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The map label for this gene is gltX [H]

Identifier: 161524393

GI number: 161524393

Start: 1340587

End: 1341996

Strand: Direct

Name: gltX [H]

Synonym: Bmul_1220

Alternate gene names: 161524393

Gene position: 1340587-1341996 (Clockwise)

Preceding gene: 161524391

Following gene: 161524394

Centisome position: 38.87

GC content: 66.95

Gene sequence:

>1410_bases
ATGACCCGTCCTGTCCGTACCCGCTTCGCGCCGAGTCCCACCGGCTTCATCCACCTCGGCAACATCCGCTCCGCGCTTTA
TCCGTGGGCGTTCGCGCGCAAGATGAAAGGCACGTTCGTGCTGCGTATCGAGGATACCGACGTCGAGCGCTCGTCGAAGG
AGGCCGTCGACGCGATCCTCGAAGGGATGGAATGGCTCGGTCTCGATTTCGACGAAGGCCCGATCTACCAGATGCAGCGG
ATGGACCGCTATCGCGAGGTGCTCGCGCAGATGCTGGAGAAGGGGCTGGCCTACCCGTGCTACATGTCGGCCGAGGAACT
CGACGCGCTGCGCGAACGCCAGCGCGCGGCCGGTCTGAAGCCGCGCTACGACGGCACGTGGCGTCCTGAGCCCGGCAAGG
TGCTGCCGGAGCCGCCGCCGGGCGTGAAGCCGGTGCTGCGTTTCCGCAACCCGTTGACGGGCACCGTCGCGTGGGACGAC
GCCGTGAAGGGGCGCGTCGAGATCTCGAACGAGGAACTCGACGATCTCGTGATCGCGCGTCCGGACGGCACGCCGATCTA
CAACTTCTGCGTGGTTGTCGACGACATGGACATGGGCATCACGCACGTGATCCGCGGCGACGATCACGTGAACAACACGC
CGCGCCAGATCAACATCCTGCGCGCGCTCGGCGGCGAGCCGCCCGTGTATGCGCACCTGCCGACCGTGCTGAACGAGCAG
GGCGAGAAGATGAGCAAGCGTCACGGCGCGATGAGCGTGATGGCGTATCGCGACGCCGGTTATCTGCCGGAAGCGGTCGT
CAACTATCTCGCGCGTCTGGGCTGGTCGCACGGCGATGCGGAAATCTTCTCGCGCGAGCAGTTCGTCGAATGGTTCGATC
TCGAGCATCTCGGCAAGTCGCCCGCGCAGTACGACCACAACAAGCTGAACTGGCTGAACGCGCACTACATCAAGGAAGCC
GACAACGCGCGCCTGGCTGCGCTCGCGAAACCGTTCCTCGCGGCGCTGGCGATCGACGATGCGGCGCTCGCGGCCGGCCC
GGCGCTCGAGGCCGTGATCGCGCTGATGAAGGATCGCGCGACGACGGTCAAGGAAATCGCGGAAGGCGCGGCGATGTTCT
ACCGCGTGCCGGCGCCGGACGCCGATGCGCTCGCACAGCACGTGAGCGACGCCGTGCGGCCGGCACTGGCCGATCTCGCC
GCTGCGCTGAAGGCGGCCGACTGGACGAAGGAAGCGATCTCGGCCGCGCTGAAGGCGACGCTTGCAGCGCACAAGCTGAA
GATGCCGCAGCTCGCGATGCCGGTGCGCCTGCTCGTGGCGGGCACCACGCATACGCCGTCGATCGACGCGGTGCTCGCGC
TGTTCGATCGCGACGTCGTCGTGTCGCGCATCGAGGCGGCGCTCGCGTAA

Upstream 100 bases:

>100_bases
CTTTCCCGTTCCGGGCGTCGCCGGCACGCAGCCGCTCGCATGGCCGCCGCCGATCCCGCGTCGAATCATTCATGCGCCCC
AGGCGTTCGAGTTACCGTTT

Downstream 100 bases:

>100_bases
GCGCGCGGCGACGCGAGGTCGCGCAGCGTCTGCTGACGCGGCTTCGCAAAAAATTTCGCCCGATTCGCAAAAAGCTATTT
ACAACTGCAAAGTAGGTCCA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSKEAVDAILEGMEWLGLDFDEGPIYQMQR
MDRYREVLAQMLEKGLAYPCYMSAEELDALRERQRAAGLKPRYDGTWRPEPGKVLPEPPPGVKPVLRFRNPLTGTVAWDD
AVKGRVEISNEELDDLVIARPDGTPIYNFCVVVDDMDMGITHVIRGDDHVNNTPRQINILRALGGEPPVYAHLPTVLNEQ
GEKMSKRHGAMSVMAYRDAGYLPEAVVNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKSPAQYDHNKLNWLNAHYIKEA
DNARLAALAKPFLAALAIDDAALAAGPALEAVIALMKDRATTVKEIAEGAAMFYRVPAPDADALAQHVSDAVRPALADLA
AALKAADWTKEAISAALKATLAAHKLKMPQLAMPVRLLVAGTTHTPSIDAVLALFDRDVVVSRIEAALA

Sequences:

>Translated_469_residues
MTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSKEAVDAILEGMEWLGLDFDEGPIYQMQR
MDRYREVLAQMLEKGLAYPCYMSAEELDALRERQRAAGLKPRYDGTWRPEPGKVLPEPPPGVKPVLRFRNPLTGTVAWDD
AVKGRVEISNEELDDLVIARPDGTPIYNFCVVVDDMDMGITHVIRGDDHVNNTPRQINILRALGGEPPVYAHLPTVLNEQ
GEKMSKRHGAMSVMAYRDAGYLPEAVVNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKSPAQYDHNKLNWLNAHYIKEA
DNARLAALAKPFLAALAIDDAALAAGPALEAVIALMKDRATTVKEIAEGAAMFYRVPAPDADALAQHVSDAVRPALADLA
AALKAADWTKEAISAALKATLAAHKLKMPQLAMPVRLLVAGTTHTPSIDAVLALFDRDVVVSRIEAALA
>Mature_468_residues
TRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSKEAVDAILEGMEWLGLDFDEGPIYQMQRM
DRYREVLAQMLEKGLAYPCYMSAEELDALRERQRAAGLKPRYDGTWRPEPGKVLPEPPPGVKPVLRFRNPLTGTVAWDDA
VKGRVEISNEELDDLVIARPDGTPIYNFCVVVDDMDMGITHVIRGDDHVNNTPRQINILRALGGEPPVYAHLPTVLNEQG
EKMSKRHGAMSVMAYRDAGYLPEAVVNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKSPAQYDHNKLNWLNAHYIKEAD
NARLAALAKPFLAALAIDDAALAAGPALEAVIALMKDRATTVKEIAEGAAMFYRVPAPDADALAQHVSDAVRPALADLAA
ALKAADWTKEAISAALKATLAAHKLKMPQLAMPVRLLVAGTTHTPSIDAVLALFDRDVVVSRIEAALA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=330, Percent_Identity=36.3636363636364, Blast_Score=202, Evalue=5e-52,
Organism=Escherichia coli, GI1788743, Length=466, Percent_Identity=49.3562231759657, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI226510925, Length=245, Percent_Identity=37.5510204081633, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1786895, Length=107, Percent_Identity=30.8411214953271, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=471, Percent_Identity=28.4501061571125, Blast_Score=179, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6324540, Length=420, Percent_Identity=32.1428571428571, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321192, Length=233, Percent_Identity=26.6094420600858, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=123, Percent_Identity=32.520325203252, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=29.4715447154472, Blast_Score=218, Evalue=6e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 51802; Mature: 51671

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSKEAVDAIL
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
EGMEWLGLDFDEGPIYQMQRMDRYREVLAQMLEKGLAYPCYMSAEELDALRERQRAAGLK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
PRYDGTWRPEPGKVLPEPPPGVKPVLRFRNPLTGTVAWDDAVKGRVEISNEELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEECCCCCCEEEECCCCCCCEEEEC
PDGTPIYNFCVVVDDMDMGITHVIRGDDHVNNTPRQINILRALGGEPPVYAHLPTVLNEQ
CCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHH
GEKMSKRHGAMSVMAYRDAGYLPEAVVNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKS
HHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PAQYDHNKLNWLNAHYIKEADNARLAALAKPFLAALAIDDAALAAGPALEAVIALMKDRA
CCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHH
TTVKEIAEGAAMFYRVPAPDADALAQHVSDAVRPALADLAAALKAADWTKEAISAALKAT
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAHKLKMPQLAMPVRLLVAGTTHTPSIDAVLALFDRDVVVSRIEAALA
HHHHHCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSKEAVDAIL
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
EGMEWLGLDFDEGPIYQMQRMDRYREVLAQMLEKGLAYPCYMSAEELDALRERQRAAGLK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
PRYDGTWRPEPGKVLPEPPPGVKPVLRFRNPLTGTVAWDDAVKGRVEISNEELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEECCCCCCEEEECCCCCCCEEEEC
PDGTPIYNFCVVVDDMDMGITHVIRGDDHVNNTPRQINILRALGGEPPVYAHLPTVLNEQ
CCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHH
GEKMSKRHGAMSVMAYRDAGYLPEAVVNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKS
HHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PAQYDHNKLNWLNAHYIKEADNARLAALAKPFLAALAIDDAALAAGPALEAVIALMKDRA
CCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHH
TTVKEIAEGAAMFYRVPAPDADALAQHVSDAVRPALADLAAALKAADWTKEAISAALKAT
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAHKLKMPQLAMPVRLLVAGTTHTPSIDAVLALFDRDVVVSRIEAALA
HHHHHCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA