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The map label for this gene is nuoF2 [H]

Identifier: 161524209

GI number: 161524209

Start: 1128098

End: 1129408

Strand: Direct

Name: nuoF2 [H]

Synonym: Bmul_1033

Alternate gene names: 161524209

Gene position: 1128098-1129408 (Clockwise)

Preceding gene: 161524208

Following gene: 161524210

Centisome position: 32.71

GC content: 65.14

Gene sequence:

>1311_bases
ATGACGTCCCTCCACGACCGTCACATCAAACCGCTGATCCTCGCTGGTCTGAACGGCGAGAACTGGCATCTCGAAGACTA
CGTTGCGCGCGGCGGCTACAAGCAGCTGCGCCGCATTCTCGAAGAAAAGATTCCGCCCGAGCAGGTGATCGCCGACGTCA
AGGCGTCGGGCCTGCGCGGCCGCGGCGGTGCGGGCTTCCCGACCGGCCTGAAGTGGAGCTTCATGCCGCGTCAGTTCCCG
GGGCAGAAGTACCTCGTCTGCAACTCGGACGAAGGCGAGCCGGGCACGTTCAAGGATCGCGACATCCTGCGCTGGAATCC
GCACGCGCTGATCGAAGGGATGGCCATCGGCGCCTACGCGATGGGCATCACGGTCGGCTACAACTACATCCACGGCGAGA
TCTTCGAAGTGTATCGACGCTTCGAGGCGGCGCTGGAAGAGGCACGCGCGGCCGGTTTTCTCGGCGACAACATCATGGGC
TCGGAATTCTCGTTCCAGCTCCATGCGCATCACGGCTACGGCGCGTACATCTGCGGCGAGGAAACGGCGCTGCTCGAGTC
GCTCGAAGGCAAGAAGGGCCAGCCGCGCTTCAAGCCGCCGTTCCCGGCGAGCTTCGGCGTGTACGGCAAGCCCACGACGA
TCAACAACACCGAGACGTTCGCCGCGGTGCCGTTCCTGCTCGCCGTCGGCCCGCAGAATTACCTCGAGCTCGGCAAGCCG
AACAACGGCGGCACGAAGATCTTCTCGGTGTCGGGCGACGTCGAGCGTCCGGGCAACTATGAAGTGCCGCTCGGCACGCC
GTTCGCGACGCTGATGGAGCTCGCCGGCGGGATGCGCGGCGGCAAGAAGATCAAGGCCGTGATTCCGGGCGGCTCGTCCG
CACCGGTGGTGCCGGGCGACATCATGATGCAGACCGACATGGACTACGACTCGATCGCGAAGGTCGGCTCGATGCTCGGT
TCGGGTGCGGTGATCGTGATGGACGAGACGCGCTGCATGGTGCGCTCGCTGCTGCGCCTGTCGTACTTCTACTACGAGGA
ATCGTGCGGTCAGTGCACGCCGTGCCGCGAAGGTACGGGCTGGCTGTATCGCGTCGTCAACCGCATCGAGCACGGCGAAG
GCCGCCAGGAAGACCTGGATCTGCTCAATTCGGTCGCCGAGAACATCATGGGGCGCACGATCTGCGCGCTGGGCGATGCG
GCTGCGATGCCGGTGCGCGGGATGCTCAAGCACTACTGGGACGAATTCGCGTACCACGTCGAGCACAAGCGCTGCATGGT
CGGCGGTCACGCGCACGCGGCGGCGGCCTGA

Upstream 100 bases:

>100_bases
GCGCGTGCGGCGACGCCCCGGTGCTGCTGGTGAACAATCACAAAATGTGCAGCTTCATGAGCCGCGAGAAGATCGACCAG
CTGCTTGAGGAGCTCTCGAA

Downstream 100 bases:

>100_bases
CGCGAAACCGGTTGCGGAATTTGAGCGCACGGTCGGGCGAACGCGCGAACGGGCGGAAATAGGTTAAGGACCATTCACCA
TCATGGTTGAACTTGAAATA

Product: NADH-quinone oxidoreductase subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTSLHDRHIKPLILAGLNGENWHLEDYVARGGYKQLRRILEEKIPPEQVIADVKASGLRGRGGAGFPTGLKWSFMPRQFP
GQKYLVCNSDEGEPGTFKDRDILRWNPHALIEGMAIGAYAMGITVGYNYIHGEIFEVYRRFEAALEEARAAGFLGDNIMG
SEFSFQLHAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGVYGKPTTINNTETFAAVPFLLAVGPQNYLELGKP
NNGGTKIFSVSGDVERPGNYEVPLGTPFATLMELAGGMRGGKKIKAVIPGGSSAPVVPGDIMMQTDMDYDSIAKVGSMLG
SGAVIVMDETRCMVRSLLRLSYFYYEESCGQCTPCREGTGWLYRVVNRIEHGEGRQEDLDLLNSVAENIMGRTICALGDA
AAMPVRGMLKHYWDEFAYHVEHKRCMVGGHAHAAAA

Sequences:

>Translated_436_residues
MTSLHDRHIKPLILAGLNGENWHLEDYVARGGYKQLRRILEEKIPPEQVIADVKASGLRGRGGAGFPTGLKWSFMPRQFP
GQKYLVCNSDEGEPGTFKDRDILRWNPHALIEGMAIGAYAMGITVGYNYIHGEIFEVYRRFEAALEEARAAGFLGDNIMG
SEFSFQLHAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGVYGKPTTINNTETFAAVPFLLAVGPQNYLELGKP
NNGGTKIFSVSGDVERPGNYEVPLGTPFATLMELAGGMRGGKKIKAVIPGGSSAPVVPGDIMMQTDMDYDSIAKVGSMLG
SGAVIVMDETRCMVRSLLRLSYFYYEESCGQCTPCREGTGWLYRVVNRIEHGEGRQEDLDLLNSVAENIMGRTICALGDA
AAMPVRGMLKHYWDEFAYHVEHKRCMVGGHAHAAAA
>Mature_435_residues
TSLHDRHIKPLILAGLNGENWHLEDYVARGGYKQLRRILEEKIPPEQVIADVKASGLRGRGGAGFPTGLKWSFMPRQFPG
QKYLVCNSDEGEPGTFKDRDILRWNPHALIEGMAIGAYAMGITVGYNYIHGEIFEVYRRFEAALEEARAAGFLGDNIMGS
EFSFQLHAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGVYGKPTTINNTETFAAVPFLLAVGPQNYLELGKPN
NGGTKIFSVSGDVERPGNYEVPLGTPFATLMELAGGMRGGKKIKAVIPGGSSAPVVPGDIMMQTDMDYDSIAKVGSMLGS
GAVIVMDETRCMVRSLLRLSYFYYEESCGQCTPCREGTGWLYRVVNRIEHGEGRQEDLDLLNSVAENIMGRTICALGDAA
AMPVRGMLKHYWDEFAYHVEHKRCMVGGHAHAAAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=425, Percent_Identity=45.4117647058823, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI260656005, Length=425, Percent_Identity=45.4117647058823, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1788620, Length=402, Percent_Identity=42.5373134328358, Blast_Score=331, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17531727, Length=419, Percent_Identity=44.8687350835322, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI19920794, Length=424, Percent_Identity=43.6320754716981, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45550452, Length=415, Percent_Identity=43.855421686747, Blast_Score=351, Evalue=5e-97,
Organism=Drosophila melanogaster, GI24653819, Length=398, Percent_Identity=36.9346733668342, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24653817, Length=398, Percent_Identity=33.1658291457286, Blast_Score=214, Evalue=9e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 47809; Mature: 47678

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLHDRHIKPLILAGLNGENWHLEDYVARGGYKQLRRILEEKIPPEQVIADVKASGLRG
CCCCCCCCCCEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCC
RGGAGFPTGLKWSFMPRQFPGQKYLVCNSDEGEPGTFKDRDILRWNPHALIEGMAIGAYA
CCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCHHHHHHHHHHHEE
MGITVGYNYIHGEIFEVYRRFEAALEEARAAGFLGDNIMGSEFSFQLHAHHGYGAYICGE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEEECC
ETALLESLEGKKGQPRFKPPFPASFGVYGKPTTINNTETFAAVPFLLAVGPQNYLELGKP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCC
NNGGTKIFSVSGDVERPGNYEVPLGTPFATLMELAGGMRGGKKIKAVIPGGSSAPVVPGD
CCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
IMMQTDMDYDSIAKVGSMLGSGAVIVMDETRCMVRSLLRLSYFYYEESCGQCTPCREGTG
EEEECCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
WLYRVVNRIEHGEGRQEDLDLLNSVAENIMGRTICALGDAAAMPVRGMLKHYWDEFAYHV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHH
EHKRCMVGGHAHAAAA
HHCEEEECCCHHHCCC
>Mature Secondary Structure 
TSLHDRHIKPLILAGLNGENWHLEDYVARGGYKQLRRILEEKIPPEQVIADVKASGLRG
CCCCCCCCCEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCC
RGGAGFPTGLKWSFMPRQFPGQKYLVCNSDEGEPGTFKDRDILRWNPHALIEGMAIGAYA
CCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCHHHHHHHHHHHEE
MGITVGYNYIHGEIFEVYRRFEAALEEARAAGFLGDNIMGSEFSFQLHAHHGYGAYICGE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEEECC
ETALLESLEGKKGQPRFKPPFPASFGVYGKPTTINNTETFAAVPFLLAVGPQNYLELGKP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCC
NNGGTKIFSVSGDVERPGNYEVPLGTPFATLMELAGGMRGGKKIKAVIPGGSSAPVVPGD
CCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
IMMQTDMDYDSIAKVGSMLGSGAVIVMDETRCMVRSLLRLSYFYYEESCGQCTPCREGTG
EEEECCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
WLYRVVNRIEHGEGRQEDLDLLNSVAENIMGRTICALGDAAAMPVRGMLKHYWDEFAYHV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHH
EHKRCMVGGHAHAAAA
HHCEEEECCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]