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The map label for this gene is mls [H]

Identifier: 161524398

GI number: 161524398

Start: 1346284

End: 1347876

Strand: Reverse

Name: mls [H]

Synonym: Bmul_1225

Alternate gene names: 161524398

Gene position: 1347876-1346284 (Counterclockwise)

Preceding gene: 161524399

Following gene: 161524395

Centisome position: 39.09

GC content: 64.78

Gene sequence:

>1593_bases
ATGAGCACCCCGATCACGCTGCCGCAAGGCTTGGCGATCACCGGCGAGATCAAGCCGGGTTACGAAGCGATCCTGACGCC
CGACGCGCTCGAGCTCGTCGCTGCGCTGCATCGCGCGTTCGAACCGCGCCGCCAGGCGCTGCTGCAGGCGCGCGTCGAGC
GCACGAAGCGTCTCGACGCCGGCGAGCGCCCCGATTTTCTGCCCGAGACGAAGGCCATCCGCGAAGGCGACTGGAAGGTC
GCACCGCTGCCGGCCGACCTGCAGTGCCGCCGCGTCGAGATCACCGGCCCCGTCGAGCGCAAGATGATCATCAATGCGCT
GAACTCGGGCGCCGATTCGTACATGACGGACTTCGAGGATTCGAACGCGCCGAGCTGGACGAATCAGATCGACGGCCAGA
TCAACCTGAAGGACGCGGTGCGCCGCACGATCTCGCTCGAGCAGAACGGCAAGTCGTACAAGCTGAACGACAAGATCGCG
ACGCTAATCGTGCGTCCGCGCGGCTGGCACCTCGACGAGAAGCACGTGACGGTCGACGGCCAGCGTGTGTCCGGCGGCAT
CTTCGATTTCGCGCTGTTCCTGTTCCATAACGCGAAGGAGCTGATCGCGCGCGGTTCGGGCCCGTACTTCTACCTGCCGA
AGATGGAAAGCCACCTCGAGGCGCGCCTGTGGAACGACATCTTCGTCGCCGCGCAGGAAGCGATCGGCATTCCGCGCGGC
ACGATCCGCGCGACGGTGCTGATCGAGACGATCCTCGCCGCGTTCGAAATGGACGAGATCCTCTACGAGCTGCGCGAACA
CAGCTCGGGCCTGAACGCGGGCCGCTGGGACTACATTTTCTCGGCGATCAAGAAGTTCAAGAACGATCGCGACTTCTGCC
TCGCCGATCGTTCGAAGATCACGATGACGGTGCCGTTCATGCGCGCCTACGCGCTGCTGCTGCTGAAGACCTGCCACAAG
CGCAATGCGCCGGCGATCGGCGGGATGAGCGCGCTGATTCCGATCAAGAACGATCCGGAAGCGAACGAGAAGGCGATGGC
CGGCGTGCGTTCGGACAAGCAGCGCGATGCGACGGACGGCTACGACGGCGGCTGGGTGGCGCATCCGGGCCTCGTGCCGA
TCGCGATGGAGGAATTCGTCAAGGTGCTCGGCGACAAGCCGAACCAGATCGGCAAGCAGCGCGACGACGTGCAGGTCGAG
GGCAAGAACCTGCTCGACTTCCAGCCCGAGGCGCCGATCACCGAAGCGGGCCTGCGCAACAACATCAACGTCGGGATCCA
CTATCTCGGCGCGTGGCTCGACGGCAACGGCTGCGTGCCGATTCACAACCTGATGGAAGATGCGGCCACCGCCGAGATCT
CGCGCTCGCAGGTGTGGCAGTGGATCCGCTCGCCGAAGGGCGTGCTCGAGGACGGCCGCAAGGTCACCGCGGAACTCGTG
CGCGAGTTCGCGAAGGCCGAGCTCGAGAACGTGAAGCGCTCGGTCGGCGGCAACACGCAGCCTTACGAGCGTGCGGCGGC
GATCTTCGAACAGATGTCGACGTCGGAAGGCTTCACCGAATTCCTGACGCTGCCGCTGTACGAGGAAATCTGA

Upstream 100 bases:

>100_bases
GCGAACCGCACGCGCCCCGGCCCGGGCGCATGACGTTCGCATCGCTGCCGCCTTCCCCCTTCACCGAAACCGCAACTGAC
CGACCAAGGAGATGAGACCC

Downstream 100 bases:

>100_bases
TACGGCCGGCGGCCGCGGCGCACAGCCGCGGGCCGGCCGGTTCGTTCGTCAAAAGTCAAAAAGCGCCCTGCGGGGCGCTT
TCTTTTTTGCGACAGCCGGC

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MSTPITLPQGLAITGEIKPGYEAILTPDALELVAALHRAFEPRRQALLQARVERTKRLDAGERPDFLPETKAIREGDWKV
APLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFEDSNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYKLNDKIA
TLIVRPRGWHLDEKHVTVDGQRVSGGIFDFALFLFHNAKELIARGSGPYFYLPKMESHLEARLWNDIFVAAQEAIGIPRG
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLADRSKITMTVPFMRAYALLLLKTCHK
RNAPAIGGMSALIPIKNDPEANEKAMAGVRSDKQRDATDGYDGGWVAHPGLVPIAMEEFVKVLGDKPNQIGKQRDDVQVE
GKNLLDFQPEAPITEAGLRNNINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLEDGRKVTAELV
REFAKAELENVKRSVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI

Sequences:

>Translated_530_residues
MSTPITLPQGLAITGEIKPGYEAILTPDALELVAALHRAFEPRRQALLQARVERTKRLDAGERPDFLPETKAIREGDWKV
APLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFEDSNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYKLNDKIA
TLIVRPRGWHLDEKHVTVDGQRVSGGIFDFALFLFHNAKELIARGSGPYFYLPKMESHLEARLWNDIFVAAQEAIGIPRG
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLADRSKITMTVPFMRAYALLLLKTCHK
RNAPAIGGMSALIPIKNDPEANEKAMAGVRSDKQRDATDGYDGGWVAHPGLVPIAMEEFVKVLGDKPNQIGKQRDDVQVE
GKNLLDFQPEAPITEAGLRNNINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLEDGRKVTAELV
REFAKAELENVKRSVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI
>Mature_529_residues
STPITLPQGLAITGEIKPGYEAILTPDALELVAALHRAFEPRRQALLQARVERTKRLDAGERPDFLPETKAIREGDWKVA
PLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFEDSNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYKLNDKIAT
LIVRPRGWHLDEKHVTVDGQRVSGGIFDFALFLFHNAKELIARGSGPYFYLPKMESHLEARLWNDIFVAAQEAIGIPRGT
IRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLADRSKITMTVPFMRAYALLLLKTCHKR
NAPAIGGMSALIPIKNDPEANEKAMAGVRSDKQRDATDGYDGGWVAHPGLVPIAMEEFVKVLGDKPNQIGKQRDDVQVEG
KNLLDFQPEAPITEAGLRNNINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLEDGRKVTAELVR
EFAKAELENVKRSVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=536, Percent_Identity=49.4402985074627, Blast_Score=511, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17561814, Length=524, Percent_Identity=52.2900763358779, Blast_Score=534, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71982926, Length=420, Percent_Identity=54.047619047619, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6324212, Length=514, Percent_Identity=53.6964980544747, Blast_Score=546, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6322222, Length=514, Percent_Identity=51.556420233463, Blast_Score=516, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 59207; Mature: 59076

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPITLPQGLAITGEIKPGYEAILTPDALELVAALHRAFEPRRQALLQARVERTKRLDA
CCCCCCCCCCCEEEECCCCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
GERPDFLPETKAIREGDWKVAPLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFED
CCCCCCCCCCHHHHCCCCEEECCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHCCCC
SNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYKLNDKIATLIVRPRGWHLDEKHVTVDG
CCCCCCHHCCCCEEEHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCCCCCEEEECC
QRVSGGIFDFALFLFHNAKELIARGSGPYFYLPKMESHLEARLWNDIFVAAQEAIGIPRG
CCCCCHHHHHHHHHHHCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCC
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLADRSKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEE
TMTVPFMRAYALLLLKTCHKRNAPAIGGMSALIPIKNDPEANEKAMAGVRSDKQRDATDG
EEECHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCC
YDGGWVAHPGLVPIAMEEFVKVLGDKPNQIGKQRDDVQVEGKNLLDFQPEAPITEAGLRN
CCCCEEECCCCCHHHHHHHHHHHCCCCHHHCCCCCCEEECCCCCCCCCCCCCCHHHCCCC
NINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLEDGRKVTAELV
CCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHH
REFAKAELENVKRSVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHCC
>Mature Secondary Structure 
STPITLPQGLAITGEIKPGYEAILTPDALELVAALHRAFEPRRQALLQARVERTKRLDA
CCCCCCCCCCEEEECCCCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
GERPDFLPETKAIREGDWKVAPLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFED
CCCCCCCCCCHHHHCCCCEEECCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHCCCC
SNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYKLNDKIATLIVRPRGWHLDEKHVTVDG
CCCCCCHHCCCCEEEHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCCCCCEEEECC
QRVSGGIFDFALFLFHNAKELIARGSGPYFYLPKMESHLEARLWNDIFVAAQEAIGIPRG
CCCCCHHHHHHHHHHHCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCC
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLADRSKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEE
TMTVPFMRAYALLLLKTCHKRNAPAIGGMSALIPIKNDPEANEKAMAGVRSDKQRDATDG
EEECHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCC
YDGGWVAHPGLVPIAMEEFVKVLGDKPNQIGKQRDDVQVEGKNLLDFQPEAPITEAGLRN
CCCCEEECCCCCHHHHHHHHHHHCCCCHHHCCCCCCEEECCCCCCCCCCCCCCHHHCCCC
NINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLEDGRKVTAELV
CCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHH
REFAKAELENVKRSVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA