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The map label for this gene is kgtP [H]

Identifier: 161524795

GI number: 161524795

Start: 1793735

End: 1795039

Strand: Reverse

Name: kgtP [H]

Synonym: Bmul_1622

Alternate gene names: 161524795

Gene position: 1795039-1793735 (Counterclockwise)

Preceding gene: 161524796

Following gene: 161524794

Centisome position: 52.05

GC content: 66.28

Gene sequence:

>1305_bases
ATGGACGTCCAGACCGACCAAGCCGCGCTGTCCGCGCACGATACCCGGCGGCGCGTGTTCGCGATCGTCGGCGCCTCGTC
GGGCAACCTCGTCGAGTGGTTCGACTTCTACATTTATTCGTTCTGCGCGCTGTACTTCGCGCCCGCGTTCTTCCCGAGCG
GCAATACGACGACGCAGCTGCTGAACACGGCCGGCGTGTTCGCGGCCGGCTTCCTGATGCGGCCGATCGGCGGCTGGCTG
TTCGGCCGCATCGCCGACCGCCACGGCCGCCGCACCGCGATGATGATCTCGGTGCTGATGATGTGCGGCGGCTCGCTCGT
GATTGCCGTGCTGCCGACCTACGCGCAGATCGGCGCGCTCGCGCCGGCGCTGCTGCTCGTCGCGCGCCTGTTCCAGGGGC
TGTCGGTCGGCGGCGAATACGGGACGAGCGCGACGTACATGAGCGAGGTCGCGCTGAAGGGGCGGCGCGGGTTCTTCGCG
TCGTTCCAGTACGTGACGCTGATCGGCGGCCAGCTGTGCGCGCTGCTCGTGCTCGTGATCCTGCAGCAGACGCTGTCGCA
CGACGAACTGAAGGCGTGGGGCTGGCGCATTCCGTTCGTCGTCGGGGCGGCGGCCGCGCTGATCTCGCTCTATCTGCGCA
AGTCGCTCGACGAAACCTCGACGAACGAGTCGCGCGACAAGAAGGATGCGGGCACGATCCGCGGCGTCTGGCAGCACAAG
GGCGCCTTCTTCACGGTGGTCGGCTTCACGGCCGGCGGCTCGCTGATCTTCTACACGTTCACGACCTACATGCAGAAGTA
CCTCGTGAACACGGCCGGCATGCATGCGAAGACGGCCAGCAACGTGATGACCGTCGCGCTGCTCGTCTACATGCTGATGC
AGCCGCTGTTCGGCGCGCTGTCGGACAAGATCGGCCGACGCATGTCGATGATCCTGTTCGGCTCGTTCGCCGTGATCGGC
ACGGTGCCGCTGATGCATGCGCTGAAGGGCGTGACGAGCCCGGTCGCCGCGTTCGCGCTGATCACGGTCGCGCTCGCGAT
CGTCAGCTTCTACACGTCGATCAGCGGCCTGATCAAGGCCGAGATGTTCCCGCCCGAAGTGCGCGCGATGGGCGTCGGCC
TGTCGTATGCGGTCGCGAACGCGATCTTCGGCGGCTCGGCGGAATACGTCGCGCTGTGGTTCAAGTCGATCGGCAGCGAG
GAGACGTTCTACTGGTACGTGACCGCGCTGTGCGCGATCTCGCTGATCGTGTCGTGGCGGATGCGCGATCCGAGCAAGGA
AGGGTATCTGCGTCACGAGCCGTGA

Upstream 100 bases:

>100_bases
ATCGCGTAGCCCGTGCACCCGGAACCGGTGCCACGGCGACCGCACGCGGCGGCACCCCGGCCGCCTCTCTCTGCAAGCAC
TATAAGGAACAGACCAACCC

Downstream 100 bases:

>100_bases
TCGGCGCCGCGCGGCGGGCTTGCGCGCCCGTCGCGACCGGCCGCGATACCGGCAGCGCCCCGTCATTGCGGCGGGGCGTT
TTTTTGCGCGCTCGCGCGTT

Product: major facilitator superfamily metabolite/H(+) symporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MDVQTDQAALSAHDTRRRVFAIVGASSGNLVEWFDFYIYSFCALYFAPAFFPSGNTTTQLLNTAGVFAAGFLMRPIGGWL
FGRIADRHGRRTAMMISVLMMCGGSLVIAVLPTYAQIGALAPALLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFA
SFQYVTLIGGQLCALLVLVILQQTLSHDELKAWGWRIPFVVGAAAALISLYLRKSLDETSTNESRDKKDAGTIRGVWQHK
GAFFTVVGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTVALLVYMLMQPLFGALSDKIGRRMSMILFGSFAVIG
TVPLMHALKGVTSPVAAFALITVALAIVSFYTSISGLIKAEMFPPEVRAMGVGLSYAVANAIFGGSAEYVALWFKSIGSE
ETFYWYVTALCAISLIVSWRMRDPSKEGYLRHEP

Sequences:

>Translated_434_residues
MDVQTDQAALSAHDTRRRVFAIVGASSGNLVEWFDFYIYSFCALYFAPAFFPSGNTTTQLLNTAGVFAAGFLMRPIGGWL
FGRIADRHGRRTAMMISVLMMCGGSLVIAVLPTYAQIGALAPALLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFA
SFQYVTLIGGQLCALLVLVILQQTLSHDELKAWGWRIPFVVGAAAALISLYLRKSLDETSTNESRDKKDAGTIRGVWQHK
GAFFTVVGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTVALLVYMLMQPLFGALSDKIGRRMSMILFGSFAVIG
TVPLMHALKGVTSPVAAFALITVALAIVSFYTSISGLIKAEMFPPEVRAMGVGLSYAVANAIFGGSAEYVALWFKSIGSE
ETFYWYVTALCAISLIVSWRMRDPSKEGYLRHEP
>Mature_434_residues
MDVQTDQAALSAHDTRRRVFAIVGASSGNLVEWFDFYIYSFCALYFAPAFFPSGNTTTQLLNTAGVFAAGFLMRPIGGWL
FGRIADRHGRRTAMMISVLMMCGGSLVIAVLPTYAQIGALAPALLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFA
SFQYVTLIGGQLCALLVLVILQQTLSHDELKAWGWRIPFVVGAAAALISLYLRKSLDETSTNESRDKKDAGTIRGVWQHK
GAFFTVVGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTVALLVYMLMQPLFGALSDKIGRRMSMILFGSFAVIG
TVPLMHALKGVTSPVAAFALITVALAIVSFYTSISGLIKAEMFPPEVRAMGVGLSYAVANAIFGGSAEYVALWFKSIGSE
ETFYWYVTALCAISLIVSWRMRDPSKEGYLRHEP

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=422, Percent_Identity=70.1421800947867, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1790550, Length=424, Percent_Identity=29.2452830188679, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1788292, Length=435, Percent_Identity=28.735632183908, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1789941, Length=424, Percent_Identity=31.1320754716981, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI87082231, Length=328, Percent_Identity=27.1341463414634, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI87082404, Length=313, Percent_Identity=24.9201277955272, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47195; Mature: 47195

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCC
>Mature Secondary Structure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CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]