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The map label for this gene is rnfB [H]

Identifier: 161524127

GI number: 161524127

Start: 1037243

End: 1038205

Strand: Reverse

Name: rnfB [H]

Synonym: Bmul_0951

Alternate gene names: 161524127

Gene position: 1038205-1037243 (Counterclockwise)

Preceding gene: 161524129

Following gene: 161524126

Centisome position: 30.11

GC content: 71.55

Gene sequence:

>963_bases
ATGCGGGAAATCGGCGATAATCGGGGTTTGGCAAACCTTTCCGGCCGCGTCCGACGCGGCCGAGAGCCATTCGGCGCGAT
TGCGCCCGTTGTCACCGTGACCGATTCCAAGACACTCGCGGATCGCATCGAAGATCTGCTTCCCCAGACGCAATGCACGA
AGTGCGGCTATAACGGCTGCCGCCCGTACGCCGAGGCGATCGCCGCCGGCGACGCGAACTACAACCAGTGCCCGCCCGGC
GGCGCCGAAGGCATCGCGCGCCTCGCGAACCTGCTCGGCAAGCCGGTGATTCCGCTGAATCCCGTCAACGGCACCGAGCA
CCCGCGTGCGGTCGCGTTCATCGACGAAAACCTGTGCATCGGCTGCACGCTCTGCATGCAGGCGTGCCCGGTCGATGCGA
TCGTCGGTGCGCCGAAGCAGATGCACACGATCGTCGCGTCGCTCTGCACCGGCTGCGACCTCTGCGTGCCGCCGTGCCCG
GTCGACTGTATCGCGATGGTGCCCGTCACGGGCGAGCGCACCGGCTGGGACGCCTGGACGCAGGAGCAGGCCGACGCCGC
GCGCGAGCGGCACGATCGCCGGCTCGCGCGCCAGCGCCGCGAGCGCGACGCGGCCGAAGCGCGCGCCGCCGCACGCCGCG
CGGCGAGCGCCGCCGCGACGCAGCCGGCCGCAGCGCCGGCCGCCGCACCGCCCGCCGCCGACGATGCCGAAGCGAAAAAA
CGCGCGATCATCGCCGCCGCGCTCGAACGCGCGCGCAAGAAGAAGGAAGAACTCGCCGCACAGGGTGCGGCTCCGAAGAA
CACCGAAGGCGTGAGCGCGGCCGTCCAGGCGCAGATCGACGCAGCCGAGGCGCGCCGCAAGCGGCTCGCCGAACAGCAGG
CCGCGCGCGATGCGCAGGCCGACGACGCCGATCGAGACGATACCGACGGCCCGTCCGCGCCGCCCGACGACCAAGCGCCA
TGA

Upstream 100 bases:

>100_bases
CGCCGGCCGCCTTGGAACGGGCGTACCGCGTACAGTCGCCCTCCTGCTGCGCTCCTGCAAAAAAGCGGTTCGATTATGCG
CACAGAAGGCGTCCAGCGCA

Downstream 100 bases:

>100_bases
ACGCCAGCAAACGACGCGCGATCTACGAAACGCTGCAAAGCCTCAACCCGCATCCGACCACCGAACTCGAATACTCGACG
CCGTTCGAGCTGTTGATCGC

Product: ferredoxin

Products: NA

Alternate protein names: Nitrogen fixation protein rnfB [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MREIGDNRGLANLSGRVRRGREPFGAIAPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGCRPYAEAIAAGDANYNQCPPG
GAEGIARLANLLGKPVIPLNPVNGTEHPRAVAFIDENLCIGCTLCMQACPVDAIVGAPKQMHTIVASLCTGCDLCVPPCP
VDCIAMVPVTGERTGWDAWTQEQADAARERHDRRLARQRRERDAAEARAAARRAASAAATQPAAAPAAAPPAADDAEAKK
RAIIAAALERARKKKEELAAQGAAPKNTEGVSAAVQAQIDAAEARRKRLAEQQAARDAQADDADRDDTDGPSAPPDDQAP

Sequences:

>Translated_320_residues
MREIGDNRGLANLSGRVRRGREPFGAIAPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGCRPYAEAIAAGDANYNQCPPG
GAEGIARLANLLGKPVIPLNPVNGTEHPRAVAFIDENLCIGCTLCMQACPVDAIVGAPKQMHTIVASLCTGCDLCVPPCP
VDCIAMVPVTGERTGWDAWTQEQADAARERHDRRLARQRRERDAAEARAAARRAASAAATQPAAAPAAAPPAADDAEAKK
RAIIAAALERARKKKEELAAQGAAPKNTEGVSAAVQAQIDAAEARRKRLAEQQAARDAQADDADRDDTDGPSAPPDDQAP
>Mature_320_residues
MREIGDNRGLANLSGRVRRGREPFGAIAPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGCRPYAEAIAAGDANYNQCPPG
GAEGIARLANLLGKPVIPLNPVNGTEHPRAVAFIDENLCIGCTLCMQACPVDAIVGAPKQMHTIVASLCTGCDLCVPPCP
VDCIAMVPVTGERTGWDAWTQEQADAARERHDRRLARQRRERDAAEARAAARRAASAAATQPAAAPAAAPPAADDAEAKK
RAIIAAALERARKKKEELAAQGAAPKNTEGVSAAVQAQIDAAEARRKRLAEQQAARDAQADDADRDDTDGPSAPPDDQAP

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787915, Length=174, Percent_Identity=46.551724137931, Blast_Score=147, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4; PF04060 FeS [H]

EC number: NA

Molecular weight: Translated: 33661; Mature: 33661

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00198 4FE4S_FERREDOXIN ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREIGDNRGLANLSGRVRRGREPFGAIAPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGC
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCC
RPYAEAIAAGDANYNQCPPGGAEGIARLANLLGKPVIPLNPVNGTEHPRAVAFIDENLCI
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCHH
GCTLCMQACPVDAIVGAPKQMHTIVASLCTGCDLCVPPCPVDCIAMVPVTGERTGWDAWT
HHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCHHH
QEQADAARERHDRRLARQRRERDAAEARAAARRAASAAATQPAAAPAAAPPAADDAEAKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
RAIIAAALERARKKKEELAAQGAAPKNTEGVSAAVQAQIDAAEARRKRLAEQQAARDAQA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DDADRDDTDGPSAPPDDQAP
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MREIGDNRGLANLSGRVRRGREPFGAIAPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGC
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCC
RPYAEAIAAGDANYNQCPPGGAEGIARLANLLGKPVIPLNPVNGTEHPRAVAFIDENLCI
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCHH
GCTLCMQACPVDAIVGAPKQMHTIVASLCTGCDLCVPPCPVDCIAMVPVTGERTGWDAWT
HHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCHHH
QEQADAARERHDRRLARQRRERDAAEARAAARRAASAAATQPAAAPAAAPPAADDAEAKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
RAIIAAALERARKKKEELAAQGAAPKNTEGVSAAVQAQIDAAEARRKRLAEQQAARDAQA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DDADRDDTDGPSAPPDDQAP
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA