Click here to switch to the map view.

The map label for this gene is btuF [H]

Identifier: 161524023

GI number: 161524023

Start: 920009

End: 920926

Strand: Reverse

Name: btuF [H]

Synonym: Bmul_0844

Alternate gene names: 161524023

Gene position: 920926-920009 (Counterclockwise)

Preceding gene: 161524024

Following gene: 161524017

Centisome position: 26.71

GC content: 71.35

Gene sequence:

>918_bases
ATGAACAGCCGCCCGTTTCGCCGGCTGCTGCCCGCAGCCATGCTCGCGCTGCTCGCGCATGCACCGCTCGCGCACGCGGA
CGTCAGCGCACGCGACGACGCCGGCCTGACCGTGACGCTGCCCGCGCCCGCGCAACGCGTGATCAGCCTCGCGCCGCACG
TGACCGAACTCGTGTACGCAGCGGGCGGCGGCGCGAAGCTCGTCGGCACCGTCACGTACAGCGACTATCCGCCGGCCGCG
AAGTCGGTGCCGCGCGTCGGCGACAACAAGGCGCTCGACCTCGAGCGGATCGCCGCGCTGCGGCCGGATCTGATCGTCGT
CTGGCGGCACGGCAATGCCGAGCGACAGACCGATGCATTGCGTGCGCTGCACATTCCGCTCTTCTTCAGCGATCCGAAGC
ATCTGGACGACATCGCGTCGTCGCTGCGCCGGCTCGGCACGCTGCTCGGCACTCAGCCGGCCGCCGATACGGCAGCCGCG
TCGTTCGAGCGCGACATCGCGGCCCTGCGCGCACGCTACGCGGCGCGCGCGCCCGTCACGGTGTTCTTCCAGGCATGGGA
CCGGCCGCTGATGACGCTGAACGGCACGCATCTGATCAGCGACGTGATCGCGCTGTGCGGCGGCCGCAACGTGTTCGCGT
CGCTGAAGCCGCTCGTGCCGACCGTCACCGACGAAGCCGTGCTCGCCACGAATCCGGAAGCGATCGTGACGACAAGCGGC
GGCGCGACGCGCTCGGATCAGGCGCTGCCGAGCCTCGAACGCTGGCGTGCATGGCCCGCGATGACGGCCGTCGCGCGCAA
CAACCTGTTCGCGATCGACGGCGATCTGCTGACGCGGCCCGCGCCGCGCATCGCGGACGGCGCAGCCGCGCTCTGTACCG
ACCTCGAGGTTGCACGCACGCGCCGGCCGGCGCACTGA

Upstream 100 bases:

>100_bases
CGTCGAGCGTGCGCATCGGGCTGCCGGGCAGCGAAGCCGAATGGCAGCGCATCGCCGACGCGCTCGCGCAATGCGTGCCG
ACGCTGGAGCGCGTATCCGC

Downstream 100 bases:

>100_bases
ACGACAATCGCGGCCGGCGTTCTGCGCGCCGGCGCGTGATGCCGCCGCATGCCGTGCCGATACCGCGCTACGCGTCGTCC
CATTGCACGACGTCCCACGC

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNSRPFRRLLPAAMLALLAHAPLAHADVSARDDAGLTVTLPAPAQRVISLAPHVTELVYAAGGGAKLVGTVTYSDYPPAA
KSVPRVGDNKALDLERIAALRPDLIVVWRHGNAERQTDALRALHIPLFFSDPKHLDDIASSLRRLGTLLGTQPAADTAAA
SFERDIAALRARYAARAPVTVFFQAWDRPLMTLNGTHLISDVIALCGGRNVFASLKPLVPTVTDEAVLATNPEAIVTTSG
GATRSDQALPSLERWRAWPAMTAVARNNLFAIDGDLLTRPAPRIADGAAALCTDLEVARTRRPAH

Sequences:

>Translated_305_residues
MNSRPFRRLLPAAMLALLAHAPLAHADVSARDDAGLTVTLPAPAQRVISLAPHVTELVYAAGGGAKLVGTVTYSDYPPAA
KSVPRVGDNKALDLERIAALRPDLIVVWRHGNAERQTDALRALHIPLFFSDPKHLDDIASSLRRLGTLLGTQPAADTAAA
SFERDIAALRARYAARAPVTVFFQAWDRPLMTLNGTHLISDVIALCGGRNVFASLKPLVPTVTDEAVLATNPEAIVTTSG
GATRSDQALPSLERWRAWPAMTAVARNNLFAIDGDLLTRPAPRIADGAAALCTDLEVARTRRPAH
>Mature_305_residues
MNSRPFRRLLPAAMLALLAHAPLAHADVSARDDAGLTVTLPAPAQRVISLAPHVTELVYAAGGGAKLVGTVTYSDYPPAA
KSVPRVGDNKALDLERIAALRPDLIVVWRHGNAERQTDALRALHIPLFFSDPKHLDDIASSLRRLGTLLGTQPAADTAAA
SFERDIAALRARYAARAPVTVFFQAWDRPLMTLNGTHLISDVIALCGGRNVFASLKPLVPTVTDEAVLATNPEAIVTTSG
GATRSDQALPSLERWRAWPAMTAVARNNLFAIDGDLLTRPAPRIADGAAALCTDLEVARTRRPAH

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=252, Percent_Identity=37.3015873015873, Blast_Score=154, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 32565; Mature: 32565

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRPFRRLLPAAMLALLAHAPLAHADVSARDDAGLTVTLPAPAQRVISLAPHVTELVYA
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHCHHHHHHHHH
AGGGAKLVGTVTYSDYPPAAKSVPRVGDNKALDLERIAALRPDLIVVWRHGNAERQTDAL
CCCCCEEEEEEEECCCCCCHHCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHH
RALHIPLFFSDPKHLDDIASSLRRLGTLLGTQPAADTAAASFERDIAALRARYAARAPVT
HHEECEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
VFFQAWDRPLMTLNGTHLISDVIALCGGRNVFASLKPLVPTVTDEAVLATNPEAIVTTSG
EEEECCCCCEEEECCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCCEEEEECC
GATRSDQALPSLERWRAWPAMTAVARNNLFAIDGDLLTRPAPRIADGAAALCTDLEVART
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHH
RRPAH
CCCCC
>Mature Secondary Structure
MNSRPFRRLLPAAMLALLAHAPLAHADVSARDDAGLTVTLPAPAQRVISLAPHVTELVYA
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHCHHHHHHHHH
AGGGAKLVGTVTYSDYPPAAKSVPRVGDNKALDLERIAALRPDLIVVWRHGNAERQTDAL
CCCCCEEEEEEEECCCCCCHHCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHH
RALHIPLFFSDPKHLDDIASSLRRLGTLLGTQPAADTAAASFERDIAALRARYAARAPVT
HHEECEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
VFFQAWDRPLMTLNGTHLISDVIALCGGRNVFASLKPLVPTVTDEAVLATNPEAIVTTSG
EEEECCCCCEEEECCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCCEEEEECC
GATRSDQALPSLERWRAWPAMTAVARNNLFAIDGDLLTRPAPRIADGAAALCTDLEVART
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHH
RRPAH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA