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The map label for this gene is ilvI [H]

Identifier: 161524189

GI number: 161524189

Start: 1107366

End: 1109129

Strand: Direct

Name: ilvI [H]

Synonym: Bmul_1013

Alternate gene names: 161524189

Gene position: 1107366-1109129 (Clockwise)

Preceding gene: 161524177

Following gene: 161524190

Centisome position: 32.11

GC content: 63.1

Gene sequence:

>1764_bases
ATGAACATGCCCAGCGCGGAATTCTCCACGTCGGACCCCCTTTCCCCTCCCGATAGCGACTCCATCGGCGCCACCGTGCT
CATGAAGGCACTGGCCGACGAAAACGTCGAATTCATCTGGGGCTATCCCGGCGGCTCGGTTCTCTATATCTACGACGAGC
TTTACAAGCAGGACAAGATTCAGCACGTGCTGGTGCGCCACGAACAGGCGGCCGTGCACGCAGCCGATGCGTATGCGCGC
TCCACCGGCAAGGTCGGCGTGTGCCTCGTGACGTCGGGCCCCGGCGTGACGAACGCGGTCACCGGCATCGCGACGGCCTA
CATGGATTCGATCCCGATGGTCGTCATCAGCGGCCAGGTGCCCACCGCCGCAATCGGCCAGGACGCGTTCCAGGAATGCG
ATACGGTCGGCATCACGCGTCCGTGCGTGAAGCACAACTTCCTCGTGAAGGACGTGCGCGACCTCGCGGAGACCGTCAAG
AAGGCGTTCTACATCGCACGCACCGGCCGTCCGGGCCCGGTGCTGATCGACATCCCGAAGGACATCTCGAAGACGCCGTG
CCAGTACGAGCCCGTCAAGAGCGTGTCGCTGCGTTCGTACAACCCCGTCACGAAGGGCCACTCGGGCCAGATCCGCAAGG
CCGTGTCGCTGCTGCTGTCGGCCAAGCGTCCGTACATCTATACGGGCGGCGGCATCATCCTCGCCGATGCGTCGCGCGAG
CTGAACCAGTTCGCGGATCTGCTCGGCTATCCGGTCACCAACACGCTGATGGGCCTCGGCGGCTATCGCGCCTCCGACAA
GAAATTCCTCGGCATGCTCGGCATGCACGGCACCTACGAAGCCAACATGGCGATGCAGCACTGCGACGTGCTGATCGCGA
TCGGCGCACGCTTCGACGATCGCGTGATCGGCGATCCGGCGCACTTCGCATCGCGTCCGCGCAAGATCATCCACATCGAC
ATCGACCCGTCGTCGATCTCGAAGCGCGTGAAGGTCGACATCCCGATCGTCGGCGACGTGAAGGAAGTGCTGAAGGAACT
GATCGAGCAGCTGCAGACGGCCGAGCACGGCCCCGACACCGAAGCGCTCGCGCAATGGTGGAAGGACATCGAGAGCTGGC
GCGCGAAGGACTGCCTGAAGTACGACCGCGAGAGCGAGATCATCAAGCCGCAGTACGTGGTCGAGAAGGCGTGGGAACTG
ACGGACGGCAACGCGTTCGTGTGCTCCGACGTCGGCCAGCACCAGATGTGGGCTGCGCAGTTCTACCGTTTCAACAAGCC
GCGCCGCTGGATCAACTCCGGCGGGCTCGGCACGATGGGCTTCGGCCTGCCGGCGGCGATGGGCGTCAAGATGGCGCACC
CGGACGACGACGTGCTGTGCATCACGGGCGAAGGCTCGATCCAGATGTGCATCCAGGAGTTGTCGACCTGCCTGCAGTAC
GACACGCCCGTGAAGATCATTTCGCTGAACAACCGCTACCTCGGCATGGTTCGCCAGTGGCAGCAGATCGAATACAGCAA
GCGCTATTCGCATTCGTACATGGATGCGCTGCCCGATTTCGTGAAGCTCGCCGAAGCGTACGGCCATGTCGGCATGCGGA
TCGAAAAGACTTCGGATGTGGAGCCGGCGCTGAAGGAAGCGCTGCGCCTGAAGGACCGCACCGTGTTTCTCGACTTCCAG
ACCGATCCGACCGAAAACGTCTGGCCGATGGTCCAGGCCGGCAAGGGCATCACCGAGATGCTGCTCGGATCGGAAGATCT
GTAA

Upstream 100 bases:

>100_bases
ATGAAGCCCGCCCTTCAACTGGACGCGCCGCTTGAACCCGAGCCACAAGCCCGGCAGAGAGCACCCGATCAATTTTTTGC
CGAAAATTCGAAAGGTCAAA

Downstream 100 bases:

>100_bases
CGGCGCGACGCGCGCAGCGCGCGGGGCGGCCGCCTTCACGAAGGGCGGTGCGGCACGCGGCGGCGCGCGGCGCGAGTGAA
TCGACACGGACACATTCTGG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MNMPSAEFSTSDPLSPPDSDSIGATVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKIQHVLVRHEQAAVHAADAYAR
STGKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAETVK
KAFYIARTGRPGPVLIDIPKDISKTPCQYEPVKSVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTEALAQWWKDIESWRAKDCLKYDRESEIIKPQYVVEKAWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCLQY
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIEKTSDVEPALKEALRLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLLGSEDL

Sequences:

>Translated_587_residues
MNMPSAEFSTSDPLSPPDSDSIGATVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKIQHVLVRHEQAAVHAADAYAR
STGKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAETVK
KAFYIARTGRPGPVLIDIPKDISKTPCQYEPVKSVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTEALAQWWKDIESWRAKDCLKYDRESEIIKPQYVVEKAWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCLQY
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIEKTSDVEPALKEALRLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLLGSEDL
>Mature_587_residues
MNMPSAEFSTSDPLSPPDSDSIGATVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKIQHVLVRHEQAAVHAADAYAR
STGKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAETVK
KAFYIARTGRPGPVLIDIPKDISKTPCQYEPVKSVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTEALAQWWKDIESWRAKDCLKYDRESEIIKPQYVVEKAWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCLQY
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIEKTSDVEPALKEALRLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLLGSEDL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=583, Percent_Identity=25.0428816466552, Blast_Score=173, Evalue=4e-43,
Organism=Homo sapiens, GI21361361, Length=568, Percent_Identity=26.9366197183099, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI87081685, Length=571, Percent_Identity=58.1436077057793, Blast_Score=685, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=40.1785714285714, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=33.8951310861423, Blast_Score=311, Evalue=7e-86,
Organism=Escherichia coli, GI1787096, Length=566, Percent_Identity=28.2685512367491, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1788716, Length=554, Percent_Identity=24.0072202166065, Blast_Score=149, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=550, Percent_Identity=26.5454545454545, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=550, Percent_Identity=26.5454545454545, Blast_Score=163, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=515, Percent_Identity=27.7669902912621, Blast_Score=144, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=592, Percent_Identity=42.3986486486487, Blast_Score=458, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6320816, Length=475, Percent_Identity=24.4210526315789, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=518, Percent_Identity=20.4633204633205, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=498, Percent_Identity=21.8875502008032, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922626, Length=565, Percent_Identity=26.0176991150442, Blast_Score=182, Evalue=8e-46,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64982; Mature: 64982

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMPSAEFSTSDPLSPPDSDSIGATVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHH
QHVLVRHEQAAVHAADAYARSTGKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQV
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAETVKKAFYIARTGRPGPVLIDIPK
CCHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DISKTPCQYEPVKSVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIILADASRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHH
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDD
HHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCC
RVIGDPAHFASRPRKIIHIDIDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDT
CCCCCCHHHHCCCCEEEEEEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCH
EALAQWWKDIESWRAKDCLKYDRESEIIKPQYVVEKAWELTDGNAFVCSDVGQHQMWAAQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
FYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCLQY
HHHCCCCHHHHCCCCCCCCCCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHHHHHHHCC
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIEKTSDV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALKEALRLKDRTVFLDFQTDPTENVWPMVQAGKGITEMLLGSEDL
CHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC
>Mature Secondary Structure
MNMPSAEFSTSDPLSPPDSDSIGATVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHH
QHVLVRHEQAAVHAADAYARSTGKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQV
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAETVKKAFYIARTGRPGPVLIDIPK
CCHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DISKTPCQYEPVKSVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIILADASRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHH
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDD
HHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCC
RVIGDPAHFASRPRKIIHIDIDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDT
CCCCCCHHHHCCCCEEEEEEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCH
EALAQWWKDIESWRAKDCLKYDRESEIIKPQYVVEKAWELTDGNAFVCSDVGQHQMWAAQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
FYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCLQY
HHHCCCCHHHHCCCCCCCCCCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHHHHHHHCC
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIEKTSDV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALKEALRLKDRTVFLDFQTDPTENVWPMVQAGKGITEMLLGSEDL
CHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]