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The map label for this gene is rluF [H]

Identifier: 161524427

GI number: 161524427

Start: 1380989

End: 1381738

Strand: Reverse

Name: rluF [H]

Synonym: Bmul_1254

Alternate gene names: 161524427

Gene position: 1381738-1380989 (Counterclockwise)

Preceding gene: 161524428

Following gene: 161524421

Centisome position: 40.07

GC content: 68.67

Gene sequence:

>750_bases
ATGCGCCTGTCGAAGCGGATGTCGGAGCTCGGCCTCTGCTCGCGCCGCGAAGCCGACGAATGGATCGAGAAAGGCTGGGT
GCTCGTCGACGGCGAGCGCATCGACACGCTCGGCACGAAGGTCCGCGCCGATCAGCGCATCGAGATCGACGAGCGCGCGC
AGGCCGCGCAGGCCGCGCAAGTGACGATCCTGCTGCACAAGCCGGTCGGTTACGTGTCCGGCCAGGCCGAAGACGGCTAC
GAGCCGGCCAGCGTCCTGATCACGCGTGAAAACCACTGGAGCGGCGACCGTTCGCCGCTGCGCTTCTCGCCGCAGCATCT
GCGCGCGCTCGCGCCGGCCGGCCGGCTCGACATCGACTCGACGGGCCTGCTCGTGCTGACGCAGAACGGCCGGATCGCCA
AACAGCTGATCGGCGAACAGTCGGACGTCGACAAGGAATACCTGGTGCGCGTGCGCTTCGGCGACCGTCTGACCGACATC
GACCAGCACTTCCCGGCCGAACGGCTCGCGCAGCTGCGGCACGGCCTCGAGCTCGACGGCGTCGCACTGAAGCCCGCGAT
GGTGAGCTGGCAAAACGGCGAGCAGCTGCGCTTCGTGCTGCGCGAAGGGCGCAAGCGTCAGATCCGCCGGATGTGCGAAC
TCGTCGGTCTCGAGGTGATCGGCCTCAAGCGCGTGCGGATGGGCCGCGTGACGCTCGGCGCGCTGCCGCAAGGCCAATGG
CGCTACCTGTCCGCCGACGAGCAGTTCTGA

Upstream 100 bases:

>100_bases
CACCGCCGAGCGCCGCCCGTCCGGCAACGCGCGACTGAAAACCACCGAACCGGTGAAGCGCCGCGCCGCGGACGTCGATC
GCGGCGACGAAGCCGGCCTG

Downstream 100 bases:

>100_bases
CCGGCACGGCGCGGCCCGCACCGCGCCGCGTGCACCGCATCAACGGAAAAGCCCGCCATCGACGGCGGGCTTTTTCTTTC
TCCGTTCATCACGACGCGCG

Product: RNA-binding S4 domain-containing protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLGTKVRADQRIEIDERAQAAQAAQVTILLHKPVGYVSGQAEDGY
EPASVLITRENHWSGDRSPLRFSPQHLRALAPAGRLDIDSTGLLVLTQNGRIAKQLIGEQSDVDKEYLVRVRFGDRLTDI
DQHFPAERLAQLRHGLELDGVALKPAMVSWQNGEQLRFVLREGRKRQIRRMCELVGLEVIGLKRVRMGRVTLGALPQGQW
RYLSADEQF

Sequences:

>Translated_249_residues
MRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLGTKVRADQRIEIDERAQAAQAAQVTILLHKPVGYVSGQAEDGY
EPASVLITRENHWSGDRSPLRFSPQHLRALAPAGRLDIDSTGLLVLTQNGRIAKQLIGEQSDVDKEYLVRVRFGDRLTDI
DQHFPAERLAQLRHGLELDGVALKPAMVSWQNGEQLRFVLREGRKRQIRRMCELVGLEVIGLKRVRMGRVTLGALPQGQW
RYLSADEQF
>Mature_249_residues
MRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLGTKVRADQRIEIDERAQAAQAAQVTILLHKPVGYVSGQAEDGY
EPASVLITRENHWSGDRSPLRFSPQHLRALAPAGRLDIDSTGLLVLTQNGRIAKQLIGEQSDVDKEYLVRVRFGDRLTDI
DQHFPAERLAQLRHGLELDGVALKPAMVSWQNGEQLRFVLREGRKRQIRRMCELVGLEVIGLKRVRMGRVTLGALPQGQW
RYLSADEQF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=249, Percent_Identity=35.3413654618474, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1788510, Length=248, Percent_Identity=29.0322580645161, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787524, Length=255, Percent_Identity=32.9411764705882, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 28271; Mature: 28271

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLGTKVRADQRIEIDERAQAAQAAQ
CCHHHHHHHHCCCCCCCHHHHHHCCEEEECCCEECHHCCHHCCCCCCCHHHHHHHHHHEE
VTILLHKPVGYVSGQAEDGYEPASVLITRENHWSGDRSPLRFSPQHLRALAPAGRLDIDS
EEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCEECC
TGLLVLTQNGRIAKQLIGEQSDVDKEYLVRVRFGDRLTDIDQHFPAERLAQLRHGLELDG
CCEEEEECCCCHHHHHCCCCCCCCHHHEEEEECCCCHHHHHHCCCHHHHHHHHCCCCCCC
VALKPAMVSWQNGEQLRFVLREGRKRQIRRMCELVGLEVIGLKRVRMGRVTLGALPQGQW
EEECCEEEECCCCHHHHHHHHHCHHHHHHHHHHHHCCHHCCHHHEEECCEEECCCCCCCE
RYLSADEQF
EEECCCCCC
>Mature Secondary Structure
MRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLGTKVRADQRIEIDERAQAAQAAQ
CCHHHHHHHHCCCCCCCHHHHHHCCEEEECCCEECHHCCHHCCCCCCCHHHHHHHHHHEE
VTILLHKPVGYVSGQAEDGYEPASVLITRENHWSGDRSPLRFSPQHLRALAPAGRLDIDS
EEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCEECC
TGLLVLTQNGRIAKQLIGEQSDVDKEYLVRVRFGDRLTDIDQHFPAERLAQLRHGLELDG
CCEEEEECCCCHHHHHCCCCCCCCHHHEEEEECCCCHHHHHHCCCHHHHHHHHCCCCCCC
VALKPAMVSWQNGEQLRFVLREGRKRQIRRMCELVGLEVIGLKRVRMGRVTLGALPQGQW
EEECCEEEECCCCHHHHHHHHHCHHHHHHHHHHHHCCHHCCHHHEEECCEEECCCCCCCE
RYLSADEQF
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]