Click here to switch to the map view.

The map label for this gene is cls [H]

Identifier: 161525462

GI number: 161525462

Start: 2505426

End: 2506856

Strand: Direct

Name: cls [H]

Synonym: Bmul_2292

Alternate gene names: 161525462

Gene position: 2505426-2506856 (Clockwise)

Preceding gene: 161525461

Following gene: 161525463

Centisome position: 72.65

GC content: 67.16

Gene sequence:

>1431_bases
GTGTCCGACCCCAACGGGCTGACATGGGCAGCCACCCTCGTATCGCTGGCGGTCGCTATCCCCACAGCGGGGCACGCGGT
CATCTACAAGCGTGACCCTCGATCGGCCACGCTGTGGGTGCTGCTGATCGCGCTGTTGCCGCTGGGCGGTTCGCTGCTGT
ATGGGCTGTTCGGCATCAACCGTTACGAGCGGCGGGCGCGGCGGCTGTTTCCGGGGACAGATCCTGCTGTTCGGCAGGAC
CTCCGCGCCTACGATGCCCAGGCCGTATCGGCGCCGTTTGCCGGCCTGGCGCACCTGGTGGGACGTGCCACCGGCCAGTC
GCTGACCAGCGGCAACGGCATCGAGCCGCTCGTCGATGGCGAGCAGGCCTACCCGGCCATGCTCGCTGCCATCGAGTCGG
CGCGGCACAGCGTCGCGCTGGCTTCGTACATCTTCGACAGCCAAGGCATCGGGGCGCAGTTCGTCGATGCGCTGCGCCGG
GCTCATGAGCGCGGCGTGCAAGTGCGGGTGCTGATCGACGACGTCTATGCCCGCTGGAGGCCCCGCAGCGCCTACCGCGC
CTTGCAATGCGCCGGCGTTCCGGCGGCGACGTTCAACCCGACGTTGATTCCCGCGCGCCTGCATGCCGCGCATCTGCGCA
ATCACCGCAAGCTGCTGGTGATCGATGGCGAGACGGGTTTCACCGGCGGCATGAACATCTTCAGCCCGTATTGGCGGCCC
GATGCGCCGGAGCAGGCCTGTCACGATTTGCACTTTCGTCTGCGCGGGCCGGTGGTTGCGCATCTGATGCGGTGCTTCAC
CGACGATTGGTGCGATACCACGGGCGAGCGGCTCAACGAGGGTCTCTGGGGCGAACCGCCCGCAACGGCTGATGAGCAAG
GAACCTCTTGGGCGCGCGGCATCGAGGCCGGTCCCGATGAGGCCCTGGACCGGCTGCGCTGGACCTTCATGGGCGCTTTG
AGCGCGGCGAAGCATTCGGTGCGCATCTGGACACCTTACTTCGTGCCCGATCAGCCGATGATCGCGGCGCTGAGCACGGC
CGCGTTGCGCGGCGTGCGCGTCGAGGTGCTGACGCCCGCAAACGGCGACCATCCCACGGTGCAATGGGCCGCGCGCGCCC
ACTACTGGCAGGTGCTGGAGCACGGTGTGCGCATCTTCGAACGGCCTGGCCCGTTCGACCACAGCAAGCTGATGCTGGTA
GACGGAGCGTGGTGCTGCCTGGGTTCGGCCAATTGGGATGCGCGCAGTTTGCGCCTCAACTTCGAGTTCAATGTGGAGGT
GTACGACACCGCGTTGTCTACGCGGCTGTCATCCCTTTTTGATGCCGCTCGTGATGCGTCGGGCGAGATATCGGCGAAGG
CGTTGCGCGCCCGCCCGCTGGCCATCCGCTTGCGCGACGGGGTGGCCCGTCTGTTCACCCCCATTCTCTAG

Upstream 100 bases:

>100_bases
ATGCCGCGAACGTGGCCGAGACGCCGCCGAGCGACGCGAGCAAGCCTGCGCGCAAGAAGAAGTCAGCGGGCGGCGAATCT
TGAGTCATGGGGAGGCTGTC

Downstream 100 bases:

>100_bases
CAACACGACTTGCGAGGAACATTGCCCATGGAAAAAAAGGATCAATGGAACATTGGCTACTGGATCGTCGCCGGCCTGTT
GCTGCTGACGCTGCAGAACT

Product: phospholipase D/transphosphatidylase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSDPNGLTWAATLVSLAVAIPTAGHAVIYKRDPRSATLWVLLIALLPLGGSLLYGLFGINRYERRARRLFPGTDPAVRQD
LRAYDAQAVSAPFAGLAHLVGRATGQSLTSGNGIEPLVDGEQAYPAMLAAIESARHSVALASYIFDSQGIGAQFVDALRR
AHERGVQVRVLIDDVYARWRPRSAYRALQCAGVPAATFNPTLIPARLHAAHLRNHRKLLVIDGETGFTGGMNIFSPYWRP
DAPEQACHDLHFRLRGPVVAHLMRCFTDDWCDTTGERLNEGLWGEPPATADEQGTSWARGIEAGPDEALDRLRWTFMGAL
SAAKHSVRIWTPYFVPDQPMIAALSTAALRGVRVEVLTPANGDHPTVQWAARAHYWQVLEHGVRIFERPGPFDHSKLMLV
DGAWCCLGSANWDARSLRLNFEFNVEVYDTALSTRLSSLFDAARDASGEISAKALRARPLAIRLRDGVARLFTPIL

Sequences:

>Translated_476_residues
MSDPNGLTWAATLVSLAVAIPTAGHAVIYKRDPRSATLWVLLIALLPLGGSLLYGLFGINRYERRARRLFPGTDPAVRQD
LRAYDAQAVSAPFAGLAHLVGRATGQSLTSGNGIEPLVDGEQAYPAMLAAIESARHSVALASYIFDSQGIGAQFVDALRR
AHERGVQVRVLIDDVYARWRPRSAYRALQCAGVPAATFNPTLIPARLHAAHLRNHRKLLVIDGETGFTGGMNIFSPYWRP
DAPEQACHDLHFRLRGPVVAHLMRCFTDDWCDTTGERLNEGLWGEPPATADEQGTSWARGIEAGPDEALDRLRWTFMGAL
SAAKHSVRIWTPYFVPDQPMIAALSTAALRGVRVEVLTPANGDHPTVQWAARAHYWQVLEHGVRIFERPGPFDHSKLMLV
DGAWCCLGSANWDARSLRLNFEFNVEVYDTALSTRLSSLFDAARDASGEISAKALRARPLAIRLRDGVARLFTPIL
>Mature_475_residues
SDPNGLTWAATLVSLAVAIPTAGHAVIYKRDPRSATLWVLLIALLPLGGSLLYGLFGINRYERRARRLFPGTDPAVRQDL
RAYDAQAVSAPFAGLAHLVGRATGQSLTSGNGIEPLVDGEQAYPAMLAAIESARHSVALASYIFDSQGIGAQFVDALRRA
HERGVQVRVLIDDVYARWRPRSAYRALQCAGVPAATFNPTLIPARLHAAHLRNHRKLLVIDGETGFTGGMNIFSPYWRPD
APEQACHDLHFRLRGPVVAHLMRCFTDDWCDTTGERLNEGLWGEPPATADEQGTSWARGIEAGPDEALDRLRWTFMGALS
AAKHSVRIWTPYFVPDQPMIAALSTAALRGVRVEVLTPANGDHPTVQWAARAHYWQVLEHGVRIFERPGPFDHSKLMLVD
GAWCCLGSANWDARSLRLNFEFNVEVYDTALSTRLSSLFDAARDASGEISAKALRARPLAIRLRDGVARLFTPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=487, Percent_Identity=29.1581108829569, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787007, Length=336, Percent_Identity=31.547619047619, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 52321; Mature: 52190

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPNGLTWAATLVSLAVAIPTAGHAVIYKRDPRSATLWVLLIALLPLGGSLLYGLFGIN
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHCCC
RYERRARRLFPGTDPAVRQDLRAYDAQAVSAPFAGLAHLVGRATGQSLTSGNGIEPLVDG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
EQAYPAMLAAIESARHSVALASYIFDSQGIGAQFVDALRRAHERGVQVRVLIDDVYARWR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEEHHHHHCC
PRSAYRALQCAGVPAATFNPTLIPARLHAAHLRNHRKLLVIDGETGFTGGMNIFSPYWRP
CHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCCEEEEEECCCCCCCCCHHCCCCCCC
DAPEQACHDLHFRLRGPVVAHLMRCFTDDWCDTTGERLNEGLWGEPPATADEQGTSWARG
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCHHHHC
IEAGPDEALDRLRWTFMGALSAAKHSVRIWTPYFVPDQPMIAALSTAALRGVRVEVLTPA
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECEECCCCHHHHHHHHHHHCCEEEEEECCC
NGDHPTVQWAARAHYWQVLEHGVRIFERPGPFDHSKLMLVDGAWCCLGSANWDARSLRLN
CCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEECCCCCCCEEEEEE
FEFNVEVYDTALSTRLSSLFDAARDASGEISAKALRARPLAIRLRDGVARLFTPIL
EEEEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEHHHHHHHHHCCC
>Mature Secondary Structure 
SDPNGLTWAATLVSLAVAIPTAGHAVIYKRDPRSATLWVLLIALLPLGGSLLYGLFGIN
CCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHCCC
RYERRARRLFPGTDPAVRQDLRAYDAQAVSAPFAGLAHLVGRATGQSLTSGNGIEPLVDG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
EQAYPAMLAAIESARHSVALASYIFDSQGIGAQFVDALRRAHERGVQVRVLIDDVYARWR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEEHHHHHCC
PRSAYRALQCAGVPAATFNPTLIPARLHAAHLRNHRKLLVIDGETGFTGGMNIFSPYWRP
CHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCCEEEEEECCCCCCCCCHHCCCCCCC
DAPEQACHDLHFRLRGPVVAHLMRCFTDDWCDTTGERLNEGLWGEPPATADEQGTSWARG
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCHHHHC
IEAGPDEALDRLRWTFMGALSAAKHSVRIWTPYFVPDQPMIAALSTAALRGVRVEVLTPA
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECEECCCCHHHHHHHHHHHCCEEEEEECCC
NGDHPTVQWAARAHYWQVLEHGVRIFERPGPFDHSKLMLVDGAWCCLGSANWDARSLRLN
CCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEECCCCCCCEEEEEE
FEFNVEVYDTALSTRLSSLFDAARDASGEISAKALRARPLAIRLRDGVARLFTPIL
EEEEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA