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The map label for this gene is cobW [H]

Identifier: 161524756

GI number: 161524756

Start: 1736568

End: 1737677

Strand: Reverse

Name: cobW [H]

Synonym: Bmul_1583

Alternate gene names: 161524756

Gene position: 1737677-1736568 (Counterclockwise)

Preceding gene: 161524757

Following gene: 161524755

Centisome position: 50.39

GC content: 68.02

Gene sequence:

>1110_bases
ATGACCCTACGCAAGCTGCCCGTCACGATCGTCACGGGCTTTCTCGGCAGCGGCAAGACCACGCTGATGCGCCACATCCT
GCAGCAGGCGAACGGCCGCCGCATCGCGGTCATCGTCAACGAGTTCGGCGAACTCGGCATCGACGGCGAAATCCTGAAAG
GCTGCGGCATCGGTTGCGACGACGCGGCCGGCGAGGCGGCCGGGCAACTGTACGAACTCGCGAACGGCTGCCTCTGCTGC
ACCGTGCAGGAGGAGTTCTATCCGGTGATGGAAGCGCTGCTCGAACGGCGCGCGGACATCGATCACGTGCTGATCGAAAC
CTCGGGCCTCGCGCTGCCGAAGCCGCTCGTGCAGGCGTTCAACTGGCCGACGATCCGCCACGCGTTCACGGTCGATGCGG
TCGTGACCGTCGTCGACGGGCCCGCGGCCGCAAGCGGCCAGTTCGCGGAAAACCCGCAGGCCGTCGACGCGCAGCGGCGC
GCGGATCCGAACCTCGATCACGAATCGCCGTTGCACGAGCTGTTCGCCGATCAGCTGTCGGCGGCCGATCTCGTGATCGT
GAACAAGGCCGAGCTCGTCGACGATGCGCAGTTCGCGCAGATCGAAGCCGCGATCCGCGACGAGATCCCGCCGCAGGTGA
AGATCGTGCGCGCGACGCGCGGCGAGCTCGATCTCGCGATGCTGATCGGGCTCGAATCGGCGTCCGAAGACACGATCCAT
TTGCGTCACGACCATCACGGTGCGGCCGGCGTAGGTGCGACCGGCGTAGGTGCGGCCGACGACGAAGAGCACGACCATCA
TCACGACGATTTCGATTCGGTCGTCGTGAGCGGCGACGCGGGCACGCGCGAGGCGACGATCGCCGCGCTGCAGCGGCTCG
TCGAAACGCACACGATCTATCGCGCGAAGGGATTCGCGGCGCTGCCCGACGCGCCGATGCGGCTCGTGATCCAGGGCGTG
GGCCGCCGCTTCGACAGCTATTTCGACCGCCGCTGGCAGGACGGCGAAGCGCGCGCGAGCCGCTTCGTGCTGATCGGCGA
ACGGCTCGACGCGGCCGAGCTGCAACGCGCGTTCGACGCCGCGTGCGCGGCCGAGCCGCAGCAGGCGTAA

Upstream 100 bases:

>100_bases
GCGTGCTGGGCCGTGTCGGCCGCGATCTACCGCTTCAAACGCTACGACGAGATCGACGTGACGATCTCCGCGTGATGCCT
TTTTCGACCGAGGACCTACC

Downstream 100 bases:

>100_bases
CGCGCGATGCATCTGCTGCGCACCACGCCGGGCGGCTTCGTCGACGACACGCAGGGTGTCGTGCGCATCGATCAGCGCCC
GGCCGACATCGTGATCCTGA

Product: cobalamin biosynthesis protein CobW

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTLRKLPVTIVTGFLGSGKTTLMRHILQQANGRRIAVIVNEFGELGIDGEILKGCGIGCDDAAGEAAGQLYELANGCLCC
TVQEEFYPVMEALLERRADIDHVLIETSGLALPKPLVQAFNWPTIRHAFTVDAVVTVVDGPAAASGQFAENPQAVDAQRR
ADPNLDHESPLHELFADQLSAADLVIVNKAELVDDAQFAQIEAAIRDEIPPQVKIVRATRGELDLAMLIGLESASEDTIH
LRHDHHGAAGVGATGVGAADDEEHDHHHDDFDSVVVSGDAGTREATIAALQRLVETHTIYRAKGFAALPDAPMRLVIQGV
GRRFDSYFDRRWQDGEARASRFVLIGERLDAAELQRAFDAACAAEPQQA

Sequences:

>Translated_369_residues
MTLRKLPVTIVTGFLGSGKTTLMRHILQQANGRRIAVIVNEFGELGIDGEILKGCGIGCDDAAGEAAGQLYELANGCLCC
TVQEEFYPVMEALLERRADIDHVLIETSGLALPKPLVQAFNWPTIRHAFTVDAVVTVVDGPAAASGQFAENPQAVDAQRR
ADPNLDHESPLHELFADQLSAADLVIVNKAELVDDAQFAQIEAAIRDEIPPQVKIVRATRGELDLAMLIGLESASEDTIH
LRHDHHGAAGVGATGVGAADDEEHDHHHDDFDSVVVSGDAGTREATIAALQRLVETHTIYRAKGFAALPDAPMRLVIQGV
GRRFDSYFDRRWQDGEARASRFVLIGERLDAAELQRAFDAACAAEPQQA
>Mature_368_residues
TLRKLPVTIVTGFLGSGKTTLMRHILQQANGRRIAVIVNEFGELGIDGEILKGCGIGCDDAAGEAAGQLYELANGCLCCT
VQEEFYPVMEALLERRADIDHVLIETSGLALPKPLVQAFNWPTIRHAFTVDAVVTVVDGPAAASGQFAENPQAVDAQRRA
DPNLDHESPLHELFADQLSAADLVIVNKAELVDDAQFAQIEAAIRDEIPPQVKIVRATRGELDLAMLIGLESASEDTIHL
RHDHHGAAGVGATGVGAADDEEHDHHHDDFDSVVVSGDAGTREATIAALQRLVETHTIYRAKGFAALPDAPMRLVIQGVG
RRFDSYFDRRWQDGEARASRFVLIGERLDAAELQRAFDAACAAEPQQA

Specific function: Might be involved in cobalt reduction leading to cobalt(I) corrinoids [H]

COG id: COG0523

COG function: function code R; Putative GTPases (G3E family)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cobW C-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI33469141, Length=379, Percent_Identity=26.9129287598945, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI126722884, Length=379, Percent_Identity=26.6490765171504, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI148727351, Length=379, Percent_Identity=26.6490765171504, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI146231952, Length=379, Percent_Identity=26.3852242744063, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI223941779, Length=372, Percent_Identity=27.4193548387097, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI223941776, Length=380, Percent_Identity=25.2631578947368, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI119120938, Length=151, Percent_Identity=37.0860927152318, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI87082430, Length=369, Percent_Identity=26.8292682926829, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788499, Length=185, Percent_Identity=32.4324324324324, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324356, Length=371, Percent_Identity=24.7978436657682, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012824
- InterPro:   IPR003495
- InterPro:   IPR011629 [H]

Pfam domain/function: PF02492 cobW; PF07683 CobW_C [H]

EC number: NA

Molecular weight: Translated: 39845; Mature: 39714

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRKLPVTIVTGFLGSGKTTLMRHILQQANGRRIAVIVNEFGELGIDGEILKGCGIGCD
CCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEECHHCCCCCCHHHHHCCCCCC
DAAGEAAGQLYELANGCLCCTVQEEFYPVMEALLERRADIDHVLIETSGLALPKPLVQAF
CCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHC
NWPTIRHAFTVDAVVTVVDGPAAASGQFAENPQAVDAQRRADPNLDHESPLHELFADQLS
CCCHHHHHEEEEEEEEEECCCCCCCCCCCCCCCHHCHHHCCCCCCCCCCHHHHHHHHHHC
AADLVIVNKAELVDDAQFAQIEAAIRDEIPPQVKIVRATRGELDLAMLIGLESASEDTIH
CCCEEEEECHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCCCEEE
LRHDHHGAAGVGATGVGAADDEEHDHHHDDFDSVVVSGDAGTREATIAALQRLVETHTIY
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
RAKGFAALPDAPMRLVIQGVGRRFDSYFDRRWQDGEARASRFVLIGERLDAAELQRAFDA
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEEECCCCHHHHHHHHHH
ACAAEPQQA
HHCCCCCCC
>Mature Secondary Structure 
TLRKLPVTIVTGFLGSGKTTLMRHILQQANGRRIAVIVNEFGELGIDGEILKGCGIGCD
CCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEECHHCCCCCCHHHHHCCCCCC
DAAGEAAGQLYELANGCLCCTVQEEFYPVMEALLERRADIDHVLIETSGLALPKPLVQAF
CCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHC
NWPTIRHAFTVDAVVTVVDGPAAASGQFAENPQAVDAQRRADPNLDHESPLHELFADQLS
CCCHHHHHEEEEEEEEEECCCCCCCCCCCCCCCHHCHHHCCCCCCCCCCHHHHHHHHHHC
AADLVIVNKAELVDDAQFAQIEAAIRDEIPPQVKIVRATRGELDLAMLIGLESASEDTIH
CCCEEEEECHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCCCEEE
LRHDHHGAAGVGATGVGAADDEEHDHHHDDFDSVVVSGDAGTREATIAALQRLVETHTIY
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
RAKGFAALPDAPMRLVIQGVGRRFDSYFDRRWQDGEARASRFVLIGERLDAAELQRAFDA
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEEECCCCHHHHHHHHHH
ACAAEPQQA
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]