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The map label for this gene is dmlR [H]

Identifier: 161524400

GI number: 161524400

Start: 1348862

End: 1349767

Strand: Direct

Name: dmlR [H]

Synonym: Bmul_1227

Alternate gene names: 161524400

Gene position: 1348862-1349767 (Clockwise)

Preceding gene: 161524397

Following gene: 161524401

Centisome position: 39.11

GC content: 69.65

Gene sequence:

>906_bases
ATGGACCGGTTCAAACAGATCGAAACGTTCGTCGCGGTCGCGGCGAAAGGCAGCCTGTCGGCGGCCGCGCACGCGGAGGG
CGTCGCGCCGGCGATCATCGGCCGCCGTCTCGACGCGCTCGAGGAGCGGCTCGGCGTCAAGCTGCTCGTGCGCACGACGC
GCAAGCTCACGCTGACCTTCGAGGGCTCCGCATTCCTCGAGGATTGCCAGCGCATCATCAACGACATGCAGAACGCGGAA
GCGAGCGTGTCCGCCGGCGGCGTGAAGGCGAGCGGCCATTTGCGCATCACGGCGCCCGCCGGCTTCGGCCGCCGCCACGT
CGCGCCGCTCGTGCCCGACTTCAGCGGCGCGCATCCGGACGTGTCGGTCACGCTCGATCTGTCGGACCGGATGGTCGACC
TCGTCAACGAAGGCTTCGATTGCGCGGTGCGGCTCGGCGAGCTGCCCGATTCGTCGCTCGTGTCGCTGAAGCTCGGCGAG
AACCGGCGCGTGTGCGTCGCGTCGCCCGCGTATCTCGCGCGGCGCGGCACGCCGACGACGCTCGCGGAACTCGCGCGCCA
CAACTGCCTCGCGCTCGCCGCGAACGCGAACCAGCAGCGCGGCTGGACATTCCAGGAAGACGACAAGGTCGTGTCGATCC
GCGTGAGCGGCACGATGGAGTGCTCCGACGGCGCGGTGCTGCACGAGTGGTGCCTGGCCGGCCACGGTCTGGCATGGCGA
TCGTGGTGGGAAGTCGGCGACGACATCGCGGCCGGCCGCCTCGTCAGCGTGCTGGACGCGTTCGCGGCGCCGCCGATCGG
CATTCATGCCGTATTTCCGCAGCGCCGGCACCTGCCGCTGCGCGTGCGGCTGTTTCTCGATTACCTGAAGCACACGTACG
AGCGGCCCGGTTACTGGGGCGAATAG

Upstream 100 bases:

>100_bases
AAAAAAGACGCTAATCATCACTTGATCTTTTACTTTCATATACCTAATCTTGAGCCGCTTCAGTCATCAGAGCGGGACGG
CGTGCCGTCCCTTTCGATTC

Downstream 100 bases:

>100_bases
CGTCGCGCGGCAACATGCGGGCCAATCCGCTGCCGCCGCTGCCGCGTCGCGCACTACAATCGAAACAGGCAGGCGCGCGG
CGTCGCGACGAGCAGGGACG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDRFKQIETFVAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRIINDMQNAE
ASVSAGGVKASGHLRITAPAGFGRRHVAPLVPDFSGAHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGE
NRRVCVASPAYLARRGTPTTLAELARHNCLALAANANQQRGWTFQEDDKVVSIRVSGTMECSDGAVLHEWCLAGHGLAWR
SWWEVGDDIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDYLKHTYERPGYWGE

Sequences:

>Translated_301_residues
MDRFKQIETFVAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRIINDMQNAE
ASVSAGGVKASGHLRITAPAGFGRRHVAPLVPDFSGAHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGE
NRRVCVASPAYLARRGTPTTLAELARHNCLALAANANQQRGWTFQEDDKVVSIRVSGTMECSDGAVLHEWCLAGHGLAWR
SWWEVGDDIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDYLKHTYERPGYWGE
>Mature_301_residues
MDRFKQIETFVAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRIINDMQNAE
ASVSAGGVKASGHLRITAPAGFGRRHVAPLVPDFSGAHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGE
NRRVCVASPAYLARRGTPTTLAELARHNCLALAANANQQRGWTFQEDDKVVSIRVSGTMECSDGAVLHEWCLAGHGLAWR
SWWEVGDDIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDYLKHTYERPGYWGE

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=290, Percent_Identity=35.1724137931034, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=31.4685314685315, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=32.8813559322034, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=30.6896551724138, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=30.0353356890459, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1787589, Length=287, Percent_Identity=30.6620209059233, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1789639, Length=299, Percent_Identity=28.7625418060201, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1786448, Length=279, Percent_Identity=26.8817204301075, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788706, Length=189, Percent_Identity=26.984126984127, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790011, Length=297, Percent_Identity=24.2424242424242, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789173, Length=250, Percent_Identity=27.6, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=26.027397260274, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32799; Mature: 32799

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00037 MYB_1 ; PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRFKQIETFVAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTF
CCHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEECEEEEEEE
EGSAFLEDCQRIINDMQNAEASVSAGGVKASGHLRITAPAGFGRRHVAPLVPDFSGAHPD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCC
VSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGENRRVCVASPAYLARRGTPTT
EEEEEECHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECCHHHHCCCCCHH
LAELARHNCLALAANANQQRGWTFQEDDKVVSIRVSGTMECSDGAVLHEWCLAGHGLAWR
HHHHHHCCEEEEEECCCCCCCCCCCCCCCEEEEEEECEEECCCCHHHHHHHHHCCCCCHH
SWWEVGDDIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDYLKHTYERPGYWG
HHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
E
C
>Mature Secondary Structure
MDRFKQIETFVAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTF
CCHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEECEEEEEEE
EGSAFLEDCQRIINDMQNAEASVSAGGVKASGHLRITAPAGFGRRHVAPLVPDFSGAHPD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCC
VSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGENRRVCVASPAYLARRGTPTT
EEEEEECHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECCHHHHCCCCCHH
LAELARHNCLALAANANQQRGWTFQEDDKVVSIRVSGTMECSDGAVLHEWCLAGHGLAWR
HHHHHHCCEEEEEECCCCCCCCCCCCCCCEEEEEEECEEECCCCHHHHHHHHHCCCCCHH
SWWEVGDDIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDYLKHTYERPGYWG
HHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]