCGACGGTGCGGACACGCTGGTCCAGGCCTACGGCCGCTC
GCGTCCGGCGTTCTACCTCTTGCGGCCCGACGGCTACATCGCGGCACGCGGCCGCGCATCGAGCGACGCGAACGCGCTGC
TGCGACATTGCGAAACCTGGTTCGCGGGCGTTCCTTCGCCGTCTGCCTAG

Upstream 100 bases:

>100_bases
TCCCACAGCGCGCAGGCACGCCGCCGCTCGGCCGTTCGGCATATGGCCCCGTCACGCGGAGCGGCGTAGACTGGCAGCGT
CGTGTCCGATCGAGGAACTC

Downstream 100 bases:

>100_bases
CGCGCTAGGCCGTTGCGGCGGCCGGCACGAGCGAAGCGCGGTGCGTGGCTACCGCCTCGATCACGATCGGCGAGGCGCGC
CGCGCCGCTTCGCTTGACGG

Product: monooxygenase FAD-binding

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MADTLLDIPPVLIVGAGPTGLAAAMSLARARVPVRVIDKLAAPAPYSRAIGIQARTLELLEQHRAVEPFLALGHRAHAAA
LHADGRVIARLDFDPLQTRYPYLLLLDQSVTERLLAEHLAELGVTIERGVTLVECDTGGALLDVTLRDAGGRDERFSPSY
LIAADGAHSTVRHLLGIGFAGRAFEQTFLLADFAAIPDWPEDEIHLFTTPDGIAGLFPLGSGRYRLVADRPPVGDVSPDS
PPPSLADCEAVVRARIGASIAPSDLAWSSYFRLHSRMVERLRHGRVFFAGDAAHIHSPAGAQGMNTGVQEAFNLGWKLAR
VLAGGAPDRLLDTYHSERHPIERDVLRQTSLVTQIVEADHGAMKLLRDHLMPLLVSLGPLRDAVRRSVSELGVQYRKSPL
TLERVLDGGPRAGERAPDALLHVIDGPLGQAPGTARLYDVHDPASFTLLLLEAPPSTGLDDVPPLAADAQALIQRLERIM
PGAVRAWRVTDVEGDGADTLVQAYGRSRPAFYLLRPDGYIAARGRASSDANALLRHCETWFAGVPSPSA

Sequences:

>Translated_549_residues
MADTLLDIPPVLIVGAGPTGLAAAMSLARARVPVRVIDKLAAPAPYSRAIGIQARTLELLEQHRAVEPFLALGHRAHAAA
LHADGRVIARLDFDPLQTRYPYLLLLDQSVTERLLAEHLAELGVTIERGVTLVECDTGGALLDVTLRDAGGRDERFSPSY
LIAADGAHSTVRHLLGIGFAGRAFEQTFLLADFAAIPDWPEDEIHLFTTPDGIAGLFPLGSGRYRLVADRPPVGDVSPDS
PPPSLADCEAVVRARIGASIAPSDLAWSSYFRLHSRMVERLRHGRVFFAGDAAHIHSPAGAQGMNTGVQEAFNLGWKLAR
VLAGGAPDRLLDTYHSERHPIERDVLRQTSLVTQIVEADHGAMKLLRDHLMPLLVSLGPLRDAVRRSVSELGVQYRKSPL
TLERVLDGGPRAGERAPDALLHVIDGPLGQAPGTARLYDVHDPASFTLLLLEAPPSTGLDDVPPLAADAQALIQRLERIM
PGAVRAWRVTDVEGDGADTLVQAYGRSRPAFYLLRPDGYIAARGRASSDANALLRHCETWFAGVPSPSA
>Mature_548_residues
ADTLLDIPPVLIVGAGPTGLAAAMSLARARVPVRVIDKLAAPAPYSRAIGIQARTLELLEQHRAVEPFLALGHRAHAAAL
HADGRVIARLDFDPLQTRYPYLLLLDQSVTERLLAEHLAELGVTIERGVTLVECDTGGALLDVTLRDAGGRDERFSPSYL
IAADGAHSTVRHLLGIGFAGRAFEQTFLLADFAAIPDWPEDEIHLFTTPDGIAGLFPLGSGRYRLVADRPPVGDVSPDSP
PPSLADCEAVVRARIGASIAPSDLAWSSYFRLHSRMVERLRHGRVFFAGDAAHIHSPAGAQGMNTGVQEAFNLGWKLARV
LAGGAPDRLLDTYHSERHPIERDVLRQTSLVTQIVEADHGAMKLLRDHLMPLLVSLGPLRDAVRRSVSELGVQYRKSPLT
LERVLDGGPRAGERAPDALLHVIDGPLGQAPGTARLYDVHDPASFTLLLLEAPPSTGLDDVPPLAADAQALIQRLERIMP
GAVRAWRVTDVEGDGADTLVQAYGRSRPAFYLLRPDGYIAARGRASSDANALLRHCETWFAGVPSPSA

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=531, Percent_Identity=25.8003766478343, Blast_Score=109, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 59120; Mature: 58989

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTLLDIPPVLIVGAGPTGLAAAMSLARARVPVRVIDKLAAPAPYSRAIGIQARTLELL
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHCCCHHHHHHHH
EQHRAVEPFLALGHRAHAAALHADGRVIARLDFDPLQTRYPYLLLLDQSVTERLLAEHLA
HHHCCCCHHHHHCCHHHHHEEECCCEEEEEECCCCHHCCCCEEEEECHHHHHHHHHHHHH
ELGVTIERGVTLVECDTGGALLDVTLRDAGGRDERFSPSYLIAADGAHSTVRHLLGIGFA
HHCCCHHHCCEEEEECCCCEEEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCC
GRAFEQTFLLADFAAIPDWPEDEIHLFTTPDGIAGLFPLGSGRYRLVADRPPVGDVSPDS
CHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCCCCEEEEECCCCCCCCCCCC
PPPSLADCEAVVRARIGASIAPSDLAWSSYFRLHSRMVERLRHGRVFFAGDAAHIHSPAG
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCC
AQGMNTGVQEAFNLGWKLARVLAGGAPDRLLDTYHSERHPIERDVLRQTSLVTQIVEADH
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCH
GAMKLLRDHLMPLLVSLGPLRDAVRRSVSELGVQYRKSPLTLERVLDGGPRAGERAPDAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHH
LHVIDGPLGQAPGTARLYDVHDPASFTLLLLEAPPSTGLDDVPPLAADAQALIQRLERIM
HHHHCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHC
PGAVRAWRVTDVEGDGADTLVQAYGRSRPAFYLLRPDGYIAARGRASSDANALLRHCETW
CCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHH
FAGVPSPSA
HCCCCCCCC
>Mature Secondary Structure 
ADTLLDIPPVLIVGAGPTGLAAAMSLARARVPVRVIDKLAAPAPYSRAIGIQARTLELL
CCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHCCCHHHHHHHH
EQHRAVEPFLALGHRAHAAALHADGRVIARLDFDPLQTRYPYLLLLDQSVTERLLAEHLA
HHHCCCCHHHHHCCHHHHHEEECCCEEEEEECCCCHHCCCCEEEEECHHHHHHHHHHHHH
ELGVTIERGVTLVECDTGGALLDVTLRDAGGRDERFSPSYLIAADGAHSTVRHLLGIGFA
HHCCCHHHCCEEEEECCCCEEEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCC
GRAFEQTFLLADFAAIPDWPEDEIHLFTTPDGIAGLFPLGSGRYRLVADRPPVGDVSPDS
CHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCCCCEEEEECCCCCCCCCCCC
PPPSLADCEAVVRARIGASIAPSDLAWSSYFRLHSRMVERLRHGRVFFAGDAAHIHSPAG
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCC
AQGMNTGVQEAFNLGWKLARVLAGGAPDRLLDTYHSERHPIERDVLRQTSLVTQIVEADH
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCH
GAMKLLRDHLMPLLVSLGPLRDAVRRSVSELGVQYRKSPLTLERVLDGGPRAGERAPDAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHH
LHVIDGPLGQAPGTARLYDVHDPASFTLLLLEAPPSTGLDDVPPLAADAQALIQRLERIM
HHHHCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHC
PGAVRAWRVTDVEGDGADTLVQAYGRSRPAFYLLRPDGYIAARGRASSDANALLRHCETW
CCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHH
FAGVPSPSA
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]