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The map label for this gene is pilR [H]

Identifier: 161524897

GI number: 161524897

Start: 1904454

End: 1905848

Strand: Reverse

Name: pilR [H]

Synonym: Bmul_1724

Alternate gene names: 161524897

Gene position: 1905848-1904454 (Counterclockwise)

Preceding gene: 161524898

Following gene: 161524896

Centisome position: 55.27

GC content: 65.88

Gene sequence:

>1395_bases
ATGAGAACTCCCACCCCCGCAATCGAAGAACTCGACCTCTACGTCTGGGAAGGCAAGGCGGACATCGTCGACCGCGTCGC
CCGATGCATGGCGAGCTTCGACGTCGAAGTGATCCGCGCCGACAACGCGGAGATCTCGCCCGAACGCGCCGCGTTGCGGC
CGTCGCTCGCGATCATCAGCGTGACGATGATCGAAAGCGGCGCGGCGTTCCTGCGCGACTGGCAAGCCAACATCGGCATG
CCGGTCGTCTGGGTCGGCGCCGCGCGCGATCACGACGCGTCGCAGTATCCGCCCGAGTACTCGCATATCCTCCCGCTCGA
CTTCACGTGCGCGGAACTGCGCGGCATGGTCGGCAAGCTCGTCACGCAATTGCGCGCGCACGCCGCCGAAACGCTGCAGC
CGTCGGTGCTCGTCGCGCACTCGGAATCGATGCAGGCGCTGCTGCGCGAAGTCGATACCTTCGCCGATTGCGATACGAAC
GTGCTGCTGCACGGCGAGACCGGCGTCGGCAAGGAACGCATCGCGCAGTTGCTGCACGAAAAGCATTCGCGCTATCGGCA
CGGCGAATTCGTGCCGGTCAACTGCGGCGCGATTCCGGACGGCCTGTTCGAATCGCTGTTCTTCGGGCACGCGAAAGGCT
CGTTCACCGGCGCGGTTGTTGCGCATAAAGGCTATTTCGAGCAGGCCGCCGGCGGCACGCTGTTTCTCGACGAAGTCGGC
GATCTGCCGCTCTATCAGCAAGTGAAGTTGCTGCGCGTGCTCGAAGACGGTGCGGTGCTGCGTGTCGGCGCGACGTCGCC
CGTCAAGGTCGATTTCCGGCTCGTCGCGGCGAGCAACAAGAAGCTGCCGCAGCTCGTGAAGGACGGGCTGTTCCGCGCGG
ATCTCTATTACCGGCTCGCGGTGATCGAGCTGAACATCCCGTCGCTCGAAGAGCGCGGGGCGGTCGACAAGATCGCGCTG
TTCAAGTCGTTCGTCGCGGAGGTGGTCGGCGAGGCGCGGCTCGCGGAATTGCCCGATCTGCCGTACTGGCTGGCCGACGC
GGTCGCGGACAGCTATTTCCCCGGCAATGTCCGCGAACTGCGCAATCTGGCCGAGCGCGTCGGCGTGACGGTGCGGCAGA
CGGGCGGCTGGGACGCCGCGCGGCTGCAGCGCTTGATCGCGCACGCACGCAGCGCCGCGCAGCCGGTGCCGGCGGAAAGC
GCGGCGGAGGTGTTCGTCGATCGCAGCAAGTGGGACATGAACGAGCGCAACCGCGTGATCGCCGCGCTCGACGCGAACGG
CTGGCGGCGCCAGGATACCGCGCAGCAACTGGGGATCAGCCGCAAGGTGCTGTGGGAAAAAATGCGGAAGTACCAGATAT
TCGACGAAGAGCCCGAGACCCGCGAAAGCGAGTAA

Upstream 100 bases:

>100_bases
GGGCGTACAAATCGGTGTGGCGACCGTCCACAATGAGGCGTTGACTTGTGGTATTCCACAACTGGTCAGCAACACCGGAA
ATTGATGCTTTGCCTAAAGA

Downstream 100 bases:

>100_bases
TTAATGAGATAAAATCGCGCTGAATTACAACAACTCGCGCGGGGAATAACACTTGAAGAGCCATATCACGAGGTTGGGGC
GGGCGTTCGTATTGCTCGCC

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MRTPTPAIEELDLYVWEGKADIVDRVARCMASFDVEVIRADNAEISPERAALRPSLAIISVTMIESGAAFLRDWQANIGM
PVVWVGAARDHDASQYPPEYSHILPLDFTCAELRGMVGKLVTQLRAHAAETLQPSVLVAHSESMQALLREVDTFADCDTN
VLLHGETGVGKERIAQLLHEKHSRYRHGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVG
DLPLYQQVKLLRVLEDGAVLRVGATSPVKVDFRLVAASNKKLPQLVKDGLFRADLYYRLAVIELNIPSLEERGAVDKIAL
FKSFVAEVVGEARLAELPDLPYWLADAVADSYFPGNVRELRNLAERVGVTVRQTGGWDAARLQRLIAHARSAAQPVPAES
AAEVFVDRSKWDMNERNRVIAALDANGWRRQDTAQQLGISRKVLWEKMRKYQIFDEEPETRESE

Sequences:

>Translated_464_residues
MRTPTPAIEELDLYVWEGKADIVDRVARCMASFDVEVIRADNAEISPERAALRPSLAIISVTMIESGAAFLRDWQANIGM
PVVWVGAARDHDASQYPPEYSHILPLDFTCAELRGMVGKLVTQLRAHAAETLQPSVLVAHSESMQALLREVDTFADCDTN
VLLHGETGVGKERIAQLLHEKHSRYRHGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVG
DLPLYQQVKLLRVLEDGAVLRVGATSPVKVDFRLVAASNKKLPQLVKDGLFRADLYYRLAVIELNIPSLEERGAVDKIAL
FKSFVAEVVGEARLAELPDLPYWLADAVADSYFPGNVRELRNLAERVGVTVRQTGGWDAARLQRLIAHARSAAQPVPAES
AAEVFVDRSKWDMNERNRVIAALDANGWRRQDTAQQLGISRKVLWEKMRKYQIFDEEPETRESE
>Mature_464_residues
MRTPTPAIEELDLYVWEGKADIVDRVARCMASFDVEVIRADNAEISPERAALRPSLAIISVTMIESGAAFLRDWQANIGM
PVVWVGAARDHDASQYPPEYSHILPLDFTCAELRGMVGKLVTQLRAHAAETLQPSVLVAHSESMQALLREVDTFADCDTN
VLLHGETGVGKERIAQLLHEKHSRYRHGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVG
DLPLYQQVKLLRVLEDGAVLRVGATSPVKVDFRLVAASNKKLPQLVKDGLFRADLYYRLAVIELNIPSLEERGAVDKIAL
FKSFVAEVVGEARLAELPDLPYWLADAVADSYFPGNVRELRNLAERVGVTVRQTGGWDAARLQRLIAHARSAAQPVPAES
AAEVFVDRSKWDMNERNRVIAALDANGWRRQDTAQQLGISRKVLWEKMRKYQIFDEEPETRESE

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=325, Percent_Identity=37.5384615384615, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1790437, Length=354, Percent_Identity=35.5932203389831, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1790299, Length=440, Percent_Identity=32.9545454545455, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1788550, Length=317, Percent_Identity=35.3312302839117, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1789233, Length=324, Percent_Identity=34.5679012345679, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789087, Length=326, Percent_Identity=36.5030674846626, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI87081872, Length=324, Percent_Identity=35.1851851851852, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI87082152, Length=258, Percent_Identity=40.6976744186046, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1788905, Length=233, Percent_Identity=40.7725321888412, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1786524, Length=334, Percent_Identity=35.9281437125749, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1787583, Length=340, Percent_Identity=33.5294117647059, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789828, Length=212, Percent_Identity=36.3207547169811, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI87081858, Length=297, Percent_Identity=29.96632996633, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51526; Mature: 51526

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTPTPAIEELDLYVWEGKADIVDRVARCMASFDVEVIRADNAEISPERAALRPSLAIIS
CCCCCCCHHHCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHCCCEEEEE
VTMIESGAAFLRDWQANIGMPVVWVGAARDHDASQYPPEYSHILPLDFTCAELRGMVGKL
EEEHHCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VTQLRAHAAETLQPSVLVAHSESMQALLREVDTFADCDTNVLLHGETGVGKERIAQLLHE
HHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
KHSRYRHGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVG
HHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHCCCCEEEECCCC
DLPLYQQVKLLRVLEDGAVLRVGATSPVKVDFRLVAASNKKLPQLVKDGLFRADLYYRLA
CCCHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCCHHHHHCCCHHHHHEEEEE
VIELNIPSLEERGAVDKIALFKSFVAEVVGEARLAELPDLPYWLADAVADSYFPGNVREL
EEEECCCCCHHCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHCCCCCCHHHH
RNLAERVGVTVRQTGGWDAARLQRLIAHARSAAQPVPAESAAEVFVDRSKWDMNERNRVI
HHHHHHHCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCEE
AALDANGWRRQDTAQQLGISRKVLWEKMRKYQIFDEEPETRESE
EEECCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRTPTPAIEELDLYVWEGKADIVDRVARCMASFDVEVIRADNAEISPERAALRPSLAIIS
CCCCCCCHHHCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHCCCEEEEE
VTMIESGAAFLRDWQANIGMPVVWVGAARDHDASQYPPEYSHILPLDFTCAELRGMVGKL
EEEHHCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VTQLRAHAAETLQPSVLVAHSESMQALLREVDTFADCDTNVLLHGETGVGKERIAQLLHE
HHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
KHSRYRHGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVG
HHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHCCCCEEEECCCC
DLPLYQQVKLLRVLEDGAVLRVGATSPVKVDFRLVAASNKKLPQLVKDGLFRADLYYRLA
CCCHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCCHHHHHCCCHHHHHEEEEE
VIELNIPSLEERGAVDKIALFKSFVAEVVGEARLAELPDLPYWLADAVADSYFPGNVREL
EEEECCCCCHHCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHCCCCCCHHHH
RNLAERVGVTVRQTGGWDAARLQRLIAHARSAAQPVPAESAAEVFVDRSKWDMNERNRVI
HHHHHHHCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCEE
AALDANGWRRQDTAQQLGISRKVLWEKMRKYQIFDEEPETRESE
EEECCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]