Click here to switch to the map view.

The map label for this gene is pcm [C]

Identifier: 161524085

GI number: 161524085

Start: 990526

End: 992523

Strand: Reverse

Name: pcm [C]

Synonym: Bmul_0906

Alternate gene names: 161524085

Gene position: 992523-990526 (Counterclockwise)

Preceding gene: 161524087

Following gene: 161524083

Centisome position: 28.78

GC content: 69.52

Gene sequence:

>1998_bases
ATGGTGGAACGGCAAATCGCAGCGCGCGGCGTACGCGATCCGCACGTACTCGACGCGATGCGCACCGTCGCTCGCCATCG
GTTCGTTCCCGCGGAACTCGCGGAATTCGCATACAGCGACACGCCGCTTCCGCTTGCCGACGACCAGACGATCTCGCAAC
CGATCGTCGTCGCGCGCATGCTGGAAGCCGCGACGCTCTCGCCGACCGATCGCTTGCTCGATGTCGGCACGGGCTCCGGA
TATGCGGCGGCGGTCGCATCGCGCATCGTCGCGCACGTCGACAGCATCGAGCGGCATGCGTCGCTCGCCGCATCCGCGCA
GCGCACGCTCGCGGAGAACGGCTTCGGCAACGTCGATGTCCATCACGCGGACGGCACGCTCGGCTGGCCCGCGCACGCCC
CGTTCGATGCGATCGTCGCGGCCGCAGGCGGGCCCGACGTGCCGCGCGCATGGCGCGAGCAACTCGCGATCGGCGGGCGC
ATCGTGATGCCGATCGGCGACGAGCGCGACGCGCAGCGGCTCGTGCGGATCACGCGCACCGGCGCGACGCAGTTCGAACA
CGAAGATCTCGGCGCGGTGCGGTTCGTGCCGCTGATCGGCGAACAGGGCTGGCCCGAGCACGATCCCGCACGCGACGGCG
ACACCGCGCGAGCCACGCGCGACATAGAGCACGACGCGCACGCCCCATACGTCGCAGCGCCGCCGACACCGCTCGCGCGC
ACGATCGCACGCGCGGCGCGGCCGCTGCCCGAGCCGGAGGATCCGACGTTCGCCGATGCGTTCGATCATCTCGCCGACAA
GCGCATCGTGCTGCTCGGCGAATGCAGTCACGGCACGTCGGAGTTCTATCGGGCGCGCGCGGCGATCACGCGGCGGCTCG
TCGAGCGGCACGGCTTCAACATCGTCGCGGTGGAAGCGGACTGGCCGGACGCGGCGATCGTGCATCGCTACGTGATGCTC
GACGAACGGCGCGACGCCGATCCGCCGTTCCAGCGTTTCCCGACCTGGATGTGGCGCAACGAGGAATTCGCCGCGTTCGT
CGAATGGCTGCGCGCGCACAACGGCCGCACGCGGCGCGAGGCGCGCTGCGGCTTTCACGGTCTGGACATGTACAGCCTCT
CCGCGTCGGTCGAGGCCGTGCTCGACTATCTCGATCGCTCGGACCCGAACGCGGCGCGCATCGCGCGCGAGCGCTACGGC
TGCCTGACGCCGTGGCAGAAGGATCCGCTCGTGTACGGGCGCGCGGCGATGTCGTCCGGATTCGAGAACTGCGAGGACGC
CGTGATCGCGCAGCTGCAGGACATGCTGCGCGGACGCATCGCGCGCGCAGGCGACGACGACCTGCATTTCGACGCGACGC
AGAACGCGCGGCTCGTCGCCTCGGCCGAGCGCTATTACCGCACGATGTACCGCAGCGCCGCGCAAAGCTGGAACTTGCGC
GACACGCACATGTTCGAGACGCTCGAAAACCTGCTCGCGGCGAAGGGCGACGGCGGCCGCGCGGTGGTGTGGGCGCACAA
CTCGCATATCGGCAACGCGGCCGCGACCGAAATGGGCGACATCCGCGACGAGCTCAACGTCGGGCAGTTGTGCCGCGAGC
GCTTCGGCGACGACGTCGCGCTGATCGGCTTCGGTACGCATGCGGGCACGGTCGCTGCCGCGACCGACTGGGGCGGCCCG
CTCGAAGTCATGCGCGTTCTTCCGTCGCGCGACGACAGCTACGAGCATGCGTTTCACGAGTCGGGGCTGCCGCGCTGTTT
CGCCGATCTGTCAGCCGACGGCGATCGCGCGCTCGTCGAATCGCTACGCGAGCCGCGGCTCGAGCGCTTCATCGGCGTGA
TCTACCGCCCCGATTCGGAGCGGCACAGTCACTACGCCGAAGCGCGGCTGTCCGCGCAGTTCGACGGCTACGTATGGTTC
GACAACACGCACGCGATCACGCCGCTGTCGACGCGCGCGACCGAGCGTTCGGTCGACCTGTATCCGTTCGGCGTGTGA

Upstream 100 bases:

>100_bases
CATGCGCCGTTCCTTCCCGCGGCACGCACGATGCTGCGCGACTCGACACTCAGGCCAACGATCGAACACCATGAATCCAT
TCGACGAAGGACGCGAGCAG

Downstream 100 bases:

>100_bases
CGGCGTCGGTAAAACGCGATTGTAGAAAAGCACCGATTCGGCGCGAAGTACGCCGCGGCGCATGCAATCCGTCTTCGCCG
CCGTCGCCCACGGTTTACCC

Product: protein-L-isoaspartate O-methyltransferase

Products: S-adenosyl-L-homocysteine; protein L-isoaspartate alpha-methylester [C]

Alternate protein names: NA

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MVERQIAARGVRDPHVLDAMRTVARHRFVPAELAEFAYSDTPLPLADDQTISQPIVVARMLEAATLSPTDRLLDVGTGSG
YAAAVASRIVAHVDSIERHASLAASAQRTLAENGFGNVDVHHADGTLGWPAHAPFDAIVAAAGGPDVPRAWREQLAIGGR
IVMPIGDERDAQRLVRITRTGATQFEHEDLGAVRFVPLIGEQGWPEHDPARDGDTARATRDIEHDAHAPYVAAPPTPLAR
TIARAARPLPEPEDPTFADAFDHLADKRIVLLGECSHGTSEFYRARAAITRRLVERHGFNIVAVEADWPDAAIVHRYVML
DERRDADPPFQRFPTWMWRNEEFAAFVEWLRAHNGRTRREARCGFHGLDMYSLSASVEAVLDYLDRSDPNAARIARERYG
CLTPWQKDPLVYGRAAMSSGFENCEDAVIAQLQDMLRGRIARAGDDDLHFDATQNARLVASAERYYRTMYRSAAQSWNLR
DTHMFETLENLLAAKGDGGRAVVWAHNSHIGNAAATEMGDIRDELNVGQLCRERFGDDVALIGFGTHAGTVAAATDWGGP
LEVMRVLPSRDDSYEHAFHESGLPRCFADLSADGDRALVESLREPRLERFIGVIYRPDSERHSHYAEARLSAQFDGYVWF
DNTHAITPLSTRATERSVDLYPFGV

Sequences:

>Translated_665_residues
MVERQIAARGVRDPHVLDAMRTVARHRFVPAELAEFAYSDTPLPLADDQTISQPIVVARMLEAATLSPTDRLLDVGTGSG
YAAAVASRIVAHVDSIERHASLAASAQRTLAENGFGNVDVHHADGTLGWPAHAPFDAIVAAAGGPDVPRAWREQLAIGGR
IVMPIGDERDAQRLVRITRTGATQFEHEDLGAVRFVPLIGEQGWPEHDPARDGDTARATRDIEHDAHAPYVAAPPTPLAR
TIARAARPLPEPEDPTFADAFDHLADKRIVLLGECSHGTSEFYRARAAITRRLVERHGFNIVAVEADWPDAAIVHRYVML
DERRDADPPFQRFPTWMWRNEEFAAFVEWLRAHNGRTRREARCGFHGLDMYSLSASVEAVLDYLDRSDPNAARIARERYG
CLTPWQKDPLVYGRAAMSSGFENCEDAVIAQLQDMLRGRIARAGDDDLHFDATQNARLVASAERYYRTMYRSAAQSWNLR
DTHMFETLENLLAAKGDGGRAVVWAHNSHIGNAAATEMGDIRDELNVGQLCRERFGDDVALIGFGTHAGTVAAATDWGGP
LEVMRVLPSRDDSYEHAFHESGLPRCFADLSADGDRALVESLREPRLERFIGVIYRPDSERHSHYAEARLSAQFDGYVWF
DNTHAITPLSTRATERSVDLYPFGV
>Mature_665_residues
MVERQIAARGVRDPHVLDAMRTVARHRFVPAELAEFAYSDTPLPLADDQTISQPIVVARMLEAATLSPTDRLLDVGTGSG
YAAAVASRIVAHVDSIERHASLAASAQRTLAENGFGNVDVHHADGTLGWPAHAPFDAIVAAAGGPDVPRAWREQLAIGGR
IVMPIGDERDAQRLVRITRTGATQFEHEDLGAVRFVPLIGEQGWPEHDPARDGDTARATRDIEHDAHAPYVAAPPTPLAR
TIARAARPLPEPEDPTFADAFDHLADKRIVLLGECSHGTSEFYRARAAITRRLVERHGFNIVAVEADWPDAAIVHRYVML
DERRDADPPFQRFPTWMWRNEEFAAFVEWLRAHNGRTRREARCGFHGLDMYSLSASVEAVLDYLDRSDPNAARIARERYG
CLTPWQKDPLVYGRAAMSSGFENCEDAVIAQLQDMLRGRIARAGDDDLHFDATQNARLVASAERYYRTMYRSAAQSWNLR
DTHMFETLENLLAAKGDGGRAVVWAHNSHIGNAAATEMGDIRDELNVGQLCRERFGDDVALIGFGTHAGTVAAATDWGGP
LEVMRVLPSRDDSYEHAFHESGLPRCFADLSADGDRALVESLREPRLERFIGVIYRPDSERHSHYAEARLSAQFDGYVWF
DNTHAITPLSTRATERSVDLYPFGV

Specific function: Catalyzes The Methyl Esterification Of L-Isoaspartyl Residues In Peptides And Proteins That Result From Spontaneous Decomposition Of Normal L-Aspartyl And L-Asparaginyl Residues. It Plays A Role In The Repair And/Or Degradation Of Damaged Proteins. This

COG id: COG2312

COG function: function code R; Erythromycin esterase homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=180, Percent_Identity=31.6666666666667, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789100, Length=195, Percent_Identity=43.5897435897436, Blast_Score=158, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71983477, Length=198, Percent_Identity=29.7979797979798, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17981723, Length=211, Percent_Identity=31.2796208530806, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007815
- InterPro:   IPR000836 [H]

Pfam domain/function: PF05139 Erythro_esteras; PF00156 Pribosyltran [H]

EC number: 2.1.1.77 [C]

Molecular weight: Translated: 73769; Mature: 73769

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVERQIAARGVRDPHVLDAMRTVARHRFVPAELAEFAYSDTPLPLADDQTISQPIVVARM
CCCHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
LEAATLSPTDRLLDVGTGSGYAAAVASRIVAHVDSIERHASLAASAQRTLAENGFGNVDV
HHHHCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HHADGTLGWPAHAPFDAIVAAAGGPDVPRAWREQLAIGGRIVMPIGDERDAQRLVRITRT
EECCCCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHC
GATQFEHEDLGAVRFVPLIGEQGWPEHDPARDGDTARATRDIEHDAHAPYVAAPPTPLAR
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEECCCCHHHH
TIARAARPLPEPEDPTFADAFDHLADKRIVLLGECSHGTSEFYRARAAITRRLVERHGFN
HHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCE
IVAVEADWPDAAIVHRYVMLDERRDADPPFQRFPTWMWRNEEFAAFVEWLRAHNGRTRRE
EEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHCCCHHCCCCHHHHHHHHHHHCCCCCHHH
ARCGFHGLDMYSLSASVEAVLDYLDRSDPNAARIARERYGCLTPWQKDPLVYGRAAMSSG
HHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCEECHHHHHCC
FENCEDAVIAQLQDMLRGRIARAGDDDLHFDATQNARLVASAERYYRTMYRSAAQSWNLR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEHHHHHHHHHHHHHHHHHCCCCC
DTHMFETLENLLAAKGDGGRAVVWAHNSHIGNAAATEMGDIRDELNVGQLCRERFGDDVA
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCEE
LIGFGTHAGTVAAATDWGGPLEVMRVLPSRDDSYEHAFHESGLPRCFADLSADGDRALVE
EEEECCCCCCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHCCCCCCHHHHH
SLREPRLERFIGVIYRPDSERHSHYAEARLSAQFDGYVWFDNTHAITPLSTRATERSVDL
HHHCHHHHHHHHEEECCCCHHHHHHHHHHHHEEECCEEEECCCCEECCCCCCCCCCCCCE
YPFGV
EECCC
>Mature Secondary Structure
MVERQIAARGVRDPHVLDAMRTVARHRFVPAELAEFAYSDTPLPLADDQTISQPIVVARM
CCCHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
LEAATLSPTDRLLDVGTGSGYAAAVASRIVAHVDSIERHASLAASAQRTLAENGFGNVDV
HHHHCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HHADGTLGWPAHAPFDAIVAAAGGPDVPRAWREQLAIGGRIVMPIGDERDAQRLVRITRT
EECCCCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHC
GATQFEHEDLGAVRFVPLIGEQGWPEHDPARDGDTARATRDIEHDAHAPYVAAPPTPLAR
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEECCCCHHHH
TIARAARPLPEPEDPTFADAFDHLADKRIVLLGECSHGTSEFYRARAAITRRLVERHGFN
HHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCE
IVAVEADWPDAAIVHRYVMLDERRDADPPFQRFPTWMWRNEEFAAFVEWLRAHNGRTRRE
EEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHCCCHHCCCCHHHHHHHHHHHCCCCCHHH
ARCGFHGLDMYSLSASVEAVLDYLDRSDPNAARIARERYGCLTPWQKDPLVYGRAAMSSG
HHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCEECHHHHHCC
FENCEDAVIAQLQDMLRGRIARAGDDDLHFDATQNARLVASAERYYRTMYRSAAQSWNLR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEHHHHHHHHHHHHHHHHHCCCCC
DTHMFETLENLLAAKGDGGRAVVWAHNSHIGNAAATEMGDIRDELNVGQLCRERFGDDVA
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCEE
LIGFGTHAGTVAAATDWGGPLEVMRVLPSRDDSYEHAFHESGLPRCFADLSADGDRALVE
EEEECCCCCCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHCCCCCCHHHHH
SLREPRLERFIGVIYRPDSERHSHYAEARLSAQFDGYVWFDNTHAITPLSTRATERSVDL
HHHCHHHHHHHHEEECCCCHHHHHHHHHHHHEEECCEEEECCCCEECCCCCCCCCCCCCE
YPFGV
EECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; protein L-isoaspartate [C]

Specific reaction: S-Adenosyl-L-methionine + protein L-isoaspartate =S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methylester [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]