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The map label for this gene is cobS [H]

Identifier: 161524021

GI number: 161524021

Start: 918596

End: 919369

Strand: Direct

Name: cobS [H]

Synonym: Bmul_0842

Alternate gene names: 161524021

Gene position: 918596-919369 (Clockwise)

Preceding gene: 161524020

Following gene: 161524022

Centisome position: 26.64

GC content: 71.58

Gene sequence:

>774_bases
ATGCGTGAGCCGGCCCGCGGCGTGCGCGCGGAAGTGCGGTATTTCTTCGTCGCGCTCGGCTATTTCACGCGCGTGCCGGT
GCCGAAGGCGATCGGCTACGCGGCCGGCGATCTCGACCAGGCCGCGCGCTATTTCCCGCTCGTCGGCGCCTGCGTCGGCG
CGTGGGGTGCGCTCGTCTATCTCGCCGCGCTGCGCGTGCTGCCCGCGTCGATCGCCGTCGGGCTGTCGATGGCCGCAACC
TTGCTCGCGACGGGCGCGTTCCACGAGGACGGTCTCGCCGACAGCTGCGACGCGTTCGGCGGCGGCTATACGCGCGACGA
CGTGCTGCGCATCATGCACGACTCGCGGATCGGCACGTTCGGCGCGGTCGCGCTGATCGTCGCGCTCGGCCTGAAGTGGC
AGGCGCTGGTCGCGATGCCGCCGCTGCGCGCGGCGTGGACGATGATCGCCGCGCATGCGGCGAGCCGCGCGGCGGCGGTG
AGCCTGCTGCTGTCGCTCGACTACGTGCGGCCCGAAGGCAAGGCGAAGCCCGTCGCGCAGCGGATGAACGCGCGCGCCGC
GTGCATGGCGGCCGCATTCGGTCTGCCGTGGCTGTTCTGGCCGGACTGGCGTGCGGGCGTCGCGGCGCTCGCCGCACTGG
CGCTCGTGCGCGCGTGTGCGGCGCGCTATTTCGTGAAGCGGATCGGCGGCTATACGGGCGACTGTCTCGGCTTCGCACAG
CAGGCAAGCGAGCTCGCGATCTATCTGGTGATGCTCGGATGGATGTCGTCCTGA

Upstream 100 bases:

>100_bases
GAAGGGACGGGCGCCGCGCTCGCGCTGCCGCTCGTGCGTGCGGCAGCCGCGTTTCTCGCCGAGATGGCGAGCTTCGAATC
CGCGGGCGTCGACAATCGTG

Downstream 100 bases:

>100_bases
TCCGTCATCCGGCCGTCGCGGTCGCGTCGGGTGTCTGCTACGGACACACCGACGTGCCGCTCGCCGGCTCCGCCGACGCG
GCCGCGCGCCTGCTGCGCGA

Product: cobalamin synthase

Products: Cobalamin 5'-Phosphate; GMP; Cobalamin [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MREPARGVRAEVRYFFVALGYFTRVPVPKAIGYAAGDLDQAARYFPLVGACVGAWGALVYLAALRVLPASIAVGLSMAAT
LLATGAFHEDGLADSCDAFGGGYTRDDVLRIMHDSRIGTFGAVALIVALGLKWQALVAMPPLRAAWTMIAAHAASRAAAV
SLLLSLDYVRPEGKAKPVAQRMNARAACMAAAFGLPWLFWPDWRAGVAALAALALVRACAARYFVKRIGGYTGDCLGFAQ
QASELAIYLVMLGWMSS

Sequences:

>Translated_257_residues
MREPARGVRAEVRYFFVALGYFTRVPVPKAIGYAAGDLDQAARYFPLVGACVGAWGALVYLAALRVLPASIAVGLSMAAT
LLATGAFHEDGLADSCDAFGGGYTRDDVLRIMHDSRIGTFGAVALIVALGLKWQALVAMPPLRAAWTMIAAHAASRAAAV
SLLLSLDYVRPEGKAKPVAQRMNARAACMAAAFGLPWLFWPDWRAGVAALAALALVRACAARYFVKRIGGYTGDCLGFAQ
QASELAIYLVMLGWMSS
>Mature_257_residues
MREPARGVRAEVRYFFVALGYFTRVPVPKAIGYAAGDLDQAARYFPLVGACVGAWGALVYLAALRVLPASIAVGLSMAAT
LLATGAFHEDGLADSCDAFGGGYTRDDVLRIMHDSRIGTFGAVALIVALGLKWQALVAMPPLRAAWTMIAAHAASRAAAV
SLLLSLDYVRPEGKAKPVAQRMNARAACMAAAFGLPWLFWPDWRAGVAALAALALVRACAARYFVKRIGGYTGDCLGFAQ
QASELAIYLVMLGWMSS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=250, Percent_Identity=33.2, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 27262; Mature: 27262

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-Cobinamide; Alpha-Ribazole 5'-Phosphate; Alpha-Ribazole [C]

Specific reaction: GDP-Cobinamide + Alpha-Ribazole 5'-Phosphate (or Alpha-Ribazole) = Cobalamin 5'-Phosphate (or GDP-Cobalamin) + GMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA