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The map label for this gene is mexA [H]

Identifier: 161524789

GI number: 161524789

Start: 1787448

End: 1788641

Strand: Reverse

Name: mexA [H]

Synonym: Bmul_1616

Alternate gene names: 161524789

Gene position: 1788641-1787448 (Counterclockwise)

Preceding gene: 161524792

Following gene: 161524788

Centisome position: 51.87

GC content: 72.95

Gene sequence:

>1194_bases
ATGAATGACAAACGCTCGCTGTCGCGCCGGCTGACGCCGCTCGCGCTGGCCCTCGCCGTGGCCGCGGCCGGCTGCGGAAA
GGGCAAACCGGATGCCGAGCCGCCGGCCGTGCAGAAGGCGACGGTCGTCACGGTGCGTCCGGTCGCGGTGCCGATGACCG
TCGAGCTGCCGGGCCGGCTCGATGCCTATCGACGCGCGGAAGTGCGCGCGCGCGTCGCCGGCATCGTTACGGCACGCACG
TACGACGAAGGCCAGGAAGTGAAGCAGGGCGCCGTGCTGTTTCGCATCGATCCCGCACCGTTGAAGGCGGCACGCGATGC
GGCGCAGGGCGCGCTCGCCAAGGCGCAGGCCGCGGCGGCCGCGGCCGCCGACAAGCGGCGGCGCTATGCCGATCTCGTGC
GCGATCACGCCGTCAGCGAGCGCGACTACACCGAGGCCGTCGCGGCCGACACGCAGGCGAAGGCCGAAGTCGCGTCGGCG
AAGGCCGAACTCGCGCGTGCGCAACTGCAGCTCGACTACGCGAACGTGACCGCGCCGATTTCCGGGCGGGCGCGGCGCGC
GCTCGTGACCGAAGGCGCGCTCGTCGGGCAGGGCGAAGCGACGCCGCTCACGACCGTCGAGCAGCTCGATCCGATCTACG
TGAATTTCTCGCAGCCGGCCGCCGACGTCGAGGCGCTGCGGCGCGCCGTGAAAAGCGGGCGCGCGACCGGCATCGCGCAG
CAGGACGTCGCGGTGACGCTGCTGCGCGCCGACGGCACGCCGTATCCGCTGAAGGGCAAGCTGCTGTTCAGCGATCTCGC
GGTCGATCCGACCACCGATACCGTCGCGATGCGCGCGCTGTTTCCGAATCCCGAGCGCGAACTGCTGCCGGGCGCGTACG
TGCGGATCGCGCTCGATGCGGCGGTCGACCACCGCGCGCTGCTCGTGCCGCGCGACGCGCTGCTGCGTACGGCCGAGCGC
ACGTCGGTGCGCGTCGTCGATGCGGCGGGCAGCGTGAAGGACGTCGAAGTCGTCGCGGACCGCATGAGCGGCCGCGACTG
GCGCATCACGCGCGGGCTGAACGGCGGCGAGCGCGTGATCGTCGACAACGCCGCGCAGTTCGCCCCCGGCACCGTCGTGA
AGCCCGTCGACGCGCGGCCCGCGCAGGCGGCGCCGGATTCGGCGGCCTCCGGCACGCCCGGCCGTCAAACCTGA

Upstream 100 bases:

>100_bases
ATTGGATGGAGGATCGGTGTATTTAATCAGTCGTGACTGATTATAATCGGCGACCCTGAGCCGCCGCATTGTATCGGCGG
CGCACGATCCGAGGTCCCAG

Downstream 100 bases:

>100_bases
CCGGTAGCGCTGACCATGGCACGTTTCTTCATCGATCGCCCCGTATTCGCGTGGGTGATCGCACTCTTCATCATGCTGGG
CGGCGCGTTCGCGATCCGCG

Product: periplasmic multidrug efflux lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNDKRSLSRRLTPLALALAVAAAGCGKGKPDAEPPAVQKATVVTVRPVAVPMTVELPGRLDAYRRAEVRARVAGIVTART
YDEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAAAAAADKRRRYADLVRDHAVSERDYTEAVAADTQAKAEVASA
KAELARAQLQLDYANVTAPISGRARRALVTEGALVGQGEATPLTTVEQLDPIYVNFSQPAADVEALRRAVKSGRATGIAQ
QDVAVTLLRADGTPYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALDAAVDHRALLVPRDALLRTAER
TSVRVVDAAGSVKDVEVVADRMSGRDWRITRGLNGGERVIVDNAAQFAPGTVVKPVDARPAQAAPDSAASGTPGRQT

Sequences:

>Translated_397_residues
MNDKRSLSRRLTPLALALAVAAAGCGKGKPDAEPPAVQKATVVTVRPVAVPMTVELPGRLDAYRRAEVRARVAGIVTART
YDEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAAAAAADKRRRYADLVRDHAVSERDYTEAVAADTQAKAEVASA
KAELARAQLQLDYANVTAPISGRARRALVTEGALVGQGEATPLTTVEQLDPIYVNFSQPAADVEALRRAVKSGRATGIAQ
QDVAVTLLRADGTPYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALDAAVDHRALLVPRDALLRTAER
TSVRVVDAAGSVKDVEVVADRMSGRDWRITRGLNGGERVIVDNAAQFAPGTVVKPVDARPAQAAPDSAASGTPGRQT
>Mature_397_residues
MNDKRSLSRRLTPLALALAVAAAGCGKGKPDAEPPAVQKATVVTVRPVAVPMTVELPGRLDAYRRAEVRARVAGIVTART
YDEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAAAAAADKRRRYADLVRDHAVSERDYTEAVAADTQAKAEVASA
KAELARAQLQLDYANVTAPISGRARRALVTEGALVGQGEATPLTTVEQLDPIYVNFSQPAADVEALRRAVKSGRATGIAQ
QDVAVTLLRADGTPYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALDAAVDHRALLVPRDALLRTAER
TSVRVVDAAGSVKDVEVVADRMSGRDWRITRGLNGGERVIVDNAAQFAPGTVVKPVDARPAQAAPDSAASGTPGRQT

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=381, Percent_Identity=41.4698162729659, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI1786668, Length=374, Percent_Identity=40.6417112299465, Blast_Score=240, Evalue=9e-65,
Organism=Escherichia coli, GI1789929, Length=388, Percent_Identity=37.3711340206186, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI87082036, Length=356, Percent_Identity=30.8988764044944, Blast_Score=138, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41859; Mature: 41859

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKRSLSRRLTPLALALAVAAAGCGKGKPDAEPPAVQKATVVTVRPVAVPMTVELPGRL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECEEEEEEEECCCCH
DAYRRAEVRARVAGIVTARTYDEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAAA
HHHHHHHHHHHHHHEEEEEECCCCHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
AAADKRRRYADLVRDHAVSERDYTEAVAADTQAKAEVASAKAELARAQLQLDYANVTAPI
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCEECCC
SGRARRALVTEGALVGQGEATPLTTVEQLDPIYVNFSQPAADVEALRRAVKSGRATGIAQ
CCCHHEEEEECCCEECCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCC
QDVAVTLLRADGTPYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALDA
CCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHCCCEEEEEEEEC
AVDHRALLVPRDALLRTAERTSVRVVDAAGSVKDVEVVADRMSGRDWRITRGLNGGERVI
CCCCCEEEECHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEE
VDNAAQFAPGTVVKPVDARPAQAAPDSAASGTPGRQT
EECCHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNDKRSLSRRLTPLALALAVAAAGCGKGKPDAEPPAVQKATVVTVRPVAVPMTVELPGRL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECEEEEEEEECCCCH
DAYRRAEVRARVAGIVTARTYDEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAAA
HHHHHHHHHHHHHHEEEEEECCCCHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
AAADKRRRYADLVRDHAVSERDYTEAVAADTQAKAEVASAKAELARAQLQLDYANVTAPI
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCEECCC
SGRARRALVTEGALVGQGEATPLTTVEQLDPIYVNFSQPAADVEALRRAVKSGRATGIAQ
CCCHHEEEEECCCEECCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCC
QDVAVTLLRADGTPYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALDA
CCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHCCCEEEEEEEEC
AVDHRALLVPRDALLRTAERTSVRVVDAAGSVKDVEVVADRMSGRDWRITRGLNGGERVI
CCCCCEEEECHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEE
VDNAAQFAPGTVVKPVDARPAQAAPDSAASGTPGRQT
EECCHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]