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The map label for this gene is nuoF2 [H]

Identifier: 161525535

GI number: 161525535

Start: 2572780

End: 2574357

Strand: Direct

Name: nuoF2 [H]

Synonym: Bmul_2365

Alternate gene names: 161525535

Gene position: 2572780-2574357 (Clockwise)

Preceding gene: 161525534

Following gene: 161525536

Centisome position: 74.61

GC content: 70.41

Gene sequence:

>1578_bases
ATGACGACCCGTATCTACGTCCCGCGCGATTCGTCCGCGCTCGCACTCGGCGCCGACGCGCTCGCCGCCGCGATCGTGGC
CGAGGCCGAACGGCGCGGCGCCGCGATCGAACTCGTTCGCAACGGCTCGCGCGGGCTGCTGTGGCTCGAGCCGCTCGTCG
AAGTCGGCACCGCGGCCGGCCGCGTCGGCTATGCGAACCTGACGGCCGCCGACGTCCCCGCGCTGTTCGACGCCGGCTGG
CTCGAAGGCGGTGCGCATCCGAGCGGCGTCGGCCTCGTCGAAGCGTTGCCGTATCTCGCGCGCCAGCAGCGCCTGACGTT
CGCGCGCATCGGCATCACCGATCCGCTGTCGATCGACGACTATCTGAAATACGACGGCCTCGTCGGCCTGAAGAACGCGC
TCGCGCTCGACGGCGACGCCGCATGCGAGACGCTGATCGAGTCGGGCCTGCGCGGCCGCGGCGGCGCGGCGTTCCCCGCC
GGCATCAAATGGCGCACGGTGCGCCAGGCGAGCGCGAAACAGAAATACATCGTCTGCAACGCAGACGAAGGCGACTCGGG
CACGTTCTCCGATCGCCTGCTCATGGAATGCGATCCGTACTGCCTGATCGAAGGGATGATCATCGCCGGCGTCGCGACGG
GCGCGACGGTCGGCTACATCTACGTGCGCAGCGAATACCCGCACGCAATCGCGACGCTCGAAGCCGCGATCGCCCGCGCC
CGCGAAGCCGGCTGGCTCGGCGAGCACGTGCTCGGCTCCGCGCACGCATTCGAGCTGCACGTCGCGAAGGGCGCGGGCTC
GTACGTGTGCGGCGAGGAAACGGCGCTGCTCGAATCGCTCGAAGGCAAGCGCGGCGTCGTGCGCGCGAAGCCGCCGCTGC
CCGCGCTCGCGGGCCTGTTCGGCCAGCCGACCGTCATCAACAACGTGATCACGCTCGCCACCGCTCCGGTGATCTTCGCG
CGCGGCGCGGCGTTCTATCGCGATTACGGGATGGGCCGCTCGCGCGGCACGCTGCCGTTCCAGCTCGCCGGCAACATCCG
CTACGGCGGCCTCGTCGAACTCGCGTTCGGCGTCACGCTGCGCGAGCTGCTGTTCGACTTCGGCGGCGGCACCGCGACCG
GCCGCCCCGCGCGCGCCGCGCAGGTCGGCGGCCCGCTCGGCACCTATCTGCCCGAGCATCAGTGGGACGTGCCGCTCGAC
TACGAGGCGTATACGGCGATCGGCGCGGTCGTCGGTCACGGCGGCATCGTGCTGCACGACGACACGTCGAATCTCGCGGA
GCTCGCCGAATACGCGATGAAGTTCTGCGCGATCGAATCGTGCGGCAAATGCACGCCGTGCCGGATCGGTTCGACGCGCG
GCGTCGAGACGATCGCGCGCATCCGCGGCGGCGACACGTCCGAACGGCAGATCACGCTGCTGCGCGACCTGTGCGACACG
ATGCTCGCCGGCTCGCTGTGCGCGATGGGCGGGATGACGCCGTATCCGGTGCTGTCCGCGCTCGACCATTTCCCCGAAGA
TTTCGGGCTCGCCGCCGGCACGGATGCCGCGTCGAAGCCCGTCAAGGCTGCGGCCTGA

Upstream 100 bases:

>100_bases
CGCCGTCGATGACGGTCAACGGCGTGCTCCACGCGAAGGTCACGCCGGAAAAATTCGATGCGTTGCTCGCCGACGCGGTC
GCGCACGCCGGGGAGGCCGC

Downstream 100 bases:

>100_bases
GCCAGAAGGAGCCCTGCATGTCCCTCGACACGAACAACGTCCGCCAAGGCGGCTGCGGCTCGGGCCAATGCGCTTGCAAG
AGTGCCGCTCAGGCGCGCAC

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MTTRIYVPRDSSALALGADALAAAIVAEAERRGAAIELVRNGSRGLLWLEPLVEVGTAAGRVGYANLTAADVPALFDAGW
LEGGAHPSGVGLVEALPYLARQQRLTFARIGITDPLSIDDYLKYDGLVGLKNALALDGDAACETLIESGLRGRGGAAFPA
GIKWRTVRQASAKQKYIVCNADEGDSGTFSDRLLMECDPYCLIEGMIIAGVATGATVGYIYVRSEYPHAIATLEAAIARA
REAGWLGEHVLGSAHAFELHVAKGAGSYVCGEETALLESLEGKRGVVRAKPPLPALAGLFGQPTVINNVITLATAPVIFA
RGAAFYRDYGMGRSRGTLPFQLAGNIRYGGLVELAFGVTLRELLFDFGGGTATGRPARAAQVGGPLGTYLPEHQWDVPLD
YEAYTAIGAVVGHGGIVLHDDTSNLAELAEYAMKFCAIESCGKCTPCRIGSTRGVETIARIRGGDTSERQITLLRDLCDT
MLAGSLCAMGGMTPYPVLSALDHFPEDFGLAAGTDAASKPVKAAA

Sequences:

>Translated_525_residues
MTTRIYVPRDSSALALGADALAAAIVAEAERRGAAIELVRNGSRGLLWLEPLVEVGTAAGRVGYANLTAADVPALFDAGW
LEGGAHPSGVGLVEALPYLARQQRLTFARIGITDPLSIDDYLKYDGLVGLKNALALDGDAACETLIESGLRGRGGAAFPA
GIKWRTVRQASAKQKYIVCNADEGDSGTFSDRLLMECDPYCLIEGMIIAGVATGATVGYIYVRSEYPHAIATLEAAIARA
REAGWLGEHVLGSAHAFELHVAKGAGSYVCGEETALLESLEGKRGVVRAKPPLPALAGLFGQPTVINNVITLATAPVIFA
RGAAFYRDYGMGRSRGTLPFQLAGNIRYGGLVELAFGVTLRELLFDFGGGTATGRPARAAQVGGPLGTYLPEHQWDVPLD
YEAYTAIGAVVGHGGIVLHDDTSNLAELAEYAMKFCAIESCGKCTPCRIGSTRGVETIARIRGGDTSERQITLLRDLCDT
MLAGSLCAMGGMTPYPVLSALDHFPEDFGLAAGTDAASKPVKAAA
>Mature_524_residues
TTRIYVPRDSSALALGADALAAAIVAEAERRGAAIELVRNGSRGLLWLEPLVEVGTAAGRVGYANLTAADVPALFDAGWL
EGGAHPSGVGLVEALPYLARQQRLTFARIGITDPLSIDDYLKYDGLVGLKNALALDGDAACETLIESGLRGRGGAAFPAG
IKWRTVRQASAKQKYIVCNADEGDSGTFSDRLLMECDPYCLIEGMIIAGVATGATVGYIYVRSEYPHAIATLEAAIARAR
EAGWLGEHVLGSAHAFELHVAKGAGSYVCGEETALLESLEGKRGVVRAKPPLPALAGLFGQPTVINNVITLATAPVIFAR
GAAFYRDYGMGRSRGTLPFQLAGNIRYGGLVELAFGVTLRELLFDFGGGTATGRPARAAQVGGPLGTYLPEHQWDVPLDY
EAYTAIGAVVGHGGIVLHDDTSNLAELAEYAMKFCAIESCGKCTPCRIGSTRGVETIARIRGGDTSERQITLLRDLCDTM
LAGSLCAMGGMTPYPVLSALDHFPEDFGLAAGTDAASKPVKAAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=428, Percent_Identity=35.2803738317757, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI20149568, Length=363, Percent_Identity=38.2920110192837, Blast_Score=254, Evalue=1e-67,
Organism=Escherichia coli, GI1788620, Length=407, Percent_Identity=39.5577395577396, Blast_Score=290, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17531727, Length=422, Percent_Identity=36.7298578199052, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI19920794, Length=391, Percent_Identity=37.3401534526854, Blast_Score=258, Evalue=1e-68,
Organism=Drosophila melanogaster, GI45550452, Length=363, Percent_Identity=38.5674931129477, Blast_Score=243, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24653819, Length=348, Percent_Identity=31.8965517241379, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24653817, Length=332, Percent_Identity=29.5180722891566, Blast_Score=156, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55036; Mature: 54905

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRIYVPRDSSALALGADALAAAIVAEAERRGAAIELVRNGSRGLLWLEPLVEVGTAAG
CCCEEEECCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHCCCCC
RVGYANLTAADVPALFDAGWLEGGAHPSGVGLVEALPYLARQQRLTFARIGITDPLSIDD
CCCCCCCCHHHCCHHHHCHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHH
YLKYDGLVGLKNALALDGDAACETLIESGLRGRGGAAFPAGIKWRTVRQASAKQKYIVCN
HHHHCCCCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHCCCCEEEEEE
ADEGDSGTFSDRLLMECDPYCLIEGMIIAGVATGATVGYIYVRSEYPHAIATLEAAIARA
CCCCCCCCCCCCEEECCCCHHHCCCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHH
REAGWLGEHVLGSAHAFELHVAKGAGSYVCGEETALLESLEGKRGVVRAKPPLPALAGLF
HHCCCCHHHHHCCCEEEEEEEECCCCCEECCCHHHHHHHCCCCCCEEECCCCCHHHHHHC
GQPTVINNVITLATAPVIFARGAAFYRDYGMGRSRGTLPFQLAGNIRYGGLVELAFGVTL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
RELLFDFGGGTATGRPARAAQVGGPLGTYLPEHQWDVPLDYEAYTAIGAVVGHGGIVLHD
HHHHHHCCCCCCCCCCCCHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEC
DTSNLAELAEYAMKFCAIESCGKCTPCRIGSTRGVETIARIRGGDTSERQITLLRDLCDT
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHH
MLAGSLCAMGGMTPYPVLSALDHFPEDFGLAAGTDAASKPVKAAA
HHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTRIYVPRDSSALALGADALAAAIVAEAERRGAAIELVRNGSRGLLWLEPLVEVGTAAG
CCEEEECCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHCCCCC
RVGYANLTAADVPALFDAGWLEGGAHPSGVGLVEALPYLARQQRLTFARIGITDPLSIDD
CCCCCCCCHHHCCHHHHCHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHH
YLKYDGLVGLKNALALDGDAACETLIESGLRGRGGAAFPAGIKWRTVRQASAKQKYIVCN
HHHHCCCCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHCCCCEEEEEE
ADEGDSGTFSDRLLMECDPYCLIEGMIIAGVATGATVGYIYVRSEYPHAIATLEAAIARA
CCCCCCCCCCCCEEECCCCHHHCCCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHH
REAGWLGEHVLGSAHAFELHVAKGAGSYVCGEETALLESLEGKRGVVRAKPPLPALAGLF
HHCCCCHHHHHCCCEEEEEEEECCCCCEECCCHHHHHHHCCCCCCEEECCCCCHHHHHHC
GQPTVINNVITLATAPVIFARGAAFYRDYGMGRSRGTLPFQLAGNIRYGGLVELAFGVTL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
RELLFDFGGGTATGRPARAAQVGGPLGTYLPEHQWDVPLDYEAYTAIGAVVGHGGIVLHD
HHHHHHCCCCCCCCCCCCHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEC
DTSNLAELAEYAMKFCAIESCGKCTPCRIGSTRGVETIARIRGGDTSERQITLLRDLCDT
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHH
MLAGSLCAMGGMTPYPVLSALDHFPEDFGLAAGTDAASKPVKAAA
HHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]