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The map label for this gene is tilS [H]

Identifier: 161524379

GI number: 161524379

Start: 1322456

End: 1323862

Strand: Direct

Name: tilS [H]

Synonym: Bmul_1206

Alternate gene names: 161524379

Gene position: 1322456-1323862 (Clockwise)

Preceding gene: 161524378

Following gene: 161524380

Centisome position: 38.35

GC content: 72.64

Gene sequence:

>1407_bases
GTGATCCCGCCGAACCAATACTCCGCCGACCGTGTCGTCCTCGATGCGGTCGGCGTTGCGCTTTCCGGTGTGCCGGACGG
CGCGCGGATCGCGATCGCGTATAGCGGCGGCCTCGATTCGTCGGTACTGCTCGACGCGGCCGTGCGCGTGGCCGGCGCGT
CGCGCTGCGTCGCACTGCACGTCCATCACGGCCTGAGCGCGAACGCCGACGCGTGGGCTGCGCATGCGCGCGAGCAGGCC
GCACGGCTCGGTGTCGCGTTCGAGGCGATGCATGTCGACGTGCCGCGCGATAGCGGCGTCGGCATCGAGGCGAGTGCGCG
CGAGCGTCGCTATGCGGCGCTCGACGCGATGTGCGCACGGCACGGCGCGGCTGCGCTCTGGCTCGCGCAGCATGCGGACG
ATCAGGCCGAAACCGTGCTGCTGCAACTGCTGCGCGGTGCAGGGATTGCCGGGCTCGCCGCGATGGCGCCGCGCTATCGG
CCCGACGGCGCGTCCGTCGAGCGTGTGCGGCCGCTGCTGCGATTGTTGCGTGCGCAACTGGAGCGTTATGCGGCGGAGCA
CGCGCTGTCGTGGATCGACGACGAATCGAACGCCGATACGCGCTATGCGCGCAACGCGCTGCGCATCGACGTACTGCCGG
CGCTGGCCGTGCATTTCCCCGGCTTTCGCGACGCACTCGGCCGCGCCGCGCAGCATGCGGCGGCGGCGCAGCGACTGCTC
GACGATCTGGCCGAGCTCGATTTCGCGGCGGCCGCGCGCGACGACGGACGTGCGTTGTCGCGCGATGCGCTGATCGCGTT
CGACGACGTGCGCGGCGCGAACCTGCTGCGCTACTGGATGCGCCGGCTCGGCTTGCCCGGCGCGTCGGCCGCGCGGCTGG
CCGACATGATGCGCCAGCTGCGTGACGCGCACGATGCGCATGCGCTGCGCGTCGATCATGCGGGGCAGTGCCTGCGCCTC
TATCGCGATACCGTTTACTGGGAAGCCGGTGATAGCGCGGAGCCGCGCGACGACGGCACCGGCACGCCGCACGCCGACGC
GTCGCTCGCGTGGGACGGCCAGGAGATCTGGCGTCTGCCCGGATGGCGCGGCACGTTCGTGTTCGCGCGCGCCGACGCCG
GCAGCGACGACGCGCTGCCCGAGACGCTGCTGCGCAGCGCGCCGCTGATCGCGCGCGCACGCGCGGGGGGCGAGCGGATG
CGCATGTGGCCGGACGGCCCGAGCCGGACGCTGAAGAACCTGTTTCAGGAGCGCGGCGTGCCCGCGTGGCAGCGCGACGT
GCCGCTGCTGTTCGCGGGCGACCGGCTGATCTACGTGCCGCGGCTCGGCGTGAACCGCGATGGCGTGCCGCAGGGCGGCC
ACGCATGGCGCCGCATCGAATGGCGGCCCGACCTGCTGATCGCGTGA

Upstream 100 bases:

>100_bases
TTCGAACGCCTGATGGCGTACGGCAAGTTCAAGGAAACCACGCCGGGCGCATGACCGGCTGCGTACCTGCTGCGCGCGTC
ACGATGCGCGCATCCGCCTC

Downstream 100 bases:

>100_bases
CGGCGACCGGTCGACAACGGCGCGCCGCGCGGCGCTGTCGGCCGCACCGGTAAAGGGCGCCGGAACACGCGCGGACGCGG
CCGGCGGCTTGTCAAGCGGG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MIPPNQYSADRVVLDAVGVALSGVPDGARIAIAYSGGLDSSVLLDAAVRVAGASRCVALHVHHGLSANADAWAAHAREQA
ARLGVAFEAMHVDVPRDSGVGIEASARERRYAALDAMCARHGAAALWLAQHADDQAETVLLQLLRGAGIAGLAAMAPRYR
PDGASVERVRPLLRLLRAQLERYAAEHALSWIDDESNADTRYARNALRIDVLPALAVHFPGFRDALGRAAQHAAAAQRLL
DDLAELDFAAAARDDGRALSRDALIAFDDVRGANLLRYWMRRLGLPGASAARLADMMRQLRDAHDAHALRVDHAGQCLRL
YRDTVYWEAGDSAEPRDDGTGTPHADASLAWDGQEIWRLPGWRGTFVFARADAGSDDALPETLLRSAPLIARARAGGERM
RMWPDGPSRTLKNLFQERGVPAWQRDVPLLFAGDRLIYVPRLGVNRDGVPQGGHAWRRIEWRPDLLIA

Sequences:

>Translated_468_residues
MIPPNQYSADRVVLDAVGVALSGVPDGARIAIAYSGGLDSSVLLDAAVRVAGASRCVALHVHHGLSANADAWAAHAREQA
ARLGVAFEAMHVDVPRDSGVGIEASARERRYAALDAMCARHGAAALWLAQHADDQAETVLLQLLRGAGIAGLAAMAPRYR
PDGASVERVRPLLRLLRAQLERYAAEHALSWIDDESNADTRYARNALRIDVLPALAVHFPGFRDALGRAAQHAAAAQRLL
DDLAELDFAAAARDDGRALSRDALIAFDDVRGANLLRYWMRRLGLPGASAARLADMMRQLRDAHDAHALRVDHAGQCLRL
YRDTVYWEAGDSAEPRDDGTGTPHADASLAWDGQEIWRLPGWRGTFVFARADAGSDDALPETLLRSAPLIARARAGGERM
RMWPDGPSRTLKNLFQERGVPAWQRDVPLLFAGDRLIYVPRLGVNRDGVPQGGHAWRRIEWRPDLLIA
>Mature_468_residues
MIPPNQYSADRVVLDAVGVALSGVPDGARIAIAYSGGLDSSVLLDAAVRVAGASRCVALHVHHGLSANADAWAAHAREQA
ARLGVAFEAMHVDVPRDSGVGIEASARERRYAALDAMCARHGAAALWLAQHADDQAETVLLQLLRGAGIAGLAAMAPRYR
PDGASVERVRPLLRLLRAQLERYAAEHALSWIDDESNADTRYARNALRIDVLPALAVHFPGFRDALGRAAQHAAAAQRLL
DDLAELDFAAAARDDGRALSRDALIAFDDVRGANLLRYWMRRLGLPGASAARLADMMRQLRDAHDAHALRVDHAGQCLRL
YRDTVYWEAGDSAEPRDDGTGTPHADASLAWDGQEIWRLPGWRGTFVFARADAGSDDALPETLLRSAPLIARARAGGERM
RMWPDGPSRTLKNLFQERGVPAWQRDVPLLFAGDRLIYVPRLGVNRDGVPQGGHAWRRIEWRPDLLIA

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=439, Percent_Identity=35.0797266514806, Blast_Score=171, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 50932; Mature: 50932

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPPNQYSADRVVLDAVGVALSGVPDGARIAIAYSGGLDSSVLLDAAVRVAGASRCVALH
CCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
VHHGLSANADAWAAHAREQAARLGVAFEAMHVDVPRDSGVGIEASARERRYAALDAMCAR
EECCCCCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
HGAAALWLAQHADDQAETVLLQLLRGAGIAGLAAMAPRYRPDGASVERVRPLLRLLRAQL
CCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
ERYAAEHALSWIDDESNADTRYARNALRIDVLPALAVHFPGFRDALGRAAQHAAAAQRLL
HHHHHHHHHHHCCCCCCCCHHHHHHHHEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DDLAELDFAAAARDDGRALSRDALIAFDDVRGANLLRYWMRRLGLPGASAARLADMMRQL
HHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RDAHDAHALRVDHAGQCLRLYRDTVYWEAGDSAEPRDDGTGTPHADASLAWDGQEIWRLP
HHHHHHHEEEECCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCEEEECCHHHHCCC
GWRGTFVFARADAGSDDALPETLLRSAPLIARARAGGERMRMWPDGPSRTLKNLFQERGV
CCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCC
PAWQRDVPLLFAGDRLIYVPRLGVNRDGVPQGGHAWRRIEWRPDLLIA
CCHHCCCCEEEECCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MIPPNQYSADRVVLDAVGVALSGVPDGARIAIAYSGGLDSSVLLDAAVRVAGASRCVALH
CCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
VHHGLSANADAWAAHAREQAARLGVAFEAMHVDVPRDSGVGIEASARERRYAALDAMCAR
EECCCCCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
HGAAALWLAQHADDQAETVLLQLLRGAGIAGLAAMAPRYRPDGASVERVRPLLRLLRAQL
CCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
ERYAAEHALSWIDDESNADTRYARNALRIDVLPALAVHFPGFRDALGRAAQHAAAAQRLL
HHHHHHHHHHHCCCCCCCCHHHHHHHHEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DDLAELDFAAAARDDGRALSRDALIAFDDVRGANLLRYWMRRLGLPGASAARLADMMRQL
HHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RDAHDAHALRVDHAGQCLRLYRDTVYWEAGDSAEPRDDGTGTPHADASLAWDGQEIWRLP
HHHHHHHEEEECCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCEEEECCHHHHCCC
GWRGTFVFARADAGSDDALPETLLRSAPLIARARAGGERMRMWPDGPSRTLKNLFQERGV
CCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCC
PAWQRDVPLLFAGDRLIYVPRLGVNRDGVPQGGHAWRRIEWRPDLLIA
CCHHCCCCEEEECCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA