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The map label for this gene is gcl [H]

Identifier: 161524569

GI number: 161524569

Start: 1538517

End: 1540292

Strand: Reverse

Name: gcl [H]

Synonym: Bmul_1396

Alternate gene names: 161524569

Gene position: 1540292-1538517 (Counterclockwise)

Preceding gene: 161524580

Following gene: 161524568

Centisome position: 44.67

GC content: 66.61

Gene sequence:

>1776_bases
ATGGCCAAAATGAGAGCTGTCGACGCCGCCGTGCTCGTGCTCGAGAAGGAAGGCATCCAGACCGCGTTCGGCGTGCCCGG
CGCCGCGATCAACCCGTTCTACTCGGCGATGCGCAAGTCGGGCGGCATCAGCCACGTGCTGGCGCGCCACGTCGAAGGCG
CGTCGCACATGGCCGAAGGCTTCACGCGCGCGGCACCGGGCAACATCGGCGTGTGCATCGGCACGTCGGGCCCGGCCGGC
ACCGACATGATCACGGGCCTCTACTCCGCCTCGGCCGACTCGATTCCGATTCTCGCGATCACCGGCCAGGCGCCGCGCGC
GCGTCTCTACAAGGAAGACTTCCAGGCCGTCGACATCGAGTCGATCGCGAAGCCCGTCACCAAGTGGGCCGTCACCGTGC
GCGAGCCGGCGCTCGTGCCGCGCGTGTTCCAGCAGGCATTCCACCTGATGCGCTCGGGCCGTCCGGGCCCGGTGCTGATC
GATCTGCCGATCGACGTGCAACTCGCCGAAATCGAATTCGACATCGACACGTACGAACCGCTGCCGGTCTACAAGCCCGC
GGCGACCCGCGCGCAGATCGAGAAGGCGCTCGCGATGCTCAACGACGCGGACAAGCCGCTGATCGTGTCGGGCGGCGGCG
TGCTCAACGCGGCCGCCGAAGACCTGCTCGTGCAGTTCGCGGAAACCGTCGGCGTGCCGGTGATTCCGACGCTGATGTCG
TGGGGCGCGATTCCGGACGACCATCCGCTGATGGCCGGCATGGTCGGCCTGCAGACGTCGCACCGCTACGGCAACGCGAC
GATGCTCGCCTCGGACTTCGTGCTCGGCATCGGCAACCGGTGGGCGAACCGCCACACCGGCAGCATCGACGTCTACACGA
AGGGCCGCAAGTTCGTGCACGTCGACATCGAACCGACGCAGATCGGCCGCGTGTTCGGCCCCGATCTCGGGATCGTGTCG
GACGCGAAGGCGGCGCTCGAGCTGTTCGTCGCGGTCGCGCAGGAATGGAAGGCGGCCGGCAAGCTGAAGGACCGCAGCGC
CTGGGTGGCCGAGTGTCAGCAGCGCAAGCGCACGCTGCAGCGCAAGACACACTTCGAGAACGTGCCGATCAAGCCGCAGC
GCGTGTACGAAGAGATGAACAAGGTGTTCGGCCGCGATACGTGCTACGTGAGCACGATCGGTCTGTCGCAGATCGCCGCC
GCGCAGTTCCTGCACGTGTTCAAGGCGCGCAACTGGATCAACTGCGGCCAGGCCGGCCCGCTCGGCTGGACGATTCCCGC
CGCGCTCGGCGTGCGTGCGGCCGATCCGGCGCGCCCGATCGTCGCGCTGTCCGGCGACTACGACTTCCAGTTCATGATCG
AGGAACTGGCAGCCGGCGCGCAGTTCAAGCTGCCGTACGTGCACGTGGTCGTGAACAACTCGTACCTCGGGCTGATCCGT
CAGGCGCAGCGCGCGTTCGACATGGACTACTGCGTGCAGCTCGCGTTCGACAACGTGAACGCACCGGAGCTGAACGGCTA
TGGCGTCGATCACGTCGCCGTGGCCGAAGGCCTCGGCTGCAAGGCGCTGCGCGTGTTCAAGCCGGAAGAGATCGAACCGG
CGCTGAAGCAGGCGCAATCGCTCGCGGAAGAGTTCAGCGTGCCGGTGATCGTCGAAGTGATCCTCGAGCGCGTGACGAAC
ATCTCGATGGGCACCGAGATCGATGCGATCAACGAATTCGAGGACCTCGCGGAGAAAGCCGAACACGCGCCGACCGCCAT
CTCGATGCTCGACTGA

Upstream 100 bases:

>100_bases
CGACTTCGGCTGATCTTATCAAACCTTTCCTGCATCCCTAAAGTGTCCTCAACAGACCGATTACCGAGATTCACCCCATT
TCAGGTTCAAGGAGACATTC

Downstream 100 bases:

>100_bases
TCCGCGGGATTTTTTTGACTGACCGACCGAAGAGGCTTAGCTCATGCCGAAGTTTGCTGCAAACCTGACCATGCTGTTCA
ACGAAGTGCCGTTCCTCGAC

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MAKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEGFTRAAPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLI
DLPIDVQLAEIEFDIDTYEPLPVYKPAATRAQIEKALAMLNDADKPLIVSGGGVLNAAAEDLLVQFAETVGVPVIPTLMS
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSIDVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVAVAQEWKAAGKLKDRSAWVAECQQRKRTLQRKTHFENVPIKPQRVYEEMNKVFGRDTCYVSTIGLSQIAA
AQFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPARPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVFKPEEIEPALKQAQSLAEEFSVPVIVEVILERVTN
ISMGTEIDAINEFEDLAEKAEHAPTAISMLD

Sequences:

>Translated_591_residues
MAKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEGFTRAAPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLI
DLPIDVQLAEIEFDIDTYEPLPVYKPAATRAQIEKALAMLNDADKPLIVSGGGVLNAAAEDLLVQFAETVGVPVIPTLMS
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSIDVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVAVAQEWKAAGKLKDRSAWVAECQQRKRTLQRKTHFENVPIKPQRVYEEMNKVFGRDTCYVSTIGLSQIAA
AQFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPARPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVFKPEEIEPALKQAQSLAEEFSVPVIVEVILERVTN
ISMGTEIDAINEFEDLAEKAEHAPTAISMLD
>Mature_590_residues
AKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEGFTRAAPGNIGVCIGTSGPAGT
DMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLID
LPIDVQLAEIEFDIDTYEPLPVYKPAATRAQIEKALAMLNDADKPLIVSGGGVLNAAAEDLLVQFAETVGVPVIPTLMSW
GAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSIDVYTKGRKFVHVDIEPTQIGRVFGPDLGIVSD
AKAALELFVAVAQEWKAAGKLKDRSAWVAECQQRKRTLQRKTHFENVPIKPQRVYEEMNKVFGRDTCYVSTIGLSQIAAA
QFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPARPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIRQ
AQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVFKPEEIEPALKQAQSLAEEFSVPVIVEVILERVTNI
SMGTEIDAINEFEDLAEKAEHAPTAISMLD

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=23.3096085409253, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI21361361, Length=595, Percent_Identity=23.8655462184874, Blast_Score=113, Evalue=5e-25,
Organism=Escherichia coli, GI1786717, Length=590, Percent_Identity=78.6440677966102, Blast_Score=982, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=544, Percent_Identity=34.0073529411765, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1790104, Length=483, Percent_Identity=33.7474120082816, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=25.7713248638838, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1788716, Length=477, Percent_Identity=25.3668763102725, Blast_Score=134, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17542570, Length=554, Percent_Identity=24.9097472924188, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17531299, Length=511, Percent_Identity=22.5048923679061, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17531301, Length=511, Percent_Identity=22.5048923679061, Blast_Score=98, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=598, Percent_Identity=30.4347826086957, Blast_Score=263, Evalue=8e-71,
Organism=Saccharomyces cerevisiae, GI6320816, Length=564, Percent_Identity=24.468085106383, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=25.5244755244755, Blast_Score=148, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 63993; Mature: 63862

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEG
CCCCHHHCEEEEEEECCCCCHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
FTRAAPGNIGVCIGTSGPAGTDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLIDLPIDVQLAEIEFDIDTYEP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCC
LPVYKPAATRAQIEKALAMLNDADKPLIVSGGGVLNAAAEDLLVQFAETVGVPVIPTLMS
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSIDVYTKGRKFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEHHHHHEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVAVAQEWKAAGKLKDRSAWVAECQQRKRTLQ
EECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKTHFENVPIKPQRVYEEMNKVFGRDTCYVSTIGLSQIAAAQFLHVFKARNWINCGQAGP
HHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPARPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCEEECCEEEEEECCCHHHHHH
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVFKPEEIEPALKQAQS
HHHHHHCHHHHHHEEECCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCHHHHHHHH
LAEEFSVPVIVEVILERVTNISMGTEIDAINEFEDLAEKAEHAPTAISMLD
HHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure 
AKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEG
CCCHHHCEEEEEEECCCCCHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
FTRAAPGNIGVCIGTSGPAGTDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLIDLPIDVQLAEIEFDIDTYEP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCC
LPVYKPAATRAQIEKALAMLNDADKPLIVSGGGVLNAAAEDLLVQFAETVGVPVIPTLMS
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSIDVYTKGRKFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEHHHHHEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVAVAQEWKAAGKLKDRSAWVAECQQRKRTLQ
EECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKTHFENVPIKPQRVYEEMNKVFGRDTCYVSTIGLSQIAAAQFLHVFKARNWINCGQAGP
HHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPARPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCEEECCEEEEEECCCHHHHHH
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVFKPEEIEPALKQAQS
HHHHHHCHHHHHHEEECCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCHHHHHHHH
LAEEFSVPVIVEVILERVTNISMGTEIDAINEFEDLAEKAEHAPTAISMLD
HHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]